ID   MAZE_STAAE              Reviewed;          56 AA.
AC   P0C7B4;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Antitoxin MazE;
GN   Name=mazE; OrderedLocusNames=NWMN_1974.1;
OS   Staphylococcus aureus (strain Newman).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=426430;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Newman;
RX   PubMed=17951380; DOI=10.1128/jb.01000-07;
RA   Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.;
RT   "Genome sequence of Staphylococcus aureus strain Newman and comparative
RT   analysis of staphylococcal genomes: polymorphism and evolution of two major
RT   pathogenicity islands.";
RL   J. Bacteriol. 190:300-310(2008).
RN   [2]
RP   FUNCTION AS AN ANTITOXIN, INDUCTION, AND SUBUNIT.
RC   STRAIN=Newman;
RX   PubMed=17933891; DOI=10.1128/jb.01272-07;
RA   Fu Z., Donegan N.P., Memmi G., Cheung A.L.;
RT   "Characterization of mazFSa, an endoribonuclease from Staphylococcus
RT   aureus.";
RL   J. Bacteriol. 189:8871-8879(2007).
RN   [3]
RP   FUNCTION.
RC   STRAIN=Newman;
RX   PubMed=19168622; DOI=10.1128/jb.00907-08;
RA   Fu Z., Tamber S., Memmi G., Donegan N.P., Cheung A.L.;
RT   "Overexpression of MazFsa in Staphylococcus aureus induces bacteriostasis
RT   by selectively targeting mRNAs for cleavage.";
RL   J. Bacteriol. 191:2051-2059(2009).
RN   [4]
RP   FUNCTION.
RX   PubMed=19251861; DOI=10.1128/jb.01815-08;
RA   Zhu L., Inoue K., Yoshizumi S., Kobayashi H., Zhang Y., Ouyang M., Kato F.,
RA   Sugai M., Inouye M.;
RT   "Staphylococcus aureus MazF specifically cleaves a pentad sequence, UACAU,
RT   which is unusually abundant in the mRNA for pathogenic adhesive factor
RT   SraP.";
RL   J. Bacteriol. 191:3248-3255(2009).
RN   [5]
RP   FUNCTION.
RC   STRAIN=NRS26;
RX   PubMed=23994560; DOI=10.1016/j.ab.2013.08.018;
RA   van Rensburg J.J., Hergenrother P.J.;
RT   "Detection of endogenous MazF enzymatic activity in Staphylococcus
RT   aureus.";
RL   Anal. Biochem. 443:81-87(2013).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Labile antitoxin that binds to cognate MazF toxin and counteracts its
CC       endoribonuclease activity. {ECO:0000269|PubMed:17933891,
CC       ECO:0000269|PubMed:19168622, ECO:0000269|PubMed:19251861,
CC       ECO:0000269|PubMed:23994560}.
CC   -!- SUBUNIT: Forms a complex with cognate toxin MazF which inhibits the
CC       endoribonuclease activity of MazF. {ECO:0000269|PubMed:17933891}.
CC   -!- INTERACTION:
CC       P0C7B4; A6QIR4: mazF; NbExp=2; IntAct=EBI-6469965, EBI-6469950;
CC   -!- INDUCTION: By heat shock and by exposure to sub-MIC concentrations of
CC       several antimicrobial agents such as doxycycline, erythromycin and
CC       penicillin. {ECO:0000269|PubMed:17933891}.
CC   -!- SIMILARITY: Belongs to the MazE/EndoAI family. {ECO:0000305}.
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DR   EMBL; AP009351; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; WP_000948331.1; NZ_CP023390.1.
DR   AlphaFoldDB; P0C7B4; -.
DR   SMR; P0C7B4; -.
DR   IntAct; P0C7B4; 1.
DR   Proteomes; UP000006386; Chromosome.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
PE   1: Evidence at protein level;
KW   Toxin-antitoxin system.
FT   CHAIN           1..56
FT                   /note="Antitoxin MazE"
FT                   /id="PRO_0000330719"
SQ   SEQUENCE   56 AA;  6252 MW;  B6DA0AFB386D7691 CRC64;
     MLSFSQNRSH SLEQSLKEGY SQMADLNLSL ANEAFPIECE ACDCNETYLS SNSTNE