ID   MASY_RICCO              Reviewed;         567 AA.
AC   P17815;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Malate synthase, glyoxysomal;
DE            EC=2.3.3.9;
OS   Ricinus communis (Castor bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC   Ricinus.
OX   NCBI_TaxID=3988;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Endosperm;
RX   PubMed=2103467; DOI=10.1007/bf00019167;
RA   Rodriguez D., Ginger R.S., Baker A., Northcote D.H.;
RT   "Nucleotide sequence analysis of a cDNA clone encoding malate synthase of
RT   castor bean (Ricinus communis) reveals homology to DAL7, a gene involved in
RT   allantoin degradation in Saccharomyces cerevisiae.";
RL   Plant Mol. Biol. 15:501-504(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBCELLULAR LOCATION: Glyoxysome.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X52806; CAA36994.1; -; mRNA.
DR   PIR; S14677; SYCSM2.
DR   RefSeq; NP_001310646.1; NM_001323717.1.
DR   AlphaFoldDB; P17815; -.
DR   SMR; P17815; -.
DR   STRING; 3988.XP_002511225.1; -.
DR   GeneID; 8266340; -.
DR   KEGG; rcu:8266340; -.
DR   eggNOG; KOG1261; Eukaryota.
DR   BRENDA; 2.3.3.9; 1204.
DR   UniPathway; UPA00703; UER00720.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 1.20.1220.12; -; 1.
DR   Gene3D; 3.20.20.360; -; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   PANTHER; PTHR42902; PTHR42902; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Glyoxylate bypass; Glyoxysome; Peroxisome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..567
FT                   /note="Malate synthase, glyoxysomal"
FT                   /id="PRO_0000166872"
FT   MOTIF           565..567
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        182
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        468
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   567 AA;  64263 MW;  3888DCAF87CD36E2 CRC64;
     MRYDTYGDSA PIKKTGAGYD VPEGVDIRGR YDGEFAKILT RDALQFVADL QREFRNRIRY
     AIECRKEAKS RYNAGALPGF EHPATKYIRE GEWTCAPVPP AVADRKVEIT GPVERKMIIN
     ALNSGAKVFM ADFEDALSPS WENLMRGQVN LRDAVNGTIS FHDKARNRVY KLNDQIAKLF
     VRPRGWHLPE AHILIDGEPA TGCLVDFGLY FYHNYAAFRR NQGAGYGPFF YLPKMEHSRE
     AKIWNCVFEK AEKMAGIERG SIRATVLIET LPAVFQMNEI LYELRDHSVG LNCGRWDYIF
     SYVKTFQAHP DRPLPDRVQV GMTQHFMKSY SDLLVWTCHR RGVHAMGGMA AQIPIRDDPA
     ANKAALELVR KDKLREVKAG HDGTWAAHPG LIPACMEVFA NNMGNTPHQI QAMKREDAAN
     ITEEDLIQRP RGVRTLEGLR LNTRVGIQYL AAWLTGTGSV PLYNLMEDAA TAEISRVQNW
     QWLKYGVELD GDGLGVKVTF DLLGRVVEDE MARIEREVGK EKFKKGMYKE ACKMFVRQCA
     APTLDDFLTL DAYNNIVIHY PKGSSRL