MASY_ECOLI
ID MASY_ECOLI Reviewed; 533 AA.
AC P08997; Q2M6U0;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1988, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Malate synthase A;
DE Short=MSA;
DE EC=2.3.3.9;
GN Name=aceB; Synonyms=mas; OrderedLocusNames=b4014, JW3974;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3050899; DOI=10.1093/nar/16.19.9342;
RA Byrne C.R., Stokes H.W., Ward K.A.;
RT "Nucleotide sequence of the aceB gene encoding malate synthase A in
RT Escherichia coli.";
RL Nucleic Acids Res. 16:9342-9342(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3060852; DOI=10.1093/nar/16.22.10924;
RA Byrne C.R., Stokes H.W., Ward K.A.;
RT "Nucleotide sequence of the aceB gene encoding malate synthase A in
RT Escherichia coli.";
RL Nucleic Acids Res. 16:10924-10924(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC isocitrate: step 2/2.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
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DR EMBL; X12431; CAA30973.1; -; Genomic_DNA.
DR EMBL; U00006; AAC43108.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76984.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78016.1; -; Genomic_DNA.
DR PIR; A32649; SYECMA.
DR RefSeq; NP_418438.1; NC_000913.3.
DR RefSeq; WP_000138905.1; NZ_SSZK01000049.1.
DR PDB; 3CUZ; X-ray; 1.04 A; A=2-533.
DR PDB; 3CV1; X-ray; 1.68 A; A=2-533.
DR PDB; 3CV2; X-ray; 1.40 A; A/B=2-533.
DR PDBsum; 3CUZ; -.
DR PDBsum; 3CV1; -.
DR PDBsum; 3CV2; -.
DR AlphaFoldDB; P08997; -.
DR SMR; P08997; -.
DR BioGRID; 4259288; 6.
DR IntAct; P08997; 6.
DR STRING; 511145.b4014; -.
DR jPOST; P08997; -.
DR PaxDb; P08997; -.
DR PRIDE; P08997; -.
DR EnsemblBacteria; AAC76984; AAC76984; b4014.
DR EnsemblBacteria; BAE78016; BAE78016; BAE78016.
DR GeneID; 948512; -.
DR KEGG; ecj:JW3974; -.
DR KEGG; eco:b4014; -.
DR PATRIC; fig|1411691.4.peg.2699; -.
DR EchoBASE; EB0022; -.
DR eggNOG; COG2225; Bacteria.
DR HOGENOM; CLU_018928_3_0_6; -.
DR InParanoid; P08997; -.
DR OMA; CHKRGAP; -.
DR PhylomeDB; P08997; -.
DR BioCyc; EcoCyc:MALATE-SYNTHASE; -.
DR BioCyc; MetaCyc:MALATE-SYNTHASE; -.
DR UniPathway; UPA00703; UER00720.
DR EvolutionaryTrace; P08997; -.
DR PRO; PR:P08997; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IDA:EcoliWiki.
DR GO; GO:0004474; F:malate synthase activity; IDA:EcoCyc.
DR GO; GO:0006097; P:glyoxylate cycle; IBA:GO_Central.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 1.20.1220.12; -; 1.
DR Gene3D; 3.20.20.360; -; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR001465; Malate_synthase.
DR InterPro; IPR019830; Malate_synthase_CS.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR PANTHER; PTHR42902; PTHR42902; 1.
DR Pfam; PF01274; Malate_synthase; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; SSF51645; 1.
DR TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Glyoxylate bypass; Reference proteome;
KW Transferase; Tricarboxylic acid cycle.
FT CHAIN 1..533
FT /note="Malate synthase A"
FT /id="PRO_0000166875"
FT ACT_SITE 166
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 447
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT STRAND 12..15
FT /evidence="ECO:0007829|PDB:3CV2"
FT HELIX 19..24
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 27..59
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 71..74
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 91..97
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 100..107
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 109..119
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 126..140
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 145..147
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 153..155
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 162..166
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 173..179
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 182..185
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 186..204
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 210..213
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 219..235
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 243..248
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 252..255
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 258..264
FT /evidence="ECO:0007829|PDB:3CUZ"
FT TURN 265..268
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 269..273
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 277..286
FT /evidence="ECO:0007829|PDB:3CUZ"
FT TURN 287..289
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 291..293
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 298..300
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 306..321
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 325..331
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 339..341
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 343..359
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 362..368
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 369..371
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 372..383
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 402..406
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 415..433
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 438..441
FT /evidence="ECO:0007829|PDB:3CUZ"
FT STRAND 444..446
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 448..463
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 476..494
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 496..501
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 504..516
FT /evidence="ECO:0007829|PDB:3CUZ"
FT HELIX 525..529
FT /evidence="ECO:0007829|PDB:3CUZ"
SQ SEQUENCE 533 AA; 60274 MW; EB88388D7E4C5098 CRC64;
MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA RIQQQQDIDN
GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER KMVINALNAN VKVFMADFED
SLAPDWNKVI DGQINLRDAV NGTISYTNEA GKIYQLKPNP AVLICRVRGL HLPEKHVTWR
GEAIPGSLFD FALYFFHNYQ ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR
GTIKATLLIE TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK ADKSLEANNG
HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI TADQLLAPCD GERTEEGMRA
NIRVAVQYIE AWISGNGCVP IYGLMEDAAT AEISRTSIWQ WIHHQKTLSN GKPVTKALFR
QMLGEEMKVI ASELGEERFS QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA
