ID   MAZF5_MYCTU             Reviewed;         109 AA.
AC   P95272; L0TB16;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 4.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Probable endoribonuclease MazF5 {ECO:0000305};
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin MazF5 {ECO:0000303|PubMed:20011113};
GN   Name=mazF5; Synonyms=mazF-mt5 {ECO:0000303|PubMed:16611633};
GN   OrderedLocusNames=Rv1942c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   GENE NAME, AND POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=16611633; DOI=10.1074/jbc.m512693200;
RA   Zhu L., Zhang Y., Teh J.S., Zhang J., Connell N., Rubin H., Inouye M.;
RT   "Characterization of mRNA interferases from Mycobacterium tuberculosis.";
RL   J. Biol. Chem. 281:18638-18643(2006).
RN   [3]
RP   INDUCTION.
RX   PubMed=19389781; DOI=10.1099/mic.0.027086-0;
RA   de la Paz Santangelo M., Klepp L., Nunez-Garcia J., Blanco F.C., Soria M.,
RA   Garcia-Pelayo M.C., Bianco M.V., Cataldi A.A., Golby P., Jackson M.,
RA   Gordon S.V., Bigi F.;
RT   "Mce3R, a TetR-type transcriptional repressor, controls the expression of a
RT   regulon involved in lipid metabolism in Mycobacterium tuberculosis.";
RL   Microbiology 155:2245-2255(2009).
RN   [4]
RP   EXPRESSION IN M.SMEGMATIS, AND FUNCTION AS A TOXIN.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in M.smegmatis inhibits colony formation. Its toxic
CC       effect is neutralized by coexpression with cognate antitoxin MazE5
CC       (PubMed:20011113). Probably an endoribonuclease (By similarity).
CC       {ECO:0000250|UniProtKB:P9WIH9, ECO:0000269|PubMed:20011113}.
CC   -!- SUBUNIT: Forms a complex with cognate antitoxin MazE5.
CC       {ECO:0000250|UniProtKB:P9WIH9}.
CC   -!- INDUCTION: Repressed by Mce3R. {ECO:0000269|PubMed:19389781}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP44709.1; -; Genomic_DNA.
DR   PIR; B70637; B70637.
DR   RefSeq; NP_216458.1; NC_000962.3.
DR   RefSeq; WP_003409778.1; NZ_NVQJ01000034.1.
DR   AlphaFoldDB; P95272; -.
DR   SMR; P95272; -.
DR   STRING; 83332.Rv1942c; -.
DR   PaxDb; P95272; -.
DR   DNASU; 885606; -.
DR   GeneID; 45425914; -.
DR   GeneID; 885606; -.
DR   KEGG; mtu:Rv1942c; -.
DR   TubercuList; Rv1942c; -.
DR   eggNOG; COG2337; Bacteria.
DR   OMA; MRQICGA; -.
DR   PhylomeDB; P95272; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0006402; P:mRNA catabolic process; IBA:GO_Central.
DR   GO; GO:0045926; P:negative regulation of growth; IMP:MTBBASE.
DR   GO; GO:0016075; P:rRNA catabolic process; IBA:GO_Central.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..109
FT                   /note="Probable endoribonuclease MazF5"
FT                   /id="PRO_0000406307"
SQ   SEQUENCE   109 AA;  11820 MW;  2105D696B4F5ECF1 CRC64;
     MTALPARGEV WWCEMAEIGR RPVVVLSRDA AIPRLRRALV APCTTTIRGL ASEVVLEPGS
     DPIPRRSAVN LDSVESVSVA VLVNRLGRLA DIRMRAICTA LEVAVDCSR