ID   MAZF_MYCS2              Reviewed;         109 AA.
AC   A0R0N4;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Probable endoribonuclease MazF {ECO:0000305};
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin MazF {ECO:0000303|PubMed:22199354};
GN   Name=mazF; OrderedLocusNames=MSMEG_4448, MSMEI_4338;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION AS A TOXIN, FUNCTION IN TRANSCRIPTION REGULATION, INDUCTION, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=22199354; DOI=10.1074/jbc.m111.286856;
RA   Frampton R., Aggio R.B., Villas-Boas S.G., Arcus V.L., Cook G.M.;
RT   "Toxin-antitoxin systems of Mycobacterium smegmatis are essential for cell
RT   survival.";
RL   J. Biol. Chem. 287:5340-5356(2012).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Upon overexpression inhibits cell growth and colony formation. Its
CC       toxic effect is neutralized by coexpression with cognate antitoxin
CC       MazE. In M.smegmatis 3 TA systems (VapB-VapC, MazE-MazF and Phd-Doc)
CC       may be involved in monitoring the nutritional supply and physiological
CC       state of the cell, linking catabolic with anabolic reactions
CC       (PubMed:22199354). Probably an endoribonuclease (By similarity).
CC       {ECO:0000250|UniProtKB:P9WIH9, ECO:0000269|PubMed:22199354}.
CC   -!- SUBUNIT: Forms a complex with cognate antitoxin MazE.
CC       {ECO:0000250|UniProtKB:P9WIH9}.
CC   -!- INDUCTION: Constitutively expressed during exponential growth and early
CC       stationary phase, it decreases in late stationary phase; part of the
CC       mazE-mazF operon. Negatively autoregulated by one or both of MazE-MazF.
CC       {ECO:0000269|PubMed:22199354}.
CC   -!- DISRUPTION PHENOTYPE: The mazE-mazF operon is not essential. A triple
CC       TA mutant (missing vapB-vapC, mazE-mazF and phd-doc TA systems)
CC       survives antibiotic challenge, suggesting the TA systems are not
CC       required to generate drug-resistant cells. However the mutant is more
CC       sensitive to oxidative and heat stress, and does not survive long term
CC       starvation during aerobic growth on complex medium. There is a
CC       difference in the level of branched-chain amino acids, which may play a
CC       role in monitoring the nutritional supply and physiological state of
CC       the cell. {ECO:0000269|PubMed:22199354}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; CP000480; ABK72484.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP40794.1; -; Genomic_DNA.
DR   RefSeq; WP_003895797.1; NZ_SIJM01000026.1.
DR   RefSeq; YP_888722.1; NC_008596.1.
DR   AlphaFoldDB; A0R0N4; -.
DR   SMR; A0R0N4; -.
DR   STRING; 246196.MSMEI_4338; -.
DR   EnsemblBacteria; ABK72484; ABK72484; MSMEG_4448.
DR   EnsemblBacteria; AFP40794; AFP40794; MSMEI_4338.
DR   GeneID; 66735781; -.
DR   KEGG; msg:MSMEI_4338; -.
DR   KEGG; msm:MSMEG_4448; -.
DR   PATRIC; fig|246196.56.peg.4440; -.
DR   eggNOG; COG2337; Bacteria.
DR   OMA; QKPSQVM; -.
DR   OrthoDB; 1754531at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; Repressor;
KW   Toxin-antitoxin system; Transcription; Transcription regulation.
FT   CHAIN           1..109
FT                   /note="Probable endoribonuclease MazF"
FT                   /id="PRO_0000420844"
SQ   SEQUENCE   109 AA;  11772 MW;  2F2370E19023E997 CRC64;
     MRRGDIYTAA ARGAYTGKPR PVLIIQDDRF DATASVTVVP FTTSDTDAPL LRIRIEPTPA
     TGLSTVSSLM IDKVTTVPRS SLTHRVGRLA ETDMVRTDRA LLVFLGIAG