ID   MASY_RAPSA              Reviewed;         566 AA.
AC   Q43827;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Malate synthase, glyoxysomal;
DE            EC=2.3.3.9;
GN   Name=MLS;
OS   Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus.
OX   NCBI_TaxID=3726;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Saxa Knacker; TISSUE=Seedling;
RA   Vollack K.U., Bach T.J.;
RT   "Overexpression of radish malate synthase in yeast.";
RL   Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBCELLULAR LOCATION: Glyoxysome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
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DR   EMBL; X78852; CAA55407.1; -; mRNA.
DR   PIR; S44186; S44186.
DR   AlphaFoldDB; Q43827; -.
DR   SMR; Q43827; -.
DR   UniPathway; UPA00703; UER00720.
DR   Proteomes; UP000504610; Unplaced.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 1.20.1220.12; -; 1.
DR   Gene3D; 3.20.20.360; -; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   PANTHER; PTHR42902; PTHR42902; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Glyoxylate bypass; Glyoxysome; Peroxisome; Reference proteome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..566
FT                   /note="Malate synthase, glyoxysomal"
FT                   /id="PRO_0000166871"
FT   MOTIF           564..566
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        179
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        465
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   566 AA;  64138 MW;  807104112623FF77 CRC64;
     MELETSVYRP NAAVYDSPDG VEVRGRYDQV FAKILTREAL GFVAELQRDV SLGMLRYAWS
     AVERQNVVTT FVLSLGLTLP LSSSEIGEWV CSVCSPPAVA DRRVEITGPV ERKMIINALN
     SGAKVFMADF EDALSPSWEN LMKGQVNLKD AVDGTITFHD KARNKVYKLN DQVAKLFVRP
     RGWHLPEAHI LIDGEPAIGC LVDFGLYFFH NYSKFRQTQG SGYGPFFYLP KMEHSREAKI
     WNSVFERAEK MAGIERGSIR ATVLIETLPA VFQMNEILYE LRDHSVGLNC GRWDYIFSYV
     KTFQAHPDRL LPDRVLVGMG QHFMRSYSDL LIRTCHKRGV HAWEGMAAQI AIRDDPKAND
     MALDLVKKDK LRQVRAGHDG TWAAHPGLIP ICMDAFGHMG KNPNQIKSMK RDDASAITEE
     DLLQIPRGVR TLDGLRLNTR VGIQYLAAWL TGSGSVPLYN LTDEDAATAE ISRVQNWQWI
     RYGVELKRRT GLEVRVSKEL FGRVVEEEME RIEKEVGKER SLREECIRKL ARCLQSSVTA
     AELDDFLTLA VYDHIVAHYP INVSRL