ID   MAZF_STAA1              Reviewed;         120 AA.
AC   A7X4P5;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Endoribonuclease MazF;
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin MazF;
DE   AltName: Full=mRNA interferase MazF;
GN   Name=mazF; OrderedLocusNames=SAHV_2053;
OS   Staphylococcus aureus (strain Mu3 / ATCC 700698).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=418127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu3 / ATCC 700698;
RX   PubMed=17954695; DOI=10.1128/aac.00534-07;
RA   Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.;
RT   "Mutated response regulator graR is responsible for phenotypic conversion
RT   of Staphylococcus aureus from heterogeneous vancomycin-intermediate
RT   resistance to vancomycin-intermediate resistance.";
RL   Antimicrob. Agents Chemother. 52:45-53(2008).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Ribosome-independent, sequence-specific endoribonuclease that cleaves
CC       mRNA, thus inhibiting protein synthesis and inducing bacterial stasis.
CC       It cuts between the first and nucleotides of 5'-UACAU-3' in single-
CC       stranded RNA. Neutralized by coexpression with cognate antitoxin MazE.
CC       {ECO:0000250|UniProtKB:A6QIR4}.
CC   -!- SUBUNIT: Forms a complex with MazE which is no longer active as an
CC       endoribonuclease. {ECO:0000250|UniProtKB:A6QIR4}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; AP009324; BAF78936.1; -; Genomic_DNA.
DR   RefSeq; WP_000621175.1; NZ_CTYB01000037.1.
DR   AlphaFoldDB; A7X4P5; -.
DR   BMRB; A7X4P5; -.
DR   SMR; A7X4P5; -.
DR   KEGG; saw:SAHV_2053; -.
DR   HOGENOM; CLU_121823_1_0_9; -.
DR   OMA; NDIGNQY; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
DR   PIRSF; PIRSF033490; MazF; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; RNA-binding; Toxin-antitoxin system.
FT   CHAIN           1..120
FT                   /note="Endoribonuclease MazF"
FT                   /id="PRO_0000330698"
SQ   SEQUENCE   120 AA;  13442 MW;  41D531536BAE2B57 CRC64;
     MIRRGDVYLA DLSPVQGSEQ GGVRPVVIIQ NDTGNKYSPT VIVAAITGRI NKAKIPTHVE
     IEKKKYKLDK DSVILLEQIR TLDKKRLKEK LTYLSDDKMK EVDNALMISL GLNAVAHQKN