MAVE_LEGPH
ID MAVE_LEGPH Reviewed; 208 AA.
AC Q5ZT21;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Effector protein MavE {ECO:0000305};
GN Name=mavE {ECO:0000303|PubMed:33563829};
GN OrderedLocusNames=lpg2344 {ECO:0000312|EMBL:AAU28406.1};
OS Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC
OS 33152 / DSM 7513).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
OC Legionellaceae; Legionella.
OX NCBI_TaxID=272624;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=15448271; DOI=10.1126/science.1099776;
RA Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G.,
RA Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V.,
RA Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S., Georghiou A.,
RA Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A.,
RA Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A.,
RA Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A.,
RA Russo J.J.;
RT "The genomic sequence of the accidental pathogen Legionella pneumophila.";
RL Science 305:1966-1968(2004).
RN [2] {ECO:0007744|PDB:6PIR}
RP X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 39-172, FUNCTION, SUBUNIT,
RP SUBCELLULAR LOCATION, DOMAIN, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP ASP-64; HIS-68; ASN-77; PRO-78; TYR-80 AND SER-102.
RC STRAIN=AA100 / Serogroup 1, and Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=33563829; DOI=10.1128/mbio.03458-20;
RA Vaughn B., Voth K., Price C.T., Jones S., Ozanic M., Santic M., Cygler M.,
RA Abu Kwaik Y.;
RT "An indispensable role for the MavE effector of Legionella pneumophila in
RT lysosomal evasion.";
RL MBio 12:e03458-e03458(2021).
CC -!- FUNCTION: Virulence effector that is indispensable for endoplasmic
CC reticulum (ER)-mediated remodeling of the Legionella pneumophila-
CC containing vacuole (LCV) and lysosomal evasion (PubMed:33563829).
CC Essential for intracellular replication in human monocyte-derived
CC macrophages (hMDMs) and amoebae, as well as for intrapulmonary
CC proliferation in mice (PubMed:33563829). {ECO:0000269|PubMed:33563829}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:33563829}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33563829}. Host
CC vacuole, host pathogen-containing vacuole, host pathogen-containing
CC vacuole membrane {ECO:0000269|PubMed:33563829}; Single-pass membrane
CC protein {ECO:0000255}. Note=Translocated into the host cell via the
CC type IV secretion system (T4SS) (Probable). During infection, localizes
CC to the poles of the LCV membrane (PubMed:33563829).
CC {ECO:0000269|PubMed:33563829, ECO:0000305|PubMed:33563829}.
CC -!- DOMAIN: Contains a C-terminal transmembrane helix, three copies of
CC tyrosine-based sorting motifs, and an NPxY eukaryotic motif, which
CC binds phosphotyrosine-binding domains present on signaling and adapter
CC eukaryotic proteins (PubMed:33563829). The NPxY eukaryotic motif is
CC essential for ER-mediated remodeling and lysosomal evasion by the LCV
CC (PubMed:33563829). {ECO:0000269|PubMed:33563829}.
CC -!- DISRUPTION PHENOTYPE: The null mutant fails to remodel the LCV with
CC endoplasmic reticulum (ER)-derived vesicles and is trafficked to the
CC lysosomes where it is degraded (PubMed:33563829). Mutant is defective
CC for intracellular growth in hMDMs, amoeba and for intrapulmonary
CC proliferation in mice (PubMed:33563829). {ECO:0000269|PubMed:33563829}.
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DR EMBL; AE017354; AAU28406.1; -; Genomic_DNA.
DR RefSeq; WP_010948050.1; NC_002942.5.
DR RefSeq; YP_096353.1; NC_002942.5.
DR PDB; 6PIR; X-ray; 1.65 A; A/B/C=39-172.
DR PDBsum; 6PIR; -.
DR SMR; Q5ZT21; -.
DR PaxDb; Q5ZT21; -.
DR EnsemblBacteria; AAU28406; AAU28406; lpg2344.
DR GeneID; 66491471; -.
DR KEGG; lpn:lpg2344; -.
DR PATRIC; fig|272624.6.peg.2462; -.
DR eggNOG; ENOG5030QDH; Bacteria.
DR HOGENOM; CLU_1561027_0_0_6; -.
DR OMA; FMRTIIM; -.
DR Proteomes; UP000000609; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Host membrane; Membrane; Reference proteome; Secreted;
KW Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..208
FT /note="Effector protein MavE"
FT /id="PRO_0000456174"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 77..80
FT /note="NPxY eukaryotic motif"
FT /evidence="ECO:0000305|PubMed:33563829"
FT MUTAGEN 64
FT /note="D->A: Results in attenuation of L.pneumophila in
FT both hMDMs and amoeba. Vacuoles cannot be remodeled by the
FT ER and are fused to the lysosomes, leading to bacterial
FT degradation."
FT /evidence="ECO:0000269|PubMed:33563829"
FT MUTAGEN 68
FT /note="H->A: Results in attenuation of L.pneumophila in
FT amoeba."
FT /evidence="ECO:0000269|PubMed:33563829"
FT MUTAGEN 77
FT /note="N->A: Does not significantly impede L.pneumophila
FT replication within hMDMs."
FT /evidence="ECO:0000269|PubMed:33563829"
FT MUTAGEN 78
FT /note="P->A: Results in attenuation of L.pneumophila in
FT both hMDMs and amoeba. Vacuoles cannot be remodeled by the
FT ER and are fused to the lysosomes, leading to bacterial
FT degradation."
FT /evidence="ECO:0000269|PubMed:33563829"
FT MUTAGEN 80
FT /note="Y->A: Does not significantly impede L.pneumophila
FT replication within hMDMs."
FT /evidence="ECO:0000269|PubMed:33563829"
FT MUTAGEN 102
FT /note="S->A: Does not show attenuation."
FT /evidence="ECO:0000269|PubMed:33563829"
SQ SEQUENCE 208 AA; 23928 MW; CF8C3895A42D7952 CRC64;
MTRFIMLSFV TGYRKSISCN RFSRNVVSPY FFMRTIIMTR FERNFLINSL MFLETILSVD
KKLDDAIHHF TQGQYENPRY QINSRITNAD DWSKEDKLKF TSAIAEAIAL VSEKYENPTS
ETTEQIQSAR NILLDNYVPL LTANTDPENR LKSVRENSSQ IRKELIAKLK DEVPYKSQFE
NPYVLFPFVA ATVAVAATAA SVLFGNKP
