ID   MAZF_ECO57              Reviewed;         111 AA.
AC   P0AE71; P33645;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Endoribonuclease MazF;
DE            EC=3.1.27.-;
DE   AltName: Full=Toxin MazF;
DE   AltName: Full=mRNA interferase MazF;
GN   Name=mazF; Synonyms=chpA; OrderedLocusNames=Z4097, ECs3642;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       MazF is a sequence-specific endoribonuclease that inhibits protein
CC       synthesis and induces bacterial stasis. It is very stable, single-
CC       strand specific and cleavage is independent of the ribosome. The
CC       endoribonuclease activity (a toxin) is inhibited by the labile cognate
CC       antitoxin MazE. Toxicity results when the levels of MazE decrease in
CC       the cell, leading to mRNA degradation. Both MazE and MazE-MazF bind to
CC       the promoter region of the mazE-mazF operon to inhibit their
CC       transcription. {ECO:0000250|UniProtKB:P0AE70}.
CC   -!- SUBUNIT: Probably a dimer. Forms a heterohexamer composed of
CC       alternating toxin and antitoxin homodimers MazF(2)-MazE(2)-MazF(2).
CC       {ECO:0000250|UniProtKB:P0AE70}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG57895.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37065.1; -; Genomic_DNA.
DR   PIR; B91084; B91084.
DR   PIR; C85929; C85929.
DR   RefSeq; NP_311669.1; NC_002695.1.
DR   RefSeq; WP_000254738.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AE71; -.
DR   BMRB; P0AE71; -.
DR   SMR; P0AE71; -.
DR   STRING; 155864.EDL933_3960; -.
DR   EnsemblBacteria; AAG57895; AAG57895; Z4097.
DR   EnsemblBacteria; BAB37065; BAB37065; ECs_3642.
DR   GeneID; 58459985; -.
DR   GeneID; 916551; -.
DR   KEGG; ece:Z4097; -.
DR   KEGG; ecs:ECs_3642; -.
DR   PATRIC; fig|386585.9.peg.3806; -.
DR   eggNOG; COG2337; Bacteria.
DR   HOGENOM; CLU_121823_2_3_6; -.
DR   OMA; QIKGYPF; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Repressor; RNA-binding; Toxin-antitoxin system; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..111
FT                   /note="Endoribonuclease MazF"
FT                   /id="PRO_0000201898"
SQ   SEQUENCE   111 AA;  12098 MW;  1579C867DD6B96AC CRC64;
     MVSRYVPDMG DLIWVDFDPT KGSEQAGHRP AVVLSPFMYN NKTGMCLCVP CTTQSKGYPF
     EVVLSGQERD GVALADQVKS IAWRARGATK KGTVAPEELQ LIKAKINVLI G