ID   MATRX_CHAV              Reviewed;         229 AA.
AC   Q9WH76;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   23-FEB-2022, entry version 80.
DE   RecName: Full=Matrix protein;
GN   Name=M;
OS   Chandipura virus (strain I653514) (CHPV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae;
OC   Vesiculovirus.
OX   NCBI_TaxID=11273;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=7198; Phlebotominae (sandflies).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=10595413; DOI=10.1023/a:1008188730975;
RA   Taylor A., Easton A.J., Marriott A.C.;
RT   "Matrix protein of Chandipura virus inhibits transcription from an RNA
RT   polymerase II promoter.";
RL   Virus Genes 19:223-228(1999).
CC   -!- FUNCTION: Plays a major role in assembly and budding of virion.
CC       Condensates the ribonucleocapsid core during virus assembly. Shut off
CC       cellular transcription by inhibiting mRNA nuclear export through direct
CC       interaction with host RAE1-NUP98 complex. This shutoff presumably
CC       inhibit interferon signaling and thus establishment of antiviral state
CC       in virus infected cells. Induces cell-rounding, cytoskeleton
CC       disorganization and apoptosis in infected cell (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimer. Interacts with viral nucleocapsid (By
CC       similarity). {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q9WH76; P61221: ABCE1; Xeno; NbExp=3; IntAct=EBI-10823897, EBI-2510446;
CC       Q9WH76; O95476: CTDNEP1; Xeno; NbExp=3; IntAct=EBI-10823897, EBI-5323433;
CC       Q9WH76; Q9Y295: DRG1; Xeno; NbExp=3; IntAct=EBI-10823897, EBI-719554;
CC   -!- SUBCELLULAR LOCATION: Virion membrane; Peripheral membrane protein.
CC       Host endomembrane system {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}.
CC   -!- DOMAIN: Late-budding domains (L domains) are short sequence motifs
CC       essential for viral particle budding. They recruit proteins of the host
CC       ESCRT machinery (Endosomal Sorting Complex Required for Transport) or
CC       ESCRT-associated proteins. M contains two overlapping L domains: a PPXY
CC       motif which interacts with the WW domain 3 of NEDD4 and a PTAP/PSAP
CC       motif, which interacts with the UEV domain of TSG101 (By similarity).
CC       {ECO:0000250}.
CC   -!- PTM: Phosphorylated by host. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Most abundant protein in the virion. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the vesiculoviruses matrix protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF128868; AAD25086.1; -; Genomic_RNA.
DR   RefSeq; YP_007641379.1; NC_020805.1.
DR   SMR; Q9WH76; -.
DR   IntAct; Q9WH76; 8.
DR   MINT; Q9WH76; -.
DR   GeneID; 14857909; -.
DR   KEGG; vg:14857909; -.
DR   Proteomes; UP000008448; Genome.
DR   GO; GO:0033645; C:host cell endomembrane system; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:InterPro.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-KW.
DR   GO; GO:0039702; P:viral budding via host ESCRT complex; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.460.10; -; 1.
DR   InterPro; IPR009397; Vesiculo_matrix.
DR   InterPro; IPR036711; VSV_matrix_sf.
DR   Pfam; PF06326; Vesiculo_matrix; 1.
DR   SUPFAM; SSF75404; SSF75404; 1.
PE   1: Evidence at protein level;
KW   Apoptosis; Host membrane; Host-virus interaction; Membrane; Phosphoprotein;
KW   Reference proteome; Viral budding;
KW   Viral budding via the host ESCRT complexes; Viral matrix protein;
KW   Viral release from host cell; Virion.
FT   CHAIN           1..229
FT                   /note="Matrix protein"
FT                   /id="PRO_0000287254"
FT   MOTIF           30..33
FT                   /note="PPXY motif"
FT   MOTIF           42..45
FT                   /note="PTAP/PSAP motif"
SQ   SEQUENCE   229 AA;  26332 MW;  3C71624D865F7A31 CRC64;
     MQRLKKFIAK REKGDKGKMK WNSSMDYDSP PSYQDVRRGI FPTAPLFGME DDMMEFTPSL
     GIQTLKLQYK CVVNINAINP FRDFREAISA MQFWEADYSG YIGKKPFYRA IILHTARQLK
     TSNPGILDRG VVEYHATTQG RALVFHSLGP SPSMMFVPET FTREWNILTN KGTINVKIWL
     GETDTLSELE PILNPVNFRD DREMIEGAAI MGLEIKKQKD NTWLISKSH