ID   MASY_ORYSJ              Reviewed;         567 AA.
AC   Q7XUG1; A0A0P0WBN8; C7J1P7;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Malate synthase {ECO:0000255|RuleBase:RU000555};
DE            EC=2.3.3.9 {ECO:0000255|RuleBase:RU000555};
GN   Name=MS {ECO:0000303|PubMed:15944756};
GN   OrderedLocusNames=Os04g0486950 {ECO:0000312|EMBL:BAH92721.1},
GN   LOC_Os04g40990 {ECO:0000305};
GN   ORFNames=OJ991113_30.4 {ECO:0000312|EMBL:CAD41322.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RG   The rice full-length cDNA consortium;
RT   "Oryza sativa full length cDNA.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   INDUCTION BY SUBMERGENCE.
RX   PubMed=15944756; DOI=10.1111/j.1745-7270.2005.00060.x;
RA   Lu Y., Wu Y.R., Han B.;
RT   "Anaerobic induction of isocitrate lyase and malate synthase in submerged
RT   rice seedlings indicates the important metabolic role of the glyoxylate
RT   cycle.";
RL   Acta Biochim. Biophys. Sin. 37:406-414(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC         Evidence={ECO:0000255|RuleBase:RU000555};
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2. {ECO:0000255|RuleBase:RU000555}.
CC   -!- SUBCELLULAR LOCATION: Glyoxysome {ECO:0000305}.
CC   -!- INDUCTION: By submergence in seedlings. {ECO:0000269|PubMed:15944756}.
CC   -!- SIMILARITY: Belongs to the malate synthase family.
CC       {ECO:0000255|RuleBase:RU000555}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAH92721.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL662946; CAD41322.1; -; Genomic_DNA.
DR   EMBL; AP008210; BAH92721.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014960; BAS89802.1; -; Genomic_DNA.
DR   EMBL; AK241214; BAH00981.1; -; mRNA.
DR   RefSeq; XP_015635902.1; XM_015780416.1.
DR   AlphaFoldDB; Q7XUG1; -.
DR   SMR; Q7XUG1; -.
DR   STRING; 4530.OS04T0486950-01; -.
DR   PaxDb; Q7XUG1; -.
DR   PRIDE; Q7XUG1; -.
DR   EnsemblPlants; Os04t0486950-01; Os04t0486950-01; Os04g0486950.
DR   GeneID; 9268154; -.
DR   Gramene; Os04t0486950-01; Os04t0486950-01; Os04g0486950.
DR   KEGG; osa:9268154; -.
DR   eggNOG; KOG1261; Eukaryota.
DR   HOGENOM; CLU_018928_3_0_1; -.
DR   InParanoid; Q7XUG1; -.
DR   OMA; CHKRGAP; -.
DR   OrthoDB; 358540at2759; -.
DR   UniPathway; UPA00703; UER00720.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q7XUG1; OS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IBA:GO_Central.
DR   GO; GO:0006097; P:glyoxylate cycle; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 1.20.1220.12; -; 1.
DR   Gene3D; 3.20.20.360; -; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   PANTHER; PTHR42902; PTHR42902; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Glyoxylate bypass; Glyoxysome; Peroxisome; Reference proteome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..567
FT                   /note="Malate synthase"
FT                   /id="PRO_0000430877"
FT   MOTIF           565..567
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000305"
FT   ACT_SITE        177
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000305"
FT   ACT_SITE        466
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   567 AA;  62639 MW;  C7E30E9C17A3FE42 CRC64;
     MATNAAAPPC PCYDTPEGVD ILGRYDPEFA AILTRDSLAF VAGLQREFRG AVRYAMERRR
     EAQRRYDAGE LPRFDPATRP VREAGGWACA PVPPAIADRT VEITGPAEPR KMVINALNSG
     AKVFMADFED ALSPTWENLM RGQVNLRDAV AGTITYRDAA RGREYRLGDR PATLFVRPRG
     WHLPEAHVLV DGEPAIGCLV DFGLYFFHSH AAFRSGQGAD FGPFFYLPKM EHSREARIWK
     GVFERAEKEA GIGRGSIRAT VLVETLPAVF QMEEILHELR DHSAGLNCGR WDYIFSYVKT
     FRARPDRLLP DRALVGMAQH FMRSYSHLLI QTCHRRGVHA MGGMAAQIPI KDDAAANEAA
     LELVRKDKLR EVRAGHDGTW AAHPGLIPAI REVFEGHLGG RPNQIDAAAG DAARAGVAVT
     EEDLLQPPRG ARTVEGLRHN TRVGVQYVAA WLSGSGSVPL YNLMEDAATA EISRVQNWQW
     LRHGAVLDAG GVEVRATPEL LARVVEVEMA RVEAEVGAER FRRGRYAEAG RIFSRQCTAP
     ELDDFLTLDA YNLIVVHHPG ASSPCKL