ID   MAXSA_BOMMX             Reviewed;         235 AA.
AC   Q5GC94;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Maximins-S type A;
DE   Contains:
DE     RecName: Full=Maximin-S1;
DE   Contains:
DE     RecName: Full=Maximin-S2;
DE   Contains:
DE     RecName: Full=Maximin-S3;
DE   Contains:
DE     RecName: Full=Maximin-S4;
DE   Contains:
DE     RecName: Full=Maximin-S5;
DE   Flags: Precursor;
OS   Bombina maxima (Giant fire-bellied toad) (Chinese red belly toad).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Bombinatoridae; Bombina.
OX   NCBI_TaxID=161274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF MAXIMIN-S1, SYNTHESIS OF
RP   MAXIMIN-S4, AMIDATION AT ASN-83; ASN-118; ASN-153; LYS-188 AND LYS-223, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Skin, and Skin secretion;
RX   PubMed=15649437; DOI=10.1016/j.bbrc.2004.12.094;
RA   Wang T., Zhang J., Shen J.-H., Jin Y., Lee W.-H., Zhang Y.;
RT   "Maximins S, a novel group of antimicrobial peptides from toad Bombina
RT   maxima.";
RL   Biochem. Biophys. Res. Commun. 327:945-951(2005).
CC   -!- FUNCTION: Maximin-S1 has no antimicrobial activity. Has no hemolytic
CC       activity.
CC   -!- FUNCTION: Maximin-S2 has an activity against mycoplasma but has no
CC       activity against common Gram-positive and Gram-negative bacteria nor
CC       fungi. Has no hemolytic activity (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: Maximin-S3 has an activity against mycoplasma but has no
CC       activity against common Gram-positive and Gram-negative bacteria nor
CC       fungi. Has no hemolytic activity (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: Maximin-S4 has an activity against mycoplasma but has no
CC       activity against common Gram-positive and Gram-negative bacteria nor
CC       fungi. Has no hemolytic activity.
CC   -!- FUNCTION: Maximin-S5 has an activity against mycoplasma but has no
CC       activity against common Gram-positive and Gram-negative bacteria nor
CC       fungi. Has no hemolytic activity (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin dorsal glands.
CC   -!- MASS SPECTROMETRY: [Maximin-S2]: Mass=1973.92; Method=MALDI;
CC       Note=Maximin-S2.; Evidence={ECO:0000269|PubMed:15649437};
CC   -!- MASS SPECTROMETRY: [Maximin-S3]: Mass=1959.22; Method=MALDI;
CC       Note=Maximin-S3.; Evidence={ECO:0000269|PubMed:15649437};
CC   -!- MASS SPECTROMETRY: [Maximin-S4]: Mass=2087.90; Method=MALDI;
CC       Note=Maximin-S4.; Evidence={ECO:0000269|PubMed:15649437};
CC   -!- MASS SPECTROMETRY: [Maximin-S5]: Mass=1987.08; Method=MALDI;
CC       Note=Maximin-S5.; Evidence={ECO:0000269|PubMed:15649437};
CC   -!- SIMILARITY: Belongs to the maximin-S family. {ECO:0000305}.
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DR   EMBL; AY652771; AAV97979.1; -; mRNA.
DR   AlphaFoldDB; Q5GC94; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   PROPEP          19..35
FT                   /id="PRO_0000010255"
FT   PEPTIDE         36..49
FT                   /note="Maximin-S1"
FT                   /id="PRO_0000010256"
FT   PROPEP          52..65
FT                   /id="PRO_0000010257"
FT   PEPTIDE         66..83
FT                   /note="Maximin-S2"
FT                   /id="PRO_0000010258"
FT   PROPEP          87..100
FT                   /id="PRO_0000010259"
FT   PEPTIDE         101..118
FT                   /note="Maximin-S3"
FT                   /id="PRO_0000010260"
FT   PROPEP          122..135
FT                   /id="PRO_0000010261"
FT   PEPTIDE         136..153
FT                   /note="Maximin-S3"
FT                   /id="PRO_0000010262"
FT   PROPEP          157..170
FT                   /id="PRO_0000010263"
FT   PEPTIDE         171..188
FT                   /note="Maximin-S4"
FT                   /id="PRO_0000010264"
FT   PROPEP          192..205
FT                   /id="PRO_0000010265"
FT   PEPTIDE         206..223
FT                   /note="Maximin-S5"
FT                   /id="PRO_0000010266"
FT   PROPEP          227..235
FT                   /id="PRO_0000010267"
FT   MOD_RES         83
FT                   /note="Asparagine amide"
FT                   /evidence="ECO:0000269|PubMed:15649437"
FT   MOD_RES         118
FT                   /note="Asparagine amide"
FT                   /evidence="ECO:0000269|PubMed:15649437"
FT   MOD_RES         153
FT                   /note="Asparagine amide"
FT                   /evidence="ECO:0000269|PubMed:15649437"
FT   MOD_RES         188
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000269|PubMed:15649437"
FT   MOD_RES         223
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000269|PubMed:15649437"
SQ   SEQUENCE   235 AA;  26475 MW;  7CE4191F198DDF74 CRC64;
     MNFNYFILVL FFITSGHAKS ETRDVHQEAE NHIKRGSNTG FNFKTLDKEK RSAEEQNLAE
     DLVKRGSNKG FNFMVDMIQA LSNGKRSAEE QDLAEDLVTR GSNKGFNFMV DMINALSNGK
     RSAEEQDLAE DLVKRGSNKG FNFMVDMINA LSNGKRSAEE QDLAEDLVTR RSNKGFNFMV
     DMIQALSKGK RSAEDQDLAE DLVTRGSNKG FNFMVDMIQA LSKGKRSAEQ EKDMK