ID   MASY_PICAN              Reviewed;         555 AA.
AC   P21360;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Malate synthase, glyoxysomal;
DE            EC=2.3.3.9;
GN   Name=MAS;
OS   Pichia angusta (Yeast) (Hansenula polymorpha).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Pichiaceae; Ogataea.
OX   NCBI_TaxID=870730;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 234-253.
RC   STRAIN=ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL
RC   Y-5445;
RX   PubMed=2349836; DOI=10.1002/yea.320060309;
RA   Bruinenberg P.G., Blaauw M., Kazemier B., Ab G.;
RT   "Cloning and sequencing of the malate synthase gene from Hansenula
RT   polymorpha.";
RL   Yeast 6:245-254(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBCELLULAR LOCATION: Glyoxysome.
CC   -!- INDUCTION: By ethanol.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
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DR   PIR; S09294; SYHQMA.
DR   AlphaFoldDB; P21360; -.
DR   SMR; P21360; -.
DR   PhylomeDB; P21360; -.
DR   UniPathway; UPA00703; UER00720.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 1.20.1220.12; -; 1.
DR   Gene3D; 3.20.20.360; -; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   PANTHER; PTHR42902; PTHR42902; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glyoxylate bypass; Glyoxysome; Peroxisome;
KW   Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..555
FT                   /note="Malate synthase, glyoxysomal"
FT                   /id="PRO_0000166863"
FT   ACT_SITE        179
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        460
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   555 AA;  63255 MW;  88137B77B2ECE303 CRC64;
     MAQINPTTLD NVKILGEVSD KPLLSKATPR DILTKDALKF IVLLHRSFNE TRKQLLENRQ
     KVQERLDAGE SLHFLEETKY IREDPNWKCL PTHPKLQCRK IEITGPPDAK MIVNAFNTDV
     FTYMTDFEDS CSPTWNNMIY GQVNLYDAIR DRIDFTNEAT GKRYKINREG RRVPVMIVRP
     RGWHMVDKHI LVDGEPISAS IMDFGLFFFH NAKFLLSQGL APFFYLPKME HYKEAQLWND
     IFCVAQDCLE IPRGTIKATV LIETLPISYQ LDEVLYALRD HSAGLNCGRW DYMFSTIKRL
     RNQKQHILPD RHQVTMTVPF MTNYVKQLIK ICHKRGVHAM GGMAATIPIK DDPEKNAAAM
     EAVRQDKLRE VLAGHDGTWI AHPGLLPTAL SVFQEHMPTP NQIHVQKNVE ITEADLVDTN
     IPDGKITMKG VSANIYIGLN YMESWLRGLG CVPINNLMED AATAEVSRLQ LYSWCKHAVK
     MDDTGKTITP EFISKLIDEE AERCAANKPN NKFKIAADCL KKEINGHTEV AEFLTTLLYD
     DIVTIGPEVD ISSLK