ID   MAZF2_MYCBO             Reviewed;         114 AA.
AC   P64912; A0A1R3XZY5; Q10867; X2BJU0;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Endoribonuclease MazF2 {ECO:0000305};
DE            EC=3.1.27.- {ECO:0000305};
DE   AltName: Full=Toxin MazF2;
DE   AltName: Full=mRNA interferase MazF2;
GN   Name=mazF2; OrderedLocusNames=BQ2027_MB2014C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Acts as an endoribonuclease on single-strand RNA, cleaving between the
CC       second and third bases in the sequences CUCCU and UUCCU. Neutralized by
CC       coexpression with cognate antitoxin MazE2.
CC       {ECO:0000250|UniProtKB:P9WII3}.
CC   -!- SUBUNIT: Probably forms a complex with cognate antitoxin MazE2.
CC       {ECO:0000250|UniProtKB:P9WII3}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; LT708304; SIU00620.1; -; Genomic_DNA.
DR   RefSeq; NP_855664.1; NC_002945.3.
DR   RefSeq; WP_003410010.1; NC_002945.4.
DR   AlphaFoldDB; P64912; -.
DR   SMR; P64912; -.
DR   EnsemblBacteria; SIU00620; SIU00620; BQ2027_MB2014C.
DR   GeneID; 45425969; -.
DR   PATRIC; fig|233413.5.peg.2212; -.
DR   OMA; VFNHSRI; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
DR   PIRSF; PIRSF033490; MazF; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..114
FT                   /note="Endoribonuclease MazF2"
FT                   /id="PRO_0000201905"
SQ   SEQUENCE   114 AA;  12262 MW;  59A18C54B9EDDBCE CRC64;
     MVISRAEIYW ADLGPPSGSQ PAKRRPVLVI QSDPYNASRL ATVIAAVITS NTALAAMPGN
     VFLPATTTRL PRDSVVNVTA IVTLNKTDLT DRVGEVPASL MHEVDRGLRR VLDL