ID   MAZF1_MYCBO             Reviewed;         105 AA.
AC   P64860; A0A1R3XYI1; P71776; X2BIK0;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Endoribonuclease MazF1 {ECO:0000305};
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin MazF1;
DE   AltName: Full=mRNA interferase MazF1;
GN   Name=mazF1; OrderedLocusNames=BQ2027_MB1532;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Acts as an endoribonuclease on single-strand RNA, cleaving between the
CC       first and second bases in the sequence UCGCU. Neutralized by
CC       coexpression with cognate antitoxin MazE1.
CC       {ECO:0000250|UniProtKB:P9WII5}.
CC   -!- SUBUNIT: Forms a complex with cognate antitoxin MazE1.
CC       {ECO:0000250|UniProtKB:P9WII5}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; LT708304; SIU00135.1; -; Genomic_DNA.
DR   RefSeq; NP_855184.1; NC_002945.3.
DR   RefSeq; WP_003407593.1; NC_002945.4.
DR   AlphaFoldDB; P64860; -.
DR   SMR; P64860; -.
DR   EnsemblBacteria; SIU00135; SIU00135; BQ2027_MB1532.
DR   GeneID; 45425475; -.
DR   PATRIC; fig|233413.5.peg.1674; -.
DR   OMA; GYANADN; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..105
FT                   /note="Endoribonuclease MazF1"
FT                   /id="PRO_0000201903"
SQ   SEQUENCE   105 AA;  11410 MW;  52F4C417DD07EB16 CRC64;
     MNAPLRGQVY RCDLGYGAKP WLIVSNNARN RHTADVVAVR LTTTRRTIPT WVAMGPSDPL
     TGYVNADNIE TLGKDELGDY LGEVTPATMN KINTALATAL GLPWP