MATP_ECOLI
ID MATP_ECOLI Reviewed; 150 AA.
AC P0A8N0; P45569; P75868; Q83RX3;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Macrodomain Ter protein {ECO:0000255|HAMAP-Rule:MF_01073};
GN Name=matP {ECO:0000255|HAMAP-Rule:MF_01073}; Synonyms=ycbG;
GN OrderedLocusNames=b0956, JW0939;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-150.
RC STRAIN=K12;
RX PubMed=6253901; DOI=10.1093/nar/8.13.3011;
RA Beck E., Bremer E.;
RT "Nucleotide sequence of the gene ompA coding the outer membrane protein II
RT of Escherichia coli K-12.";
RL Nucleic Acids Res. 8:3011-3027(1980).
RN [5]
RP IDENTIFICATION.
RX PubMed=7567469; DOI=10.1093/nar/23.17.3554;
RA Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.;
RT "Detection of new genes in a bacterial genome using Markov models for three
RT gene classes.";
RL Nucleic Acids Res. 23:3554-3562(1995).
RN [6]
RP FUNCTION, DNA-BINDING, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE,
RP AND GENE NAME.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=18984159; DOI=10.1016/j.cell.2008.08.031;
RA Mercier R., Petit M.A., Schbath S., Robin S., El Karoui M., Boccard F.,
RA Espeli O.;
RT "The MatP/matS site-specific system organizes the terminus region of the E.
RT coli chromosome into a macrodomain.";
RL Cell 135:475-485(2008).
CC -!- FUNCTION: Required for spatial organization of the terminus region of
CC the chromosome (Ter macrodomain) during the cell cycle. Prevents early
CC segregation of duplicated Ter macrodomains during cell division. Binds
CC specifically to matS, which is a 13 bp signature motif repeated within
CC the Ter macrodomain. {ECO:0000255|HAMAP-Rule:MF_01073,
CC ECO:0000269|PubMed:18984159}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01073,
CC ECO:0000269|PubMed:18984159}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01073,
CC ECO:0000269|PubMed:18984159}. Note=Accumulates in the cell as a
CC discrete focus that colocalizes with the Ter macrodomain.
CC -!- DISRUPTION PHENOTYPE: Mutants grown in rich medium show severe defects
CC in chromosome segregation and cell division.
CC {ECO:0000269|PubMed:18984159}.
CC -!- SIMILARITY: Belongs to the MatP family. {ECO:0000255|HAMAP-
CC Rule:MF_01073}.
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DR EMBL; U00096; AAC74042.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35714.1; -; Genomic_DNA.
DR EMBL; V00307; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; C64836; C64836.
DR RefSeq; NP_415476.1; NC_000913.3.
DR RefSeq; WP_000877161.1; NZ_STEB01000006.1.
DR PDB; 4D8J; X-ray; 3.55 A; A/B/C/D/G/H/K/L=1-150.
DR PDBsum; 4D8J; -.
DR AlphaFoldDB; P0A8N0; -.
DR SMR; P0A8N0; -.
DR BioGRID; 4260031; 9.
DR BioGRID; 849944; 12.
DR IntAct; P0A8N0; 24.
DR STRING; 511145.b0956; -.
DR jPOST; P0A8N0; -.
DR PaxDb; P0A8N0; -.
DR PRIDE; P0A8N0; -.
DR EnsemblBacteria; AAC74042; AAC74042; b0956.
DR EnsemblBacteria; BAA35714; BAA35714; BAA35714.
DR GeneID; 58461146; -.
DR GeneID; 945570; -.
DR KEGG; ecj:JW0939; -.
DR KEGG; eco:b0956; -.
DR PATRIC; fig|1411691.4.peg.1318; -.
DR EchoBASE; EB2699; -.
DR eggNOG; COG3120; Bacteria.
DR HOGENOM; CLU_142157_0_0_6; -.
DR OMA; QEAHWKW; -.
DR PhylomeDB; P0A8N0; -.
DR BioCyc; EcoCyc:EG12855-MON; -.
DR PRO; PR:P0A8N0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR CollecTF; EXPREG_000007b0; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0097047; C:DNA replication termination region; IDA:EcoCyc.
DR GO; GO:0005543; F:phospholipid binding; IDA:EcoCyc.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:EcoCyc.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IMP:EcoCyc.
DR GO; GO:0051276; P:chromosome organization; IDA:EcoCyc.
DR GO; GO:0007059; P:chromosome segregation; IMP:EcoCyc.
DR GO; GO:0051289; P:protein homotetramerization; IDA:EcoCyc.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 1.20.1270.380; -; 1.
DR HAMAP; MF_01073; MatP; 1.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR009390; MatP.
DR InterPro; IPR035375; MatP_C.
DR InterPro; IPR035087; MatP_N.
DR InterPro; IPR038339; MatP_N_sf.
DR Pfam; PF06303; MatP; 1.
DR Pfam; PF17414; MatP_C; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Cytoplasm; DNA-binding;
KW Reference proteome.
FT CHAIN 1..150
FT /note="Macrodomain Ter protein"
FT /id="PRO_0000070347"
SQ SEQUENCE 150 AA; 17693 MW; B2E971E41317CF31 CRC64;
MKYQQLENLE SGWKWKYLVK KHREGELITR YIEASAAQEA VDVLLSLENE PVLVNGWIDK
HMNPELVNRM KQTIRARRKR HFNAEHQHTR KKSIDLEFIV WQRLAGLAQR RGKTLSETIV
QLIEDAENKE KYANKMSSLK QDLQALLGKE
