ID   MAZE4_MYCTU             Reviewed;         100 AA.
AC   P9WJ91; L0T8F3; P0A5E9; P71775;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Probable antitoxin MazE4 {ECO:0000305};
GN   Name=mazE4; Synonyms=mazE-mt7 {ECO:0000303|PubMed:16611633};
GN   OrderedLocusNames=Rv1494; ORFNames=MTCY277.16;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   GENE NAME, AND POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=16611633; DOI=10.1074/jbc.m512693200;
RA   Zhu L., Zhang Y., Teh J.S., Zhang J., Connell N., Rubin H., Inouye M.;
RT   "Characterization of mRNA interferases from Mycobacterium tuberculosis.";
RL   J. Biol. Chem. 281:18638-18643(2006).
RN   [3]
RP   EXPRESSION IN M.SMEGMATIS, AND FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20724443; DOI=10.1093/nar/gkq737;
RA   Huang F., He Z.G.;
RT   "Characterization of an interplay between a Mycobacterium tuberculosis MazF
RT   homolog, Rv1495 and its sole DNA topoisomerase I.";
RL   Nucleic Acids Res. 38:8219-8230(2010).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4,
CC       its cognate toxin, on growth. {ECO:0000269|PubMed:20724443}.
CC   -!- SUBUNIT: Forms a complex with cognate toxin MazF4.
CC       {ECO:0000250|UniProtKB:O53451}.
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DR   EMBL; AL123456; CCP44255.1; -; Genomic_DNA.
DR   PIR; A70712; A70712.
DR   RefSeq; NP_216010.1; NC_000962.3.
DR   RefSeq; WP_003900349.1; NZ_NVQJ01000004.1.
DR   PDB; 5XE3; X-ray; 2.30 A; E/F=19-99.
DR   PDBsum; 5XE3; -.
DR   AlphaFoldDB; P9WJ91; -.
DR   SMR; P9WJ91; -.
DR   STRING; 83332.Rv1494; -.
DR   PaxDb; P9WJ91; -.
DR   GeneID; 45425474; -.
DR   GeneID; 886502; -.
DR   KEGG; mtu:Rv1494; -.
DR   TubercuList; Rv1494; -.
DR   eggNOG; ENOG5032MNZ; Bacteria.
DR   OMA; RAWRNAR; -.
DR   Proteomes; UP000001584; Chromosome.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..100
FT                   /note="Probable antitoxin MazE4"
FT                   /id="PRO_0000103858"
FT   REGION          77..100
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           25..36
FT                   /evidence="ECO:0007829|PDB:5XE3"
FT   HELIX           42..72
FT                   /evidence="ECO:0007829|PDB:5XE3"
FT   TURN            74..77
FT                   /evidence="ECO:0007829|PDB:5XE3"
FT   STRAND          78..80
FT                   /evidence="ECO:0007829|PDB:5XE3"
FT   HELIX           81..98
FT                   /evidence="ECO:0007829|PDB:5XE3"
SQ   SEQUENCE   100 AA;  11205 MW;  6AC437A0F88DA20D CRC64;
     MPFLVALSGI ISGVRDHSMT VRLDQQTRQR LQDIVKGGYR SANAAIVDAI NKRWEALHDE
     QLDAAYAAAI HDNPAYPYES EAERSAARAR RNARQQRSAQ