ID   MAZF2_MYCTU             Reviewed;         102 AA.
AC   P9WII1; L0T7C6; O06780; Q7D9G9;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Probable endoribonuclease MazF2 {ECO:0000305};
DE            EC=3.1.-.-;
DE   AltName: Full=Probable toxin MazF-mt4 {ECO:0000303|PubMed:16611633};
GN   Name=mazF2; Synonyms=mazF-mt4 {ECO:0000303|PubMed:16611633};
GN   OrderedLocusNames=Rv0659c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   EXPRESSION IN E.COLI.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=16611633; DOI=10.1074/jbc.m512693200;
RA   Zhu L., Zhang Y., Teh J.S., Zhang J., Connell N., Rubin H., Inouye M.;
RT   "Characterization of mRNA interferases from Mycobacterium tuberculosis.";
RL   J. Biol. Chem. 281:18638-18643(2006).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in E.coli partially inhibits cell growth after one cell
CC       generation (PubMed:16611633). Its cognate antitoxin is MazE2. Probably
CC       an endoribonuclease (By similarity). {ECO:0000250|UniProtKB:P9WIH9,
CC       ECO:0000269|PubMed:16611633, ECO:0000305}.
CC   -!- SUBUNIT: Forms a complex with cognate antitoxin MazE2.
CC       {ECO:0000250|UniProtKB:P9WIH9}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43402.1; -; Genomic_DNA.
DR   PIR; F70534; F70534.
DR   RefSeq; NP_215173.1; NC_000962.3.
DR   RefSeq; WP_003403376.1; NZ_NVQJ01000007.1.
DR   AlphaFoldDB; P9WII1; -.
DR   SMR; P9WII1; -.
DR   STRING; 83332.Rv0659c; -.
DR   PaxDb; P9WII1; -.
DR   PRIDE; P9WII1; -.
DR   DNASU; 888134; -.
DR   GeneID; 888134; -.
DR   KEGG; mtu:Rv0659c; -.
DR   TubercuList; Rv0659c; -.
DR   eggNOG; COG2337; Bacteria.
DR   OMA; RRHEICR; -.
DR   PhylomeDB; P9WII1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0006402; P:mRNA catabolic process; IBA:GO_Central.
DR   GO; GO:0016075; P:rRNA catabolic process; IBA:GO_Central.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..102
FT                   /note="Probable endoribonuclease MazF2"
FT                   /id="PRO_0000406305"
SQ   SEQUENCE   102 AA;  11320 MW;  6EB2F795CAAE1E11 CRC64;
     MRRGELWFAA TPGGDRPVLV LTRDPVADRI GAVVVVALTR TRRGLVSELE LTAVENRVPS
     DCVVNFDNIH TLPRTAFRRR ITRLSPARLH EACQTLRAST GC