ID   MASY_MYXXD              Reviewed;         541 AA.
AC   P95329; Q1CYF8;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Malate synthase;
DE            EC=2.3.3.9;
GN   Name=mls; OrderedLocusNames=MXAN_6441;
OS   Myxococcus xanthus (strain DK1622).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=246197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Salmi D., Creighton C., Youderian P.;
RT   "Myxococcus xanthus fumarate hydratase, major proteosome subunit, and acyl-
RT   CoA oxidase genes.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DK1622;
RX   PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA   Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA   Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA   Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA   Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT   "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF013216; AAB97829.1; -; Genomic_DNA.
DR   EMBL; CP000113; ABF86861.1; -; Genomic_DNA.
DR   RefSeq; WP_011556373.1; NC_008095.1.
DR   AlphaFoldDB; P95329; -.
DR   SMR; P95329; -.
DR   STRING; 246197.MXAN_6441; -.
DR   EnsemblBacteria; ABF86861; ABF86861; MXAN_6441.
DR   GeneID; 41363650; -.
DR   KEGG; mxa:MXAN_6441; -.
DR   eggNOG; COG2225; Bacteria.
DR   HOGENOM; CLU_018928_3_0_7; -.
DR   OMA; CHKRGAP; -.
DR   OrthoDB; 322024at2; -.
DR   UniPathway; UPA00703; UER00720.
DR   Proteomes; UP000002402; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 1.20.1220.12; -; 1.
DR   Gene3D; 3.20.20.360; -; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   PANTHER; PTHR42902; PTHR42902; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Glyoxylate bypass; Reference proteome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..541
FT                   /note="Malate synthase"
FT                   /id="PRO_0000166876"
FT   ACT_SITE        172
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        452
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        95
FT                   /note="D -> E (in Ref. 1; AAB97829)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        385
FT                   /note="I -> L (in Ref. 1; AAB97829)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        398
FT                   /note="N -> D (in Ref. 1; AAB97829)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   541 AA;  60509 MW;  E25A8236686AA626 CRC64;
     MSDQAPSVKP PAFGPGVVVK GTWHPDYAEV LTPEALEFVA KLARNFGERR LALLERRKTV
     QAAWSKGARP HFLPETKAVR EGDWTVAPLP ADLQDRRVEI TGPVDRKMVI NALNSGANVF
     MADFEDANSP TWDNVVRGQI NLRDAVRGTI SFTAENGKHY ALNPKRAVLF VRPRGWHLPE
     RHIEIDGKPI SGSLLDFGLF FFHNAREQLA RGTGPYFYLP KMQSHLEARL WNDVFHLAQA
     ELGIPRGTIK ATVLIETLPA AFEMDEILYE LREHSAGLNC GRWDYIFSFI KTLQSDTRVV
     LPDRGQVTMD KAFLNAYSQL LIQTCHRRNV HAMGGMAAFI PIKGDAAAND AVMDKVRADK
     LREVKNGHDG TWVAHPGLVE IARDIFDSHM KGPNQLSNKR EDVKIGEAEL LKVPSGTRTE
     EGLRHNIRVG IQYTAAWLGG LGCVPLYNLM EDAATAEISR AQVWQWIHHG ASLEDGRKVT
     LTLFRDALGE EMGRLEKEGA KERYGAAHLE RARVLFEQLS TAGTFEDFLT LPAYDALEAQ
     R