MAT1_BEMTA
ID MAT1_BEMTA Reviewed; 461 AA.
AC P0DUQ3; A0A861LVW7;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 1.
DT 03-AUG-2022, entry version 3.
DE RecName: Full=Phenolic glucoside malonyltransferase 1 {ECO:0000303|PubMed:33770502};
DE Short=BtPMaT1 {ECO:0000303|PubMed:33770502};
DE EC=2.3.1.- {ECO:0000269|PubMed:33770502};
DE EC=2.3.1.116 {ECO:0000269|PubMed:33770502};
GN Name=MAT1 {ECO:0000303|PubMed:33770502};
OS Bemisia tabaci (Sweetpotato whitefly) (Aleurodes tabaci).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aleyrodoidea;
OC Aleyrodidae; Aleyrodinae; Bemisia.
OX NCBI_TaxID=7038;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=MED;
RX PubMed=33770502; DOI=10.1016/j.cell.2021.02.014;
RA Xia J., Guo Z., Yang Z., Han H., Wang S., Xu H., Yang X., Yang F., Wu Q.,
RA Xie W., Zhou X., Dermauw W., Turlings T.C.J., Zhang Y.;
RT "Whitefly hijacks a plant detoxification gene that neutralizes plant
RT toxins.";
RL Cell 0:0-0(2021).
CC -!- FUNCTION: Phenolic glucoside malonyltransferase that neutralizes
CC phenolic glycosides in host plants (PubMed:33770502). Catalyzes the
CC transfer of a malonyl group from malonyl-CoA to the phenolic
CC glycosides, leading to their detoxification (PubMed:33770502). Phenolic
CC glycosides, which are among the most abundant plant secondary
CC metabolites, act as plant defense compounds: they strongly affect
CC growth, development and behavior of insect herbivores
CC (PubMed:33770502). Has malonyltransferase activity against flavonoids
CC kaempferol 3-O-glucoside, kaempferol 7-O-glucoside, isoquercetin
CC (quercetin 3-O-beta-D-glucopyranoside), apigetrin (apigenin 7-O-beta-D-
CC glucoside) and prunin (naringenin 7-O-beta-D-glucoside)
CC (PubMed:33770502). Also has activity toward non-flavonoid rhaponticin,
CC but with lower efficiency (PubMed:33770502).
CC {ECO:0000269|PubMed:33770502}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a flavonol 3-O-beta-D-glucoside + malonyl-CoA = a flavonol 3-
CC O-(6-O-malonyl-beta-D-glucoside) + CoA; Xref=Rhea:RHEA:20085,
CC ChEBI:CHEBI:16816, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384,
CC ChEBI:CHEBI:58034; EC=2.3.1.116;
CC Evidence={ECO:0000269|PubMed:33770502};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20086;
CC Evidence={ECO:0000269|PubMed:33770502};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=kaempferol 3-O-beta-D-glucoside + malonyl-CoA = CoA +
CC kaempferol 3-O-(6-O-malonyl-beta-D-glucoside); Xref=Rhea:RHEA:67336,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:169942,
CC ChEBI:CHEBI:169943; Evidence={ECO:0000269|PubMed:33770502};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67337;
CC Evidence={ECO:0000269|PubMed:33770502};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=malonyl-CoA + quercetin 3-O-beta-D-glucoside = CoA + quercetin
CC 3-O-(6-O-malonyl-beta-D-glucoside); Xref=Rhea:RHEA:67340,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:144437,
CC ChEBI:CHEBI:169948; Evidence={ECO:0000269|PubMed:33770502};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67341;
CC Evidence={ECO:0000269|PubMed:33770502};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a flavonol 7-O-beta-D-glucoside + malonyl-CoA = a flavonol 7-
CC O-(6-O-malonyl-beta-D-glucoside) + CoA; Xref=Rhea:RHEA:58796,
CC ChEBI:CHEBI:52144, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384,
CC ChEBI:CHEBI:142805; Evidence={ECO:0000269|PubMed:33770502};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:58797;
CC Evidence={ECO:0000269|PubMed:33770502};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2S)-naringenin 7-O-beta-D-glucoside + malonyl-CoA = (2S)-
CC naringenin 7-O-(6-O-malonyl-beta-D-glucoside) + CoA;
CC Xref=Rhea:RHEA:67356, ChEBI:CHEBI:28327, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:169950;
CC Evidence={ECO:0000269|PubMed:33770502};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67357;
CC Evidence={ECO:0000269|PubMed:33770502};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=kaempferol 7-O-beta-D-glucoside + malonyl-CoA = CoA +
CC kaempferol 7-O-(6-O-malonyl-beta-D-glucoside); Xref=Rhea:RHEA:67344,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:169944,
CC ChEBI:CHEBI:169945; Evidence={ECO:0000269|PubMed:33770502};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67345;
CC Evidence={ECO:0000269|PubMed:33770502};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=apigenin 7-O-beta-D-glucoside + malonyl-CoA = apigenin 7-O-(6-
CC O-malonyl-beta-D-glucoside) + CoA; Xref=Rhea:RHEA:67348,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:77722,
CC ChEBI:CHEBI:169949; Evidence={ECO:0000269|PubMed:33770502};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67349;
CC Evidence={ECO:0000269|PubMed:33770502};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=malonyl-CoA + rhaponticin = 6-O-malonyl-rhaponticin + CoA;
CC Xref=Rhea:RHEA:67352, ChEBI:CHEBI:8824, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:169946;
CC Evidence={ECO:0000269|PubMed:33770502};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67353;
CC Evidence={ECO:0000269|PubMed:33770502};
CC -!- TISSUE SPECIFICITY: Expressed in all tissues (PubMed:33770502). Most
CC highly expressed in the abdomen and especially in the gut
CC (PubMed:33770502). {ECO:0000269|PubMed:33770502}.
CC -!- DEVELOPMENTAL STAGE: Expressed in all developmental stages with higher
CC expression in adults (PubMed:33770502). Also detected in the egg stage
CC to a much lower level (PubMed:33770502). {ECO:0000269|PubMed:33770502}.
CC -!- MISCELLANEOUS: MAT1 protein-coding gene was acquired from plants
CC through a plant-to-insect horizontal gene transfer event.
CC {ECO:0000269|PubMed:33770502}.
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. Phenolic
CC glucoside malonyltransferase subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=For the sake of sap - Issue
CC 244 of February 2022;
CC URL="https://web.expasy.org/spotlight/back_issues/244/";
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DR EMBL; MN756010; QNN26309.1; -; mRNA.
DR SMR; P0DUQ3; -.
DR GO; GO:0047165; F:flavonol-3-O-beta-glucoside O-malonyltransferase activity; IDA:UniProtKB.
DR GO; GO:0050736; F:O-malonyltransferase activity; IDA:UniProtKB.
DR GO; GO:0098754; P:detoxification; IDA:UniProtKB.
DR GO; GO:0009636; P:response to toxic substance; IDA:UniProtKB.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 1: Evidence at protein level;
KW Acyltransferase; Detoxification; Transferase.
FT CHAIN 1..461
FT /note="Phenolic glucoside malonyltransferase 1"
FT /id="PRO_0000453286"
FT MOTIF 167..171
FT /note="HXXXD motif"
FT /evidence="ECO:0000250|UniProtKB:Q589Y0"
FT MOTIF 400..404
FT /note="DFGWG motif"
FT /evidence="ECO:0000250|UniProtKB:Q589Y0"
FT ACT_SITE 167
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8W1W9"
FT ACT_SITE 400
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8W1W9"
FT BINDING 281..282
FT /ligand="malonyl-CoA"
FT /ligand_id="ChEBI:CHEBI:57384"
FT /evidence="ECO:0000250|UniProtKB:Q589Y0"
SQ SEQUENCE 461 AA; 51733 MW; 6C61394F12DB38DC CRC64;
MSISSSVAVL NVVQVSPPTA PVNNAFQDRI SLTHFDLLAL RAPPNQRLFF YETHLPISAF
AETVIPKLRD SLSLTLQNFR PLAGTLIWSL HSDEPYIRIK DDDSVPLTIA ETDADPQKLF
DDPFQQETDL QQLLPPLRVS ETEASLLALQ ITLFPSGDIC LGITFHHAAQ DGASLALFLK
SWAHICRHGD DPPLPQNLIP IFDRDFIDDP KNIKQLFLDH LLTPLTPGGP RNRSVKPMEK
PFNDRMHGSF RLTVDDIENL RRRITSLQVQ NTSQEPPVRM STVVVTCAYV LTCFVKAGLT
KKHVRFILPA DLRKRLQPPV PDNYYGNCVF GCTVDMSSDD LAGQDGLVVA AKTISSVVSE
LDANDHRTFF ENFLLNNTIS QEETKVGVGG SIYFSLDEKD FGWGGPKHLK NVPPWPNHIY
LAERRDGDKG VDFCLMLAKQ EMAEFESKFL DDLKLLEKRS C
