ID   MASY_STRAE              Reviewed;         543 AA.
AC   P77947;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Malate synthase;
DE            EC=2.3.3.9;
GN   Name=aceB;
OS   Streptomyces arenae.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=29301;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=Tu469;
RX   PubMed=9133598; DOI=10.1016/s0378-1119(96)00817-7;
RA   Huettner S., Mecke D., Froehlich K.-U.;
RT   "Gene cloning and sequencing, and enzyme purification of the malate
RT   synthase of Streptomyces arenae.";
RL   Gene 188:239-246(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
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DR   EMBL; U63518; AAB53938.1; -; Genomic_DNA.
DR   AlphaFoldDB; P77947; -.
DR   SMR; P77947; -.
DR   UniPathway; UPA00703; UER00720.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1220.12; -; 1.
DR   Gene3D; 3.20.20.360; -; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   PANTHER; PTHR42902; PTHR42902; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Glyoxylate bypass; Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..543
FT                   /note="Malate synthase"
FT                   /id="PRO_0000166877"
SQ   SEQUENCE   543 AA;  61408 MW;  60829A40F62A0D85 CRC64;
     MSAPAPSPLA IVDAEPLPRQ EEVLTDAALA FVAELLHRRF TRAVTNSSPR RADAAREIAR
     TSTLDFLPET AAVRADDSWR VAGPAALNDR RVEITGPTDR KMTINALNSG AKVWLADFEE
     PSAPTWENVV LGQVNLSDAY TRNIDFTDER TGKTYALRPL KSWRPSSWPR GWHLNERHLV
     DPDGSFGARR VVVDFGLYFF HNAQRLLDLG KGPYFYLPKT ESHLEARLWN DVFVFAQDYV
     GIPQGTVRAT VLIETITAAY EMEEILYELR DHASGLNAGR WDYLFSIVKN FPATRPKFVL
     PDRNVTMTKL TMTAFMRRYT NCSSYCHNAR ASAAIGGMAA FIPSRDAEVN KVAFEKVRAD
     KDREANDGFD GSWVAHPDLV PIAMESFDRC WHRPNQKDRL REDVHVEAAD LIAVDSLEAT
     TYAGSSSTAV QVGIRYIEAW LRGLGRVAIF NLMEDAATAE ISRSQIWQWI NAGVEFEHGE
     KATPIWPRKS RRGRKWRPVR EELGEEPFAA RDWQHAHDLV VQVSLDEDYA DFLTLPAYER
     LRG