ID   MASY_STRCL              Reviewed;         541 AA.
AC   Q9ZH77;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Malate synthase;
DE            EC=2.3.3.9;
GN   Name=aceB;
OS   Streptomyces clavuligerus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1901;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL
RC   3585 / VKM Ac-602;
RX   PubMed=9846758; DOI=10.1099/00221287-144-11-3229;
RA   Chan M., Sim T.S.;
RT   "Malate synthase from Streptomyces clavuligerus NRRL3585: cloning,
RT   molecular characterization and its control by acetate.";
RL   Microbiology 144:3229-3237(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
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DR   EMBL; AF070989; AAC83648.1; -; Genomic_DNA.
DR   RefSeq; WP_003954464.1; NZ_CP032052.1.
DR   AlphaFoldDB; Q9ZH77; -.
DR   SMR; Q9ZH77; -.
DR   STRING; 443255.SCLAV_5001; -.
DR   GeneID; 61468938; -.
DR   eggNOG; COG2225; Bacteria.
DR   OMA; CHKRGAP; -.
DR   OrthoDB; 322024at2; -.
DR   BRENDA; 2.3.3.9; 5988.
DR   UniPathway; UPA00703; UER00720.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 1.20.1220.12; -; 1.
DR   Gene3D; 3.20.20.360; -; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   PANTHER; PTHR42902; PTHR42902; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Glyoxylate bypass; Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..541
FT                   /note="Malate synthase"
FT                   /id="PRO_0000166878"
FT   ACT_SITE        169
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        454
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   541 AA;  60012 MW;  26206E19BB4B8395 CRC64;
     MSASAPSRPA VVTAPAVPRQ DEVLTEAALV FLAELHRRFT PRRDELLARR ARRRAEISRT
     GTLDFLPETA HVRADDSWRV APAPPALQDR RVEITGPADR RMTVNALNSG ARVWLADFED
     SSAPTWENVI SGQLALSDAY HRRIDFTDER SGKSYALRPD AELATVVMRP RGWHLPERHL
     TGPDGGVLPG ALVDFGLCFF HTARRLLDLG KGPYFYLPKL ESHQEARLWN DIFLFAQEQL
     GIPRGTIRAT VLIETITAAY EMEEILYELR EHASGLNAGR WDYLFSIIKN FRDAGPGFVL
     PDRNAITMTA PFMRAYTELL VRTCHRRGAH AIGGMAAFIP SRRDPEINRA AFEKVRADKD
     REAADGFDGS WVAHPDLVPV ALASFDAVLG DRPHQKDRLR EEVSVTAAEL IAVGSPAARP
     TAAGLLNAVR VGIRYIEAWL RGTGAVAIFH LMEDAATAEI SRSQIWQWIN AGVVLENGER
     VTPALVRTLA AGELGALRET LGGEEYAAGR WQEAHDLLLR VALDEEYADF LTLPAYERLT
     G