ID   MAZE7_MYCTU             Reviewed;          77 AA.
AC   P9WJ85; L0TA24; Q79FI0; Q8VJR2;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Antitoxin MazE7 {ECO:0000305};
GN   Name=mazE7; OrderedLocusNames=Rv2063;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION, AND POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
RN   [3]
RP   EXPRESSION IN E.COLI, AND FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=19016878; DOI=10.1111/j.1574-6968.2008.01400.x;
RA   Gupta A.;
RT   "Killing activity and rescue function of genome-wide toxin-antitoxin loci
RT   of Mycobacterium tuberculosis.";
RL   FEMS Microbiol. Lett. 290:45-53(2009).
RN   [4]
RP   EXPRESSION IN M.SMEGMATIS, AND LACK OF FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in E.coli but not in M.smegmatis neutralizes the effect
CC       of cognate toxin MazF7. {ECO:0000269|PubMed:19016878}.
CC   -!- SUBUNIT: Forms a complex with cognate toxin MazF7.
CC       {ECO:0000250|UniProtKB:O53451}.
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DR   EMBL; AL123456; CCP44836.1; -; Genomic_DNA.
DR   RefSeq; WP_003410651.1; NZ_NVQJ01000047.1.
DR   RefSeq; YP_177657.1; NC_000962.3.
DR   PDB; 6A6X; X-ray; 2.70 A; C/D=1-77.
DR   PDBsum; 6A6X; -.
DR   AlphaFoldDB; P9WJ85; -.
DR   SMR; P9WJ85; -.
DR   STRING; 83332.Rv2063; -.
DR   PaxDb; P9WJ85; -.
DR   DNASU; 3205116; -.
DR   GeneID; 3205116; -.
DR   KEGG; mtu:Rv2063; -.
DR   TubercuList; Rv2063; -.
DR   OMA; HRDWDIT; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0098754; P:detoxification; IMP:MTBBASE.
DR   GO; GO:0045927; P:positive regulation of growth; IMP:MTBBASE.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..77
FT                   /note="Antitoxin MazE7"
FT                   /id="PRO_0000406301"
FT   REGION          49..77
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        49..71
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          4..10
FT                   /evidence="ECO:0007829|PDB:6A6X"
FT   HELIX           11..23
FT                   /evidence="ECO:0007829|PDB:6A6X"
FT   HELIX           28..57
FT                   /evidence="ECO:0007829|PDB:6A6X"
FT   HELIX           59..68
FT                   /evidence="ECO:0007829|PDB:6A6X"
FT   HELIX           69..73
FT                   /evidence="ECO:0007829|PDB:6A6X"
SQ   SEQUENCE   77 AA;  8450 MW;  A9EF5C8343CD86DE CRC64;
     MSTSTTIRVS TQTRDRLAAQ ARERGISMSA LLTELAAQAE RQAIFRAERE ASHAETTTQA
     VRDEDREWEG TVGDGLG