ID   MAZF4_MYCTO             Reviewed;         105 AA.
AC   P9WII4; L0T6U0; P64859; P71776;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Endoribonuclease MazF4 {ECO:0000305};
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin MazF4;
DE   AltName: Full=mRNA interferase MazF4;
GN   Name=mazF4; OrderedLocusNames=MT1542;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Acts as an endoribonuclease on single-strand RNA, cleaving between the
CC       first and second bases in the sequence UCGCU. Neutralized by cognate
CC       antitoxin MazE4. {ECO:0000250|UniProtKB:P9WII5}.
CC   -!- SUBUNIT: Forms a complex with cognate antitoxin MazE4.
CC       {ECO:0000250|UniProtKB:P9WII5}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK45809.1; -; Genomic_DNA.
DR   PIR; B70712; B70712.
DR   RefSeq; WP_003407593.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WII4; -.
DR   SMR; P9WII4; -.
DR   EnsemblBacteria; AAK45809; AAK45809; MT1542.
DR   GeneID; 45425475; -.
DR   KEGG; mtc:MT1542; -.
DR   PATRIC; fig|83331.31.peg.1659; -.
DR   HOGENOM; CLU_176190_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..105
FT                   /note="Endoribonuclease MazF4"
FT                   /id="PRO_0000428001"
SQ   SEQUENCE   105 AA;  11410 MW;  52F4C417DD07EB16 CRC64;
     MNAPLRGQVY RCDLGYGAKP WLIVSNNARN RHTADVVAVR LTTTRRTIPT WVAMGPSDPL
     TGYVNADNIE TLGKDELGDY LGEVTPATMN KINTALATAL GLPWP