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MAVE_LEGPH
ID   MAVE_LEGPH              Reviewed;         208 AA.
AC   Q5ZT21;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Effector protein MavE {ECO:0000305};
GN   Name=mavE {ECO:0000303|PubMed:33563829};
GN   OrderedLocusNames=lpg2344 {ECO:0000312|EMBL:AAU28406.1};
OS   Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC
OS   33152 / DSM 7513).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
OC   Legionellaceae; Legionella.
OX   NCBI_TaxID=272624;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX   PubMed=15448271; DOI=10.1126/science.1099776;
RA   Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G.,
RA   Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V.,
RA   Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S., Georghiou A.,
RA   Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A.,
RA   Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A.,
RA   Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A.,
RA   Russo J.J.;
RT   "The genomic sequence of the accidental pathogen Legionella pneumophila.";
RL   Science 305:1966-1968(2004).
RN   [2] {ECO:0007744|PDB:6PIR}
RP   X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 39-172, FUNCTION, SUBUNIT,
RP   SUBCELLULAR LOCATION, DOMAIN, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP   ASP-64; HIS-68; ASN-77; PRO-78; TYR-80 AND SER-102.
RC   STRAIN=AA100 / Serogroup 1, and Philadelphia 1 / ATCC 33152 / DSM 7513;
RX   PubMed=33563829; DOI=10.1128/mbio.03458-20;
RA   Vaughn B., Voth K., Price C.T., Jones S., Ozanic M., Santic M., Cygler M.,
RA   Abu Kwaik Y.;
RT   "An indispensable role for the MavE effector of Legionella pneumophila in
RT   lysosomal evasion.";
RL   MBio 12:e03458-e03458(2021).
CC   -!- FUNCTION: Virulence effector that is indispensable for endoplasmic
CC       reticulum (ER)-mediated remodeling of the Legionella pneumophila-
CC       containing vacuole (LCV) and lysosomal evasion (PubMed:33563829).
CC       Essential for intracellular replication in human monocyte-derived
CC       macrophages (hMDMs) and amoebae, as well as for intrapulmonary
CC       proliferation in mice (PubMed:33563829). {ECO:0000269|PubMed:33563829}.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:33563829}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33563829}. Host
CC       vacuole, host pathogen-containing vacuole, host pathogen-containing
CC       vacuole membrane {ECO:0000269|PubMed:33563829}; Single-pass membrane
CC       protein {ECO:0000255}. Note=Translocated into the host cell via the
CC       type IV secretion system (T4SS) (Probable). During infection, localizes
CC       to the poles of the LCV membrane (PubMed:33563829).
CC       {ECO:0000269|PubMed:33563829, ECO:0000305|PubMed:33563829}.
CC   -!- DOMAIN: Contains a C-terminal transmembrane helix, three copies of
CC       tyrosine-based sorting motifs, and an NPxY eukaryotic motif, which
CC       binds phosphotyrosine-binding domains present on signaling and adapter
CC       eukaryotic proteins (PubMed:33563829). The NPxY eukaryotic motif is
CC       essential for ER-mediated remodeling and lysosomal evasion by the LCV
CC       (PubMed:33563829). {ECO:0000269|PubMed:33563829}.
CC   -!- DISRUPTION PHENOTYPE: The null mutant fails to remodel the LCV with
CC       endoplasmic reticulum (ER)-derived vesicles and is trafficked to the
CC       lysosomes where it is degraded (PubMed:33563829). Mutant is defective
CC       for intracellular growth in hMDMs, amoeba and for intrapulmonary
CC       proliferation in mice (PubMed:33563829). {ECO:0000269|PubMed:33563829}.
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DR   EMBL; AE017354; AAU28406.1; -; Genomic_DNA.
DR   RefSeq; WP_010948050.1; NC_002942.5.
DR   RefSeq; YP_096353.1; NC_002942.5.
DR   PDB; 6PIR; X-ray; 1.65 A; A/B/C=39-172.
DR   PDBsum; 6PIR; -.
DR   SMR; Q5ZT21; -.
DR   PaxDb; Q5ZT21; -.
DR   EnsemblBacteria; AAU28406; AAU28406; lpg2344.
DR   GeneID; 66491471; -.
DR   KEGG; lpn:lpg2344; -.
DR   PATRIC; fig|272624.6.peg.2462; -.
DR   eggNOG; ENOG5030QDH; Bacteria.
DR   HOGENOM; CLU_1561027_0_0_6; -.
DR   OMA; FMRTIIM; -.
DR   Proteomes; UP000000609; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Host membrane; Membrane; Reference proteome; Secreted;
KW   Transmembrane; Transmembrane helix; Virulence.
FT   CHAIN           1..208
FT                   /note="Effector protein MavE"
FT                   /id="PRO_0000456174"
FT   TRANSMEM        184..204
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           77..80
FT                   /note="NPxY eukaryotic motif"
FT                   /evidence="ECO:0000305|PubMed:33563829"
FT   MUTAGEN         64
FT                   /note="D->A: Results in attenuation of L.pneumophila in
FT                   both hMDMs and amoeba. Vacuoles cannot be remodeled by the
FT                   ER and are fused to the lysosomes, leading to bacterial
FT                   degradation."
FT                   /evidence="ECO:0000269|PubMed:33563829"
FT   MUTAGEN         68
FT                   /note="H->A: Results in attenuation of L.pneumophila in
FT                   amoeba."
FT                   /evidence="ECO:0000269|PubMed:33563829"
FT   MUTAGEN         77
FT                   /note="N->A: Does not significantly impede L.pneumophila
FT                   replication within hMDMs."
FT                   /evidence="ECO:0000269|PubMed:33563829"
FT   MUTAGEN         78
FT                   /note="P->A: Results in attenuation of L.pneumophila in
FT                   both hMDMs and amoeba. Vacuoles cannot be remodeled by the
FT                   ER and are fused to the lysosomes, leading to bacterial
FT                   degradation."
FT                   /evidence="ECO:0000269|PubMed:33563829"
FT   MUTAGEN         80
FT                   /note="Y->A: Does not significantly impede L.pneumophila
FT                   replication within hMDMs."
FT                   /evidence="ECO:0000269|PubMed:33563829"
FT   MUTAGEN         102
FT                   /note="S->A: Does not show attenuation."
FT                   /evidence="ECO:0000269|PubMed:33563829"
SQ   SEQUENCE   208 AA;  23928 MW;  CF8C3895A42D7952 CRC64;
     MTRFIMLSFV TGYRKSISCN RFSRNVVSPY FFMRTIIMTR FERNFLINSL MFLETILSVD
     KKLDDAIHHF TQGQYENPRY QINSRITNAD DWSKEDKLKF TSAIAEAIAL VSEKYENPTS
     ETTEQIQSAR NILLDNYVPL LTANTDPENR LKSVRENSSQ IRKELIAKLK DEVPYKSQFE
     NPYVLFPFVA ATVAVAATAA SVLFGNKP
 
 
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