ID   MAZE3_MYCTU             Reviewed;         106 AA.
AC   O53451; L0T5V0;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Antitoxin MazE3 {ECO:0000305};
GN   Name=mazE3; Synonyms=mazE-mt6 {ECO:0000303|PubMed:16611633};
GN   OrderedLocusNames=Rv1103c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION, AND POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
RN   [3]
RP   GENE NAME, AND POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=16611633; DOI=10.1074/jbc.m512693200;
RA   Zhu L., Zhang Y., Teh J.S., Zhang J., Connell N., Rubin H., Inouye M.;
RT   "Characterization of mRNA interferases from Mycobacterium tuberculosis.";
RL   J. Biol. Chem. 281:18638-18643(2006).
RN   [4]
RP   EXPRESSION IN E.COLI, AND FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=19016878; DOI=10.1111/j.1574-6968.2008.01400.x;
RA   Gupta A.;
RT   "Killing activity and rescue function of genome-wide toxin-antitoxin loci
RT   of Mycobacterium tuberculosis.";
RL   FEMS Microbiol. Lett. 290:45-53(2009).
RN   [5]
RP   EXPRESSION IN M.SMEGMATIS, AND FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY, EXPRESSION IN E.COLI, EXPRESSION IN
RP   M.SMEGMATIS, FUNCTION AS AN ANTITOXIN, AND SUBUNIT.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20705052; DOI=10.1016/j.bbrc.2010.08.023;
RA   Han J.S., Lee J.J., Anandan T., Zeng M., Sripathi S., Jahng W.J., Lee S.H.,
RA   Suh J.W., Kang C.M.;
RT   "Characterization of a chromosomal toxin-antitoxin, Rv1102c-Rv1103c system
RT   in Mycobacterium tuberculosis.";
RL   Biochem. Biophys. Res. Commun. 400:293-298(2010).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [8]
RP   FUNCTION.
RC   STRAIN=H37Rv;
RX   PubMed=23650345; DOI=10.1073/pnas.1222031110;
RA   Schifano J.M., Edifor R., Sharp J.D., Ouyang M., Konkimalla A.,
RA   Husson R.N., Woychik N.A.;
RT   "Mycobacterial toxin MazF-mt6 inhibits translation through cleavage of 23S
RT   rRNA at the ribosomal A site.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:8501-8506(2013).
RN   [9]
RP   INTERACTION WITH MAZF3, AND INDUCTION.
RC   STRAIN=H37Rv;
RX   PubMed=25608501; DOI=10.1038/ncomms7059;
RA   Tiwari P., Arora G., Singh M., Kidwai S., Narayan O.P., Singh R.;
RT   "MazF ribonucleases promote Mycobacterium tuberculosis drug tolerance and
RT   virulence in guinea pigs.";
RL   Nat. Commun. 6:6059-6059(2015).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in E.coli and M.smegmatis neutralizes the effect of
CC       cognate toxin MazF3. Overexpression of MazE3 alone decreased persister
CC       cells formation in M.smegmatis upon challenge with gentamicin or
CC       kanamycin. {ECO:0000269|PubMed:19016878, ECO:0000269|PubMed:20011113,
CC       ECO:0000269|PubMed:20705052, ECO:0000269|PubMed:23650345}.
CC   -!- SUBUNIT: Forms a complex with cognate toxin MazF3, possibly with 1:1
CC       stoichiometry. {ECO:0000269|PubMed:20705052,
CC       ECO:0000269|PubMed:25608501}.
CC   -!- INDUCTION: Mildly induced (about 4-fold) when grown in a non-
CC       replicating state. {ECO:0000269|PubMed:25608501}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP43856.1; -; Genomic_DNA.
DR   PIR; E70897; E70897.
DR   RefSeq; NP_215619.1; NC_000962.3.
DR   RefSeq; WP_003405836.1; NZ_NVQJ01000021.1.
DR   AlphaFoldDB; O53451; -.
DR   SMR; O53451; -.
DR   STRING; 83332.Rv1103c; -.
DR   PaxDb; O53451; -.
DR   DNASU; 885941; -.
DR   GeneID; 45425079; -.
DR   GeneID; 885941; -.
DR   KEGG; mtu:Rv1103c; -.
DR   TubercuList; Rv1103c; -.
DR   eggNOG; ENOG50339Z8; Bacteria.
DR   OMA; PHDDVNT; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0097351; F:toxin sequestering activity; IPI:MTBBASE.
DR   GO; GO:0098754; P:detoxification; IMP:MTBBASE.
DR   GO; GO:0045926; P:negative regulation of growth; IMP:MTBBASE.
PE   1: Evidence at protein level;
KW   Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..106
FT                   /note="Antitoxin MazE3"
FT                   /id="PRO_0000406298"
SQ   SEQUENCE   106 AA;  11395 MW;  837F8B4BDAAC1C7C CRC64;
     MYLPWGVVLA GGANGFGAGA YQTGTICEVS TQIAVRLPDE IVAFIDDEVR GQHARSRAAV
     VLRALERERR RRLAERDAEI LATNTSATGD LDTLAGHCAR TALDID