MASY_NEUCR
ID MASY_NEUCR Reviewed; 542 AA.
AC P28345; Q7RV95; V5IPX4;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 25-MAY-2022, entry version 139.
DE RecName: Full=Malate synthase, glyoxysomal;
DE EC=2.3.3.9;
DE AltName: Full=Acetate utilization protein 9;
GN Name=acu-9; ORFNames=NCU10007;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1832736; DOI=10.1007/bf00260638;
RA Sandeman R.A., Hynes M.J., Fincham J.R.S., Connerton I.F.;
RT "Molecular organisation of the malate synthase genes of Aspergillus
RT nidulans and Neurospora crassa.";
RL Mol. Gen. Genet. 228:445-452(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC isocitrate: step 2/2.
CC -!- SUBCELLULAR LOCATION: Glyoxysome.
CC -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
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DR EMBL; X56672; CAA39994.1; -; Genomic_DNA.
DR EMBL; CM002236; ESA43774.1; -; Genomic_DNA.
DR EMBL; CM002236; ESA43775.1; -; Genomic_DNA.
DR PIR; S17774; S17774.
DR RefSeq; XP_011393298.1; XM_011394996.1.
DR RefSeq; XP_011393299.1; XM_011394997.1.
DR AlphaFoldDB; P28345; -.
DR SMR; P28345; -.
DR STRING; 5141.EFNCRP00000008710; -.
DR EnsemblFungi; ESA43774; ESA43774; NCU10007.
DR EnsemblFungi; ESA43775; ESA43775; NCU10007.
DR GeneID; 3875600; -.
DR KEGG; ncr:NCU10007; -.
DR VEuPathDB; FungiDB:NCU10007; -.
DR HOGENOM; CLU_018928_3_0_1; -.
DR InParanoid; P28345; -.
DR OMA; CHKRGAP; -.
DR UniPathway; UPA00703; UER00720.
DR Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR GO; GO:0005782; C:peroxisomal matrix; IBA:GO_Central.
DR GO; GO:0004474; F:malate synthase activity; IBA:GO_Central.
DR GO; GO:0006097; P:glyoxylate cycle; IBA:GO_Central.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 1.20.1220.12; -; 1.
DR Gene3D; 3.20.20.360; -; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR001465; Malate_synthase.
DR InterPro; IPR019830; Malate_synthase_CS.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR PANTHER; PTHR42902; PTHR42902; 1.
DR Pfam; PF01274; Malate_synthase; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; SSF51645; 1.
DR TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass; Glyoxysome; Peroxisome; Reference proteome; Transferase;
KW Tricarboxylic acid cycle.
FT CHAIN 1..542
FT /note="Malate synthase, glyoxysomal"
FT /id="PRO_0000166862"
FT MOTIF 540..542
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
FT ACT_SITE 168
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 449
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT CONFLICT 52..53
FT /note="QL -> HV (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 83..87
FT /note="PPAPG -> APAAPP (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 95
FT /note="I -> M (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 215
FT /note="L -> P (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 242..243
FT /note="RG -> LS (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 255
FT /note="L -> T (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 265
FT /note="Y -> F (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 269..271
FT /note="EHS -> NHT (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 276..278
FT /note="CGR -> RGG (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 284..285
FT /note="ST -> PF (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 288..294
FT /note="KFRNHSS -> EVRRFPN (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 302
FT /note="C -> D (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 311
FT /note="D -> E (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 319..325
FT /note="QTCHKRG -> KTLHRLV (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 331
FT /note="Missing (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 415
FT /note="I -> S (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 418
FT /note="E -> D (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 426
FT /note="I -> T (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 431..433
FT /note="MEA -> TEP (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 444..447
FT /note="NYLM -> KHPQ (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 467
FT /note="G -> R (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 475
FT /note="R -> H (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 479..481
FT /note="SYA -> RYP (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 488..494
FT /note="QTDELAS -> ADRQRLA (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 511
FT /note="T -> S (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
FT CONFLICT 524
FT /note="S -> C (in Ref. 1; CAA39994)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 542 AA; 61180 MW; EE0F085DD30DAC20 CRC64;
MASVETLLQG VTISGPIEEH QRKILTPQAL SFVALLHRSF NQTRKNLLER RQLRQAEIDR
GVLPDFLPET KHIRENPTWK GAPPAPGLVD RRVEITGPTD RKMVVNALNS DVYTYMADFE
DSSAPTWANM VNGQVNLYDA IRRQIDFKQG PKEYKLRTDR TLPTLIVRPR GWHLEEKHVT
IDGEPVSGSL FDFGLYFFHN AKELVQRGFG PYFYLPKMES HLEARLWNDA FNLAQDYVGI
PRGTIRGTVL IETILAAFEM DEIIYELREH SSGLNCGRWD YIFSTIKKFR NHSSFVLPDR
SCVTMTVPFM DAYVKLLIQT CHKRGVHAMG GMAAQIPIKD DKAANDKAME GVRADKLREA
RAGHDGTWVA HPALASIALE VFNKHMPTPN QLFNRREDVK IGQQDLLNMN VPGSITEEGI
RKNLNIGLGY MEAWIRGVGC VPINYLMEDA ATAEVSRSQL WQWVKHGVTT AEGKRVDKSY
ALKLLKEQTD ELASKAPQGN KFNLAAQYFA TQVTGEDYAD FLTSLLYNEI TSAGNSLPAS
KL
