ID   MAZE_MYCS2              Reviewed;          69 AA.
AC   A0R0N3;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Antitoxin MazE {ECO:0000303|PubMed:22199354};
GN   Name=mazE; OrderedLocusNames=MSMEG_4447, MSMEI_4337;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION AS AN ANTITOXIN, FUNCTION IN TRANSCRIPTION REGULATION, INDUCTION,
RP   AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=22199354; DOI=10.1074/jbc.m111.286856;
RA   Frampton R., Aggio R.B., Villas-Boas S.G., Arcus V.L., Cook G.M.;
RT   "Toxin-antitoxin systems of Mycobacterium smegmatis are essential for cell
RT   survival.";
RL   J. Biol. Chem. 287:5340-5356(2012).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Neutralizes the effect of cognate toxin MazF. In M.smegmatis 3 TA
CC       systems (VapB-VapC, MazE-MazF and Phd-Doc) may be involved in
CC       monitoring the nutritional supply and physiological state of the cell,
CC       linking catabolic with anabolic reactions.
CC       {ECO:0000269|PubMed:22199354}.
CC   -!- SUBUNIT: Forms a complex with cognate toxin MazF.
CC       {ECO:0000250|UniProtKB:O53451}.
CC   -!- INDUCTION: Constitutively expressed during exponential growth and early
CC       stationary phase, it decreases in late stationary phase; part of the
CC       mazE-mazF operon. Negatively autoregulated by one or both of MazE-MazF.
CC       {ECO:0000269|PubMed:22199354}.
CC   -!- DISRUPTION PHENOTYPE: The mazE-mazF operon is not essential. A triple
CC       TA mutant (missing vapB-vapC, mazE-mazF and phd-doc TA systems)
CC       survives antibiotic challenge, suggesting the TA systems are not
CC       required to generate drug-resistant cells. However the mutant is more
CC       sensitive to oxidative and heat stress, and does not survive long term
CC       starvation during aerobic growth on complex medium. There is a
CC       difference in the level of branched-chain amino acids, which may play a
CC       role in monitoring the nutritional supply and physiological state of
CC       the cell. {ECO:0000269|PubMed:22199354}.
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DR   EMBL; CP000480; ABK71290.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP40793.1; -; Genomic_DNA.
DR   RefSeq; WP_011729843.1; NZ_SIJM01000026.1.
DR   RefSeq; YP_888721.1; NC_008596.1.
DR   AlphaFoldDB; A0R0N3; -.
DR   STRING; 246196.MSMEI_4337; -.
DR   EnsemblBacteria; ABK71290; ABK71290; MSMEG_4447.
DR   EnsemblBacteria; AFP40793; AFP40793; MSMEI_4337.
DR   GeneID; 66735780; -.
DR   KEGG; msg:MSMEI_4337; -.
DR   KEGG; msm:MSMEG_4447; -.
DR   PATRIC; fig|246196.19.peg.4357; -.
DR   eggNOG; ENOG50330YX; Bacteria.
DR   OMA; MAHVNSR; -.
DR   OrthoDB; 2048811at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   InterPro; IPR021558; MazE-like.
DR   Pfam; PF11455; DUF3018; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Repressor; Toxin-antitoxin system; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..69
FT                   /note="Antitoxin MazE"
FT                   /id="PRO_0000420847"
SQ   SEQUENCE   69 AA;  8115 MW;  E946BFDFA79E7440 CRC64;
     MTPARDRVRR HRERLRRQGL RPVQIWVPDV NAPEFRREAH RQSELVAAGE HEAEDQAFVD
     AISVDWDDA