ID   MATRX_MPV15             Reviewed;         257 AA.
AC   Q5MKM5; Q50EV7;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   02-DEC-2020, entry version 47.
DE   RecName: Full=Matrix protein;
DE   AltName: Full=M protein {ECO:0000250|UniProtKB:P0DOE7};
GN   Name=M;
OS   Murine pneumonia virus (strain 15) (MPV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus.
OX   NCBI_TaxID=296738;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9140191; DOI=10.1016/s0168-1702(96)01430-x;
RA   Easton A.J., Chambers P.;
RT   "Nucleotide sequence of the genes encoding the matrix and small hydrophobic
RT   proteins of pneumonia virus of mice.";
RL   Virus Res. 48:27-33(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15604443; DOI=10.1099/vir.0.80315-0;
RA   Thorpe L.C., Easton A.J.;
RT   "Genome sequence of the non-pathogenic strain 15 of pneumonia virus of mice
RT   and comparison with the genome of the pathogenic strain J3666.";
RL   J. Gen. Virol. 86:159-169(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15744580; DOI=10.1007/s11262-004-5631-4;
RA   Krempl C.D., Lamirande E.W., Collins P.L.;
RT   "Complete sequence of the RNA genome of pneumonia virus of mice (PVM).";
RL   Virus Genes 30:237-249(2005).
CC   -!- FUNCTION: Plays a crucial role in virus assembly into filaments and
CC       budding. Early in infection, localizes in the nucleus where it may
CC       inhibit host cell transcription. Later in infection, traffics to the
CC       cytoplasm to associate with inclusion bodies, the site of viral
CC       transcription and replication. During virus assembly and budding, acts
CC       as a bridge between the nucleocapsid and the lipid bilayer.
CC       {ECO:0000250|UniProtKB:P0DOE7}.
CC   -!- SUBUNIT: Forms dimers. Forms higher-order oligomers. Interacts with
CC       glycoprotein G (via N-terminus). Interacts with protein M2-1; this
CC       interaction directs the matrix protein localization to cytoplasmic
CC       inclusions comprising viral proteins L, N, P, and M2-1 and mediates the
CC       matrix protein association with the nucleocapsid.
CC       {ECO:0000250|UniProtKB:P0DOE7}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:P0DOE7}. Host
CC       cytoplasm {ECO:0000250|UniProtKB:P0DOE7}. Host nucleus
CC       {ECO:0000250|UniProtKB:P0DOE7}. Host cell membrane
CC       {ECO:0000250|UniProtKB:P0DOE7}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:P0DOE7}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:P0DOE7}. Note=In the cytoplasm, associates with
CC       inclusion bodies. During bud formation, associates at the inner side of
CC       the plasma membrane of infected cells. {ECO:0000250|UniProtKB:P0DOE7}.
CC   -!- SIMILARITY: Belongs to the pneumovirinae M protein family.
CC       {ECO:0000305}.
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DR   EMBL; AY743910; AAW02837.1; -; Genomic_RNA.
DR   EMBL; AY729016; AAW79178.1; -; Genomic_RNA.
DR   SMR; Q5MKM5; -.
DR   Proteomes; UP000133604; Genome.
DR   Proteomes; UP000147186; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:InterPro.
DR   GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-KW.
DR   GO; GO:0019068; P:virion assembly; IEA:InterPro.
DR   Gene3D; 2.70.20.30; -; 1.
DR   InterPro; IPR005056; Pneu_matrix.
DR   InterPro; IPR043062; Pneu_matrix_N.
DR   Pfam; PF03393; Pneumo_matrix; 1.
PE   3: Inferred from homology;
KW   Host cell membrane; Host cytoplasm; Host membrane; Host nucleus;
KW   Host-virus interaction; Membrane; Reference proteome; Viral matrix protein;
KW   Virion.
FT   CHAIN           1..257
FT                   /note="Matrix protein"
FT                   /id="PRO_0000365790"
FT   MOTIF           193..205
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250|UniProtKB:P0DOE7"
FT   VARIANT         167
FT                   /note="Y -> S"
FT   VARIANT         172
FT                   /note="G -> D"
SQ   SEQUENCE   257 AA;  28309 MW;  27A1A561B360AD8E CRC64;
     MEAYLVEMYH GVPYTAAVQL NLVEKHSANI SLTVWIPMFQ TSLPKNSVMD LLHDVTVICT
     QISTVHGPMI KVDLSSSNAG LATMPRQFLI NAIIALDDWG NMDYEVPVAF DKKSFCVTIL
     KPKNMLYTVP SITPTNRPTH ELIAVCSFHN RVTLKSFNIP VFIRALYIRQ QGLDSVEQAI
     SSDVDHAITT ARVAPYAGLT LVINITSTKG AFKLLKAGSQ ILAELGPYLT QVSLHDVIMN
     WKHTGTSYIL KSSSTSG