ID   MAZE9_MYCTU             Reviewed;          76 AA.
AC   P0CL61; L0TAX2;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-APR-2011, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Antitoxin MazE9 {ECO:0000305};
GN   Name=mazE9; Synonyms=mazE-mt1 {ECO:0000303|PubMed:16611633};
GN   OrderedLocusNames=Rv2801A;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION, AND POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
RN   [3]
RP   GENE NAME, AND POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=16611633; DOI=10.1074/jbc.m512693200;
RA   Zhu L., Zhang Y., Teh J.S., Zhang J., Connell N., Rubin H., Inouye M.;
RT   "Characterization of mRNA interferases from Mycobacterium tuberculosis.";
RL   J. Biol. Chem. 281:18638-18643(2006).
RN   [4]
RP   EXPRESSION IN E.COLI, AND FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=19016878; DOI=10.1111/j.1574-6968.2008.01400.x;
RA   Gupta A.;
RT   "Killing activity and rescue function of genome-wide toxin-antitoxin loci
RT   of Mycobacterium tuberculosis.";
RL   FEMS Microbiol. Lett. 290:45-53(2009).
RN   [5]
RP   EXPRESSION IN M.SMEGMATIS, AND FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [6]
RP   FUNCTION AS AN ANTITOXIN, AND INTERACTION WITH TOXIN MAZF9.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20876537; DOI=10.1074/jbc.m110.163105;
RA   Zhu L., Sharp J.D., Kobayashi H., Woychik N.A., Inouye M.;
RT   "Noncognate Mycobacterium tuberculosis toxin-antitoxins can physically and
RT   functionally interact.";
RL   J. Biol. Chem. 285:39732-39738(2010).
RN   [7]
RP   INTERACTION WITH MAZF9, AND INDUCTION.
RC   STRAIN=H37Rv;
RX   PubMed=25608501; DOI=10.1038/ncomms7059;
RA   Tiwari P., Arora G., Singh M., Kidwai S., Narayan O.P., Singh R.;
RT   "MazF ribonucleases promote Mycobacterium tuberculosis drug tolerance and
RT   virulence in guinea pigs.";
RL   Nat. Commun. 6:6059-6059(2015).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in E.coli and M.smegmatis neutralizes the effect of
CC       cognate toxin MazF9. {ECO:0000269|PubMed:19016878,
CC       ECO:0000269|PubMed:20011113, ECO:0000269|PubMed:20876537}.
CC   -!- SUBUNIT: Forms a complex with cognate toxin MazF9.
CC       {ECO:0000269|PubMed:20876537, ECO:0000269|PubMed:25608501}.
CC   -!- INDUCTION: Mildy induced (5-fold) when grown in a non-replicating
CC       state. {ECO:0000269|PubMed:25608501}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP45601.1; -; Genomic_DNA.
DR   RefSeq; WP_003901465.1; NZ_NVQJ01000020.1.
DR   RefSeq; YP_007411507.1; NC_000962.3.
DR   PDB; 6KYT; X-ray; 2.00 A; C/F/P/Q=1-76.
DR   PDBsum; 6KYT; -.
DR   AlphaFoldDB; P0CL61; -.
DR   SMR; P0CL61; -.
DR   STRING; 83332.Rv2801A; -.
DR   GeneID; 14515892; -.
DR   KEGG; mtu:Rv2801A; -.
DR   TubercuList; Rv2801A; -.
DR   eggNOG; COG3609; Bacteria.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0097351; F:toxin sequestering activity; IPI:MTBBASE.
DR   GO; GO:0098754; P:detoxification; IMP:MTBBASE.
DR   GO; GO:0045927; P:positive regulation of growth; IMP:MTBBASE.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR002145; CopG.
DR   Pfam; PF01402; RHH_1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..76
FT                   /note="Antitoxin MazE9"
FT                   /id="PRO_0000406303"
FT   STRAND          3..7
FT                   /evidence="ECO:0007829|PDB:6KYT"
FT   HELIX           9..22
FT                   /evidence="ECO:0007829|PDB:6KYT"
FT   HELIX           27..39
FT                   /evidence="ECO:0007829|PDB:6KYT"
FT   HELIX           43..56
FT                   /evidence="ECO:0007829|PDB:6KYT"
FT   HELIX           59..63
FT                   /evidence="ECO:0007829|PDB:6KYT"
FT   HELIX           64..66
FT                   /evidence="ECO:0007829|PDB:6KYT"
FT   TURN            67..70
FT                   /evidence="ECO:0007829|PDB:6KYT"
SQ   SEQUENCE   76 AA;  8263 MW;  43F2BD228F7BC1A9 CRC64;
     MKLSVSLSDD DVAILDAYVK RAGLPSRSAG LQHAIRVLRY PTLEDDYANA WQEWSAAGDT
     DAWEQTVGDG VGDAPR