MAT_CATRO
ID MAT_CATRO Reviewed; 443 AA.
AC Q8GZU0;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 43.
DE RecName: Full=Minovincinine 19-hydroxy-O-acetyltransferase {ECO:0000303|PubMed:11154328, ECO:0000303|PubMed:31009114};
DE EC=2.3.1.- {ECO:0000269|PubMed:11154328, ECO:0000269|PubMed:31009114};
GN Name=MAT {ECO:0000303|PubMed:11154328, ECO:0000303|PubMed:31009114};
OS Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC Catharanthinae; Catharanthus.
OX NCBI_TaxID=4058;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP SPECIFICITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=11154328; DOI=10.1104/pp.125.1.189;
RA Laflamme P., St-Pierre B., De Luca V.;
RT "Molecular and biochemical analysis of a Madagascar periwinkle root-
RT specific minovincinine-19-hydroxy-O-acetyltransferase.";
RL Plant Physiol. 125:189-198(2001).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, INDUCTION BY JASMONIC ACID, SUBCELLULAR
RP LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE SPECIFICITY.
RX PubMed=29438577; DOI=10.1111/tpj.13868;
RA Carqueijeiro I., Duge de Bernonville T., Lanoue A., Dang T.-T.,
RA Teijaro C.N., Paetz C., Billet K., Mosquera A., Oudin A., Besseau S.,
RA Papon N., Glevarec G., Atehortua L., Clastre M., Giglioli-Guivarc'h N.,
RA Schneider B., St-Pierre B., Andrade R.B., O'Connor S.E., Courdavault V.;
RT "A BAHD acyltransferase catalyzing 19-O-acetylation of tabersonine
RT derivatives in roots of Catharanthus roseus enables combinatorial synthesis
RT of monoterpene indole alkaloids.";
RL Plant J. 94:469-484(2018).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=31009114; DOI=10.1111/tpj.14346;
RA Williams D., Qu Y., Simionescu R., De Luca V.;
RT "The assembly of (+)-vincadifformine- and (-)-tabersonine-derived
RT monoterpenoid indole alkaloids in Catharanthus roseus involves separate
RT branch pathways.";
RL Plant J. 99:626-636(2019).
CC -!- FUNCTION: Component of the monoterpenoid indole alkaloids (MIAs, e.g.
CC echitovenine, tabersonine, lochnericine, 19-hydroxytabersonine and
CC horhammericine) biosynthetic pathway; MIAs are used in cancer treatment
CC and other medical applications (PubMed:31009114). Acyltransferase
CC catalyzing the conversion of (+)-minovincinine to (+)-echitovenine
CC (PubMed:31009114, PubMed:29438577, PubMed:11154328).
CC {ECO:0000269|PubMed:11154328, ECO:0000269|PubMed:29438577,
CC ECO:0000269|PubMed:31009114}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(+)-minovincinine + acetyl-CoA = (+)-echitovenine + CoA;
CC Xref=Rhea:RHEA:61080, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:144371, ChEBI:CHEBI:144379;
CC Evidence={ECO:0000269|PubMed:11154328, ECO:0000269|PubMed:29438577,
CC ECO:0000269|PubMed:31009114};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61081;
CC Evidence={ECO:0000269|PubMed:11154328, ECO:0000269|PubMed:31009114};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=6 uM for acetyl-CoA (at pH 7.6 and 30 degrees Celsius)
CC {ECO:0000269|PubMed:29438577};
CC KM=28 uM for (-)-minovincinine (at pH 7.6 and 30 degrees Celsius)
CC {ECO:0000269|PubMed:29438577};
CC KM=21 uM for horhammericine (at pH 7.6 and 30 degrees Celsius)
CC {ECO:0000269|PubMed:29438577};
CC KM=40 uM for 19-hydroxytabersonine (at pH 7.6 and 30 degrees Celsius)
CC {ECO:0000269|PubMed:29438577};
CC KM=3 uM for acetyl-CoA (at pH 7.6 and 37 degrees Celsius)
CC {ECO:0000269|PubMed:11154328};
CC KM=250 uM for deactylvindoline (at pH 7.6 and 37 degrees Celsius)
CC {ECO:0000269|PubMed:11154328};
CC KM=120 uM for horhammericine (at pH 7.6 and 37 degrees Celsius)
CC {ECO:0000269|PubMed:11154328};
CC Vmax=0.014 umol/sec/ug enzyme toward acetyl-CoA (at pH 7.6 and 30
CC degrees Celsius) {ECO:0000269|PubMed:29438577};
CC Vmax=0.001 umol/sec/ug enzyme with (-)-minovincinine as substrate (at
CC pH 7.6 and 30 degrees Celsius) {ECO:0000269|PubMed:29438577};
CC Vmax=0.0001 umol/sec/ug enzyme with horhammericine as substrate (at
CC pH 7.6 and 30 degrees Celsius) {ECO:0000269|PubMed:29438577};
CC Vmax=0.25 umol/sec/ug enzyme with 19-hydroxytabersonine as substrate
CC (at pH 7.6 and 30 degrees Celsius) {ECO:0000269|PubMed:29438577};
CC Vmax=25 pmol/sec/mg enzyme with acetyl-CoA as substrate (at pH 7.6
CC and 37 degrees Celsius) {ECO:0000269|PubMed:11154328};
CC Vmax=31 pmol/sec/mg enzyme with deactylvindoline as substrate (at pH
CC 7.6 and 37 degrees Celsius) {ECO:0000269|PubMed:11154328};
CC Vmax=10 pmol/sec/mg enzyme with horhammericine as substrate (at pH
CC 7.6 and 37 degrees Celsius) {ECO:0000269|PubMed:11154328};
CC -!- PATHWAY: Alkaloid biosynthesis. {ECO:0000269|PubMed:31009114}.
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q9ZTK5}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29438577}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00768, ECO:0000269|PubMed:29438577}.
CC -!- TISSUE SPECIFICITY: Expressed in cortical cells of the root tip,
CC especially in hairy roots, as well as in etiolated seedlings
CC (PubMed:11154328). Mostly expressed in roots, and, at lower levels, in
CC leaves (PubMed:29438577). {ECO:0000269|PubMed:11154328,
CC ECO:0000269|PubMed:29438577}.
CC -!- INDUCTION: Induced by jasmonic acid (MeJA).
CC {ECO:0000269|PubMed:29438577}.
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR EMBL; AF253415; AAO13736.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8GZU0; -.
DR SMR; Q8GZU0; -.
DR KEGG; ag:AAO13736; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0016413; F:O-acetyltransferase activity; IDA:UniProtKB.
DR GO; GO:0035835; P:indole alkaloid biosynthetic process; IDA:UniProtKB.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:UniProtKB.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 1: Evidence at protein level;
KW Acyltransferase; Alkaloid metabolism; Coiled coil; Cytoplasm; Nucleus;
KW Transferase.
FT CHAIN 1..443
FT /note="Minovincinine 19-hydroxy-O-acetyltransferase"
FT /id="PRO_0000448559"
FT COILED 316..343
FT /evidence="ECO:0000255"
FT MOTIF 215..222
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT ACT_SITE 157
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT ACT_SITE 384
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
SQ SEQUENCE 443 AA; 49878 MW; 8465AA952F3CFF5C CRC64;
MDSITMVETE TLSKTLIKPS SPTPQSLSHY NLSYNDQNIY PEYIFAGFFY SNPDGHEIST
IREQLQNSLS KTLVSYYPFA GKVVKNDYIH CNDDGIEFVD VRIHCRMNDI LKPELRSYAS
ELIRPNRSTV GSEDSTALVQ LSHFDCGGVA VAFGISHKVA DAATILSFIK DWAASTCDLS
SSHDVSTPVL VSDSIFPRQD NIICGQFPAS PNCVRKRFLF SPEAIERLKS KAIEFGIEKP
TRVEVLTAFL CRCATVAGKS AAKNNNCGQS LPFAVIQAVN LRPLLELPKN SVGNLISIYF
STIKENDTVN IEQEFTKLVI GELRKAKDKL KNLSQEKLNY VARMQDFANC LKELDISSFF
DMENVDIDAY LFSSWCRFPF YDIDFGLGKP IWVCMFQPHF KNCIILMDYP FGDDYGIEAL
ITLEQEKMPA FENNELLLSF ASN
