MATRX_BDV1
ID MATRX_BDV1 Reviewed; 142 AA.
AC P0C794; P52637;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 1.
DT 03-AUG-2022, entry version 48.
DE RecName: Full=Matrix protein;
DE AltName: Full=gp18;
DE AltName: Full=p16;
GN Name=M;
OS Borna disease virus 1 (BoDV-1).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Bornaviridae; Orthobornavirus.
OX NCBI_TaxID=1714621;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
OH NCBI_TaxID=9352; Bradypodidae (three-fingered sloths).
OH NCBI_TaxID=9925; Capra hircus (Goat).
OH NCBI_TaxID=9850; Cervidae (deer).
OH NCBI_TaxID=109474; Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew).
OH NCBI_TaxID=9788; Equidae (horses).
OH NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH NCBI_TaxID=56798; Hexaprotodon liberiensis (Pygmy hippopotamus) (Choeropsis liberiensis).
OH NCBI_TaxID=9844; Lama glama (Llama).
OH NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
OH NCBI_TaxID=9940; Ovis aries (Sheep).
OH NCBI_TaxID=8801; Struthio camelus (Common ostrich).
OH NCBI_TaxID=9455; Varecia variegata (Black-and-white ruffed lemur) (Lemur variegatus).
OH NCBI_TaxID=30538; Vicugna pacos (Alpaca) (Lama pacos).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=7906311; DOI=10.1128/jvi.68.3.1382-1396.1994;
RA Cubitt B., Oldstone C., de la Torre J.C.;
RT "Sequence and genome organization of Borna disease virus.";
RL J. Virol. 68:1382-1396(1994).
RN [2]
RP SEQUENCE REVISION TO 12.
RA Cubitt B., Oldstone C., de la Torre J.C.;
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate H1, and Isolate H2;
RA del la Torre J.C., Bode L., Durrwald R., Cubitt B., Ludwig H.;
RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Various strains;
RA Zimmermann W., Kokorsch J., Lundgren A.L., Ludwig H.;
RL Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 2-10; 24-31 AND 102-134.
RC STRAIN=strain HE/80;
RX PubMed=7933072; DOI=10.1128/jvi.68.11.6918-6923.1994;
RA Kliche S., Briese T., Henschen A.H., Stitz L., Lipkin W.I.;
RT "Characterization of a Borna disease virus glycoprotein, gp18.";
RL J. Virol. 68:6918-6923(1994).
RN [6]
RP CHARACTERIZATION, AND SUBCELLULAR LOCATION.
RC STRAIN=strain He/80;
RX PubMed=11711600; DOI=10.1128/jvi.75.24.12098-12104.2001;
RA Kraus I., Eickmann M., Kiermayer S., Scheffczik H., Fluess M., Richt J.A.,
RA Garten W.;
RT "Open reading frame III of borna disease virus encodes a nonglycosylated
RT matrix protein.";
RL J. Virol. 75:12098-12104(2001).
RN [7]
RP REVIEW.
RX PubMed=11815287; DOI=10.2741/a789;
RA Ikuta K., Ibrahim M.S., Kobayashi T., Tomonaga K.;
RT "Borna disease virus and infection in humans.";
RL Front. Biosci. 7:470-495(2002).
RN [8] {ECO:0007744|PDB:3F1J}
RP X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS), RNA-BINDING, AND SUBUNIT.
RC STRAIN=He/80;
RX PubMed=19237566; DOI=10.1073/pnas.0808101106;
RA Neumann P., Lieber D., Meyer S., Dautel P., Kerth A., Kraus I., Garten W.,
RA Stubbs M.T.;
RT "Crystal structure of the Borna disease virus matrix protein (BDV-M)
RT reveals ssRNA binding properties.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:3710-3715(2009).
CC -!- FUNCTION: Plays a crucial role in virion assembly and budding.
CC {ECO:0000305}.
CC -!- SUBUNIT: Homooligomer (By similarity). Homotetramer (PubMed:19237566).
CC Interacts with phosphoprotein P (By similarity). Binds to ssRNA
CC (PubMed:19237566). {ECO:0000250|UniProtKB:P0C795,
CC ECO:0000269|PubMed:19237566}.
CC -!- INTERACTION:
CC P0C794; P0C794: M; NbExp=2; IntAct=EBI-15760176, EBI-15760176;
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host cytoplasm
CC {ECO:0000250|UniProtKB:P0C795}. Host cell membrane; Peripheral membrane
CC protein {ECO:0000269|PubMed:11711600}. Note=During viral budding,
CC associates with the inner side of the plasma membrane of infected cells
CC (PubMed:11711600). Found in nuclear punctate structures that have been
CC proposed to be the sites of viral replication and transcription, also
CC termed viral speckles of transcripts (vSPOTs) (By similarity).
CC {ECO:0000250|UniProtKB:P0C795, ECO:0000269|PubMed:11711600}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=Matrix protein;
CC IsoId=P0C794-1; Sequence=Displayed;
CC Name=Envelope glycoprotein p57 precursor;
CC IsoId=Q8BB27-1; Sequence=External;
CC Name=Large structural protein;
CC IsoId=Q8JMN0-1; Sequence=External;
CC -!- PTM: Not glycosylated. {ECO:0000269|PubMed:11711600}.
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DR EMBL; L76228; AAA91188.1; -; Genomic_RNA.
DR EMBL; L76230; AAA91189.1; -; Genomic_RNA.
DR EMBL; L27077; AAA20665.2; -; Genomic_RNA.
DR EMBL; U94865; AAB53714.1; -; Genomic_RNA.
DR EMBL; U94869; AAB53718.1; -; Genomic_RNA.
DR EMBL; U94873; AAB53722.1; -; Genomic_RNA.
DR EMBL; U94877; AAB53726.1; -; Genomic_RNA.
DR EMBL; U94881; AAB53730.1; -; Genomic_RNA.
DR RefSeq; NP_042022.1; NC_001607.1.
DR PDB; 3F1J; X-ray; 2.65 A; A=1-142.
DR PDB; 4HI5; X-ray; 3.60 A; A=1-142.
DR PDB; 4HI6; X-ray; 2.20 A; A/B/C/D=1-142.
DR PDB; 4HIT; X-ray; 2.40 A; A/B/C/D=1-142.
DR PDB; 4HIU; X-ray; 3.30 A; A=1-142.
DR PDB; 4HIW; X-ray; 2.90 A; A=1-142.
DR PDB; 4HIY; X-ray; 3.31 A; A=1-142.
DR PDBsum; 3F1J; -.
DR PDBsum; 4HI5; -.
DR PDBsum; 4HI6; -.
DR PDBsum; 4HIT; -.
DR PDBsum; 4HIU; -.
DR PDBsum; 4HIW; -.
DR PDBsum; 4HIY; -.
DR SMR; P0C794; -.
DR GeneID; 26799165; -.
DR KEGG; vg:26799165; -.
DR EvolutionaryTrace; P0C794; -.
DR Proteomes; UP000185272; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-KW.
DR Gene3D; 2.70.20.40; -; 1.
DR InterPro; IPR032414; BDV_M.
DR InterPro; IPR038520; BDV_M_sf.
DR Pfam; PF16520; BDV_M; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Direct protein sequencing;
KW Host cell membrane; Host cytoplasm; Host membrane; Membrane;
KW Viral matrix protein; Virion.
FT CHAIN 1..142
FT /note="Matrix protein"
FT /id="PRO_0000079206"
FT BINDING 64..66
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007744|PDB:4HIU"
FT BINDING 117
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007744|PDB:4HIU"
FT VARIANT 108
FT /note="E -> D (in strain: Isolate H1 and Isolate H2)"
FT CONFLICT 12
FT /note="D -> G (in Ref. 2; AAA20665)"
FT /evidence="ECO:0000305"
FT HELIX 12..14
FT /evidence="ECO:0007829|PDB:4HI6"
FT STRAND 21..28
FT /evidence="ECO:0007829|PDB:4HI6"
FT STRAND 29..33
FT /evidence="ECO:0007829|PDB:3F1J"
FT STRAND 38..47
FT /evidence="ECO:0007829|PDB:4HI6"
FT HELIX 57..60
FT /evidence="ECO:0007829|PDB:4HI6"
FT STRAND 61..67
FT /evidence="ECO:0007829|PDB:4HI6"
FT STRAND 69..71
FT /evidence="ECO:0007829|PDB:4HI6"
FT STRAND 73..79
FT /evidence="ECO:0007829|PDB:4HI6"
FT HELIX 81..90
FT /evidence="ECO:0007829|PDB:4HI6"
FT STRAND 91..95
FT /evidence="ECO:0007829|PDB:4HI6"
FT STRAND 99..104
FT /evidence="ECO:0007829|PDB:4HI6"
FT HELIX 107..115
FT /evidence="ECO:0007829|PDB:4HI6"
FT TURN 116..118
FT /evidence="ECO:0007829|PDB:4HI6"
FT STRAND 120..128
FT /evidence="ECO:0007829|PDB:4HI6"
FT HELIX 134..139
FT /evidence="ECO:0007829|PDB:4HI6"
SQ SEQUENCE 142 AA; 16258 MW; 5EE3B27113FF771D CRC64;
MNSKHSYVEL KDKVIVPGWP TLMLEIDFVG GTSRNQFLNI PFLSVKEPLQ LPREKKLTDY
FTIDVEPAGH SLVNIYFQID DFLLLTLNSL SVYKDPIRKY MFLRLNKEQS KHAINAAFNV
FSYRLRNIGV GPLGPDIRSS GP
