MATRX_SENDA
ID MATRX_SENDA Reviewed; 348 AA.
AC Q88266; Q782E6;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 23-FEB-2022, entry version 86.
DE RecName: Full=Matrix protein;
DE Short=M protein;
GN Name=M;
OS Sendai virus (strain Hamamatsu) (SeV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae;
OC Respirovirus.
OX NCBI_TaxID=302271;
OH NCBI_TaxID=10144; Cavia cutleri (Guinea pig).
OH NCBI_TaxID=36483; Cricetidae sp. (Hamster).
OH NCBI_TaxID=10090; Mus musculus (Mouse).
OH NCBI_TaxID=10116; Rattus norvegicus (Rat).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8198441; DOI=10.1007/bf01309773;
RA Sakaguchi T., Fujii Y., Kiyotani K., Sasaki M., Yoshida T.;
RT "A field isolate of Sendai virus: its high virulence to mice and genetic
RT divergence from prototype strains.";
RL Arch. Virol. 135:159-164(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=11210938; DOI=10.1023/a:1008130318633;
RA Fujii Y., Kiyotani K., Yoshida T., Sakaguchi T.;
RT "Conserved and non-conserved regions in the Sendai virus genome: evolution
RT of a gene possessing overlapping reading frames.";
RL Virus Genes 22:47-52(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate E15cl2;
RX PubMed=12163573; DOI=10.1128/jvi.76.17.8540-8547.2002;
RA Fujii Y., Sakaguchi T., Kiyotani K., Huang C., Fukuhara N., Egi Y.,
RA Yoshida T.;
RT "Involvement of the leader sequence in Sendai virus pathogenesis revealed
RT by recovery of a pathogenic field isolate from cDNA.";
RL J. Virol. 76:8540-8547(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate E15cl2, Isolate E30cl2, and Isolate E30M15cl5;
RX PubMed=12416681; DOI=10.1023/a:1020165919672;
RA Fujii Y., Sakaguchi T., Kiyotani K., Huang C., Fukuhara N., Yoshida T.;
RT "Identification of mutations associated with attenuation of virulence of a
RT field Sendai virus isolate by egg passage.";
RL Virus Genes 25:189-193(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate E50cl9;
RA Fujii Y., Kiyotani K., Huang C., Fukuhara N., Egi K., Yoshida T.,
RA Sakaguchi T.;
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP DOMAIN LATE-BUDDING.
RX PubMed=17166905; DOI=10.1128/jvi.02218-06;
RA Irie T., Shimazu Y., Yoshida T., Sakaguchi T.;
RT "The YLDL sequence within Sendai virus M protein is critical for budding of
RT virus-like particles and interacts with Alix/AIP1 independently of C
RT protein.";
RL J. Virol. 81:2263-2273(2007).
CC -!- FUNCTION: Plays a crucial role in virion assembly and budding. Forms a
CC shell at the inner face of the plasma membrane and concentrates the HN
CC and F glycoproteins. Acts as a negative regulator for transcription and
CC replication by sticking to the nucleocapsid. This effect might be
CC regulated by the cytoplasmic interaction with tubulin that dissociates
CC the M protein from the nucleocapsid (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimer. Binds to the cytoplasmic regions of F and HN
CC proteins. Interacts with nucleocapsid. Interacts with human alpha-
CC tubulin and beta-tubulin. Interacts with host ANP32B.
CC {ECO:0000250|UniProtKB:P06446}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host cytoplasm
CC {ECO:0000250}. Host cell membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=During bud
CC formation, associates at the inner side of the plasma membrane of
CC infected cells. {ECO:0000250}.
CC -!- DOMAIN: Late-budding domains (L domains) are short sequence motifs
CC essential for viral particle budding. They recruit proteins of the host
CC ESCRT machinery (Endosomal Sorting Complex Required for Transport) or
CC ESCRT-associated proteins. The matrix protein contains one L domain: a
CC YLDL motif. {ECO:0000269|PubMed:17166905}.
CC -!- PTM: A large portion is phosphorylated in the cytoplasm, but not in
CC virion. However, this phosphorylation is not essential for virus
CC replication (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the morbillivirus/respirovirus/rubulavirus M
CC protein family. {ECO:0000305}.
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DR EMBL; D11446; BAA02010.1; -; Genomic_RNA.
DR EMBL; AB065186; BAC79129.1; -; Genomic_RNA.
DR EMBL; AB065187; BAC07509.1; -; Genomic_RNA.
DR EMBL; AB065188; BAC79137.1; -; Genomic_RNA.
DR EMBL; AB065189; BAC79145.1; -; Genomic_RNA.
DR EMBL; AB039658; BAB20023.1; -; Genomic_RNA.
DR SMR; Q88266; -.
DR Proteomes; UP000007191; Genome.
DR Proteomes; UP000008510; Genome.
DR Proteomes; UP000008857; Genome.
DR Proteomes; UP000180650; Genome.
DR Proteomes; UP000180718; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-KW.
DR GO; GO:0039702; P:viral budding via host ESCRT complex; IEA:UniProtKB-KW.
DR Gene3D; 2.70.20.50; -; 1.
DR Gene3D; 2.70.20.60; -; 1.
DR InterPro; IPR000982; Matrix.
DR InterPro; IPR042539; Matrix_C.
DR InterPro; IPR042540; Matrix_N.
DR Pfam; PF00661; Matrix; 1.
PE 3: Inferred from homology;
KW Host cell membrane; Host cytoplasm; Host membrane; Host-virus interaction;
KW Membrane; Phosphoprotein; Reference proteome; Viral budding;
KW Viral budding via the host ESCRT complexes; Viral matrix protein;
KW Viral release from host cell; Virion.
FT CHAIN 1..348
FT /note="Matrix protein"
FT /id="PRO_0000142771"
FT MOTIF 50..53
FT /note="YLDL motif"
FT MOD_RES 70
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
SQ SEQUENCE 348 AA; 38541 MW; FC187D635B50EDE4 CRC64;
MADIYRFPKF SYEDNGTVEP LPLRTGPDKK AIPHIRIVKV GDPPKHGVRY LDLLLLGFFE
TPKQTASLGS VSDLTEHTGY SICGSGSLPI GVAKYHGSDQ ELLKACTDLR ITVRRTVRAG
EMIVYMVDSI GAPLLPWSGR LRQGMIFNAN KVALAPQCLP VDKDIRFRVV FVNGTSLGAI
TIAKIPKTLA DLALPNSISV NLLVTLKTGI PTEQKGVLPV LDDQGEKKLN FMVHLGLIRR
KVGKIYSVEY CKSKIERMRL IFSLGLIGGI SFHVQVTGTL SKTFMSQLAW KRAVCFPLMD
VNPHMNLVIW AASVEITDVD AVFQPAIPRD FRYYPNVVAK NIGRIRKL
