MAZF7_MYCTU
ID MAZF7_MYCTU Reviewed; 136 AA.
AC P0CL62; L0T8I4;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Probable endoribonuclease MazF7 {ECO:0000305};
DE EC=3.1.-.-;
DE AltName: Full=Toxin MazF7 {ECO:0000303|PubMed:20011113};
GN Name=mazF7; OrderedLocusNames=Rv2063A;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION, AND POSSIBLE FUNCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=15718296; DOI=10.1093/nar/gki201;
RA Pandey D.P., Gerdes K.;
RT "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT host-associated prokaryotes.";
RL Nucleic Acids Res. 33:966-976(2005).
RN [3]
RP EXPRESSION IN E.COLI, AND FUNCTION AS A TOXIN.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=19016878; DOI=10.1111/j.1574-6968.2008.01400.x;
RA Gupta A.;
RT "Killing activity and rescue function of genome-wide toxin-antitoxin loci
RT of Mycobacterium tuberculosis.";
RL FEMS Microbiol. Lett. 290:45-53(2009).
RN [4]
RP EXPRESSION IN M.SMEGMATIS, AND FUNCTION AS A TOXIN.
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA Ramage H.R., Connolly L.E., Cox J.S.;
RT "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT antitoxin systems: implications for pathogenesis, stress responses, and
RT evolution.";
RL PLoS Genet. 5:E1000767-E1000767(2009).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC Upon expression in E.coli and M.smegmatis inhibits cell growth and
CC colony formation. Its toxic effect is neutralized by coexpression with
CC cognate antitoxin MazE7 (PubMed:19016878, PubMed:20011113). Probably an
CC endoribonuclease (By similarity). {ECO:0000250|UniProtKB:P9WIH9,
CC ECO:0000269|PubMed:19016878, ECO:0000269|PubMed:20011113}.
CC -!- SUBUNIT: Forms a complex with cognate antitoxin MazE7.
CC {ECO:0000250|UniProtKB:P9WIH9}.
CC -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR EMBL; AL123456; CCP44837.1; -; Genomic_DNA.
DR RefSeq; WP_003410654.1; NZ_NVQJ01000047.1.
DR RefSeq; YP_004837055.2; NC_000962.3.
DR PDB; 5WYG; X-ray; 2.36 A; A/C=1-136.
DR PDB; 6A6X; X-ray; 2.70 A; A/B=1-136.
DR PDB; 7DU4; X-ray; 2.18 A; A/B=2-136.
DR PDBsum; 5WYG; -.
DR PDBsum; 6A6X; -.
DR PDBsum; 7DU4; -.
DR AlphaFoldDB; P0CL62; -.
DR SMR; P0CL62; -.
DR STRING; 83332.Rv2063A; -.
DR PaxDb; P0CL62; -.
DR GeneID; 14515888; -.
DR KEGG; mtu:Rv2063A; -.
DR TubercuList; Rv2063A; -.
DR eggNOG; COG2337; Bacteria.
DR InParanoid; P0CL62; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004521; F:endoribonuclease activity; IDA:MTBBASE.
DR GO; GO:0044003; P:modulation by symbiont of host process; IMP:MTBBASE.
DR GO; GO:0006402; P:mRNA catabolic process; IBA:GO_Central.
DR GO; GO:0045926; P:negative regulation of growth; IMP:MTBBASE.
DR GO; GO:0017148; P:negative regulation of translation; IDA:MTBBASE.
DR GO; GO:0016075; P:rRNA catabolic process; IBA:GO_Central.
DR Gene3D; 2.30.30.110; -; 1.
DR InterPro; IPR003477; PemK-like.
DR InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR PANTHER; PTHR33988; PTHR33988; 1.
DR Pfam; PF02452; PemK_toxin; 1.
DR PIRSF; PIRSF033490; MazF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW Toxin-antitoxin system.
FT CHAIN 1..136
FT /note="Probable endoribonuclease MazF7"
FT /id="PRO_0000406308"
FT REGION 115..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 1..3
FT /evidence="ECO:0007829|PDB:6A6X"
FT STRAND 8..13
FT /evidence="ECO:0007829|PDB:7DU4"
FT STRAND 25..30
FT /evidence="ECO:0007829|PDB:7DU4"
FT HELIX 34..36
FT /evidence="ECO:0007829|PDB:7DU4"
FT STRAND 44..54
FT /evidence="ECO:0007829|PDB:7DU4"
FT HELIX 65..67
FT /evidence="ECO:0007829|PDB:7DU4"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:7DU4"
FT HELIX 77..79
FT /evidence="ECO:0007829|PDB:7DU4"
FT STRAND 81..84
FT /evidence="ECO:0007829|PDB:7DU4"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:7DU4"
FT STRAND 88..94
FT /evidence="ECO:0007829|PDB:7DU4"
FT HELIX 97..110
FT /evidence="ECO:0007829|PDB:7DU4"
SQ SEQUENCE 136 AA; 14235 MW; 36A1BD37858279F3 CRC64;
MAEPRRGDLW LVSLGAARAG EPGKHRPAVV VSVDELLTGI DDELVVVVPV SSSRSRTPLR
PPVAPSEGVA ADSVAVCRGV RAVARARLVE RLGALKPATM RAIENALTLI LGLPTGPERG
EAATHSPVRW TGGRDP
