ID   MAZF_STAA8              Reviewed;         120 AA.
AC   Q2FWI8; O05341;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Endoribonuclease MazF;
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin MazF;
DE   AltName: Full=mRNA interferase MazF;
GN   Name=mazF; OrderedLocusNames=SAOUHSC_02303;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9042755; DOI=10.1007/s002030050428;
RA   Kullik I., Giachino P.;
RT   "The alternative sigma factor sigmaB in Staphylococcus aureus: regulation
RT   of the sigB operon in response to growth phase and heat shock.";
RL   Arch. Microbiol. 167:151-159(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Ribosome-independent, sequence-specific endoribonuclease that cleaves
CC       mRNA, thus inhibiting protein synthesis and inducing bacterial stasis.
CC       It cuts between the first and nucleotides of 5'-UACAU-3' in single-
CC       stranded RNA. Neutralized by coexpression with cognate antitoxin MazE.
CC       {ECO:0000250|UniProtKB:A6QIR4}.
CC   -!- SUBUNIT: Forms a complex with MazE which is no longer active as an
CC       endoribonuclease. {ECO:0000250|UniProtKB:A6QIR4}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Y07645; CAA68928.1; -; Genomic_DNA.
DR   EMBL; CP000253; ABD31337.1; -; Genomic_DNA.
DR   RefSeq; WP_000621175.1; NZ_LS483365.1.
DR   RefSeq; YP_500782.1; NC_007795.1.
DR   PDB; 5DLO; X-ray; 1.40 A; A=2-120.
DR   PDBsum; 5DLO; -.
DR   AlphaFoldDB; Q2FWI8; -.
DR   BMRB; Q2FWI8; -.
DR   SMR; Q2FWI8; -.
DR   STRING; 1280.SAXN108_2313; -.
DR   EnsemblBacteria; ABD31337; ABD31337; SAOUHSC_02303.
DR   GeneID; 3919174; -.
DR   KEGG; sao:SAOUHSC_02303; -.
DR   PATRIC; fig|93061.5.peg.2087; -.
DR   eggNOG; COG2337; Bacteria.
DR   HOGENOM; CLU_121823_1_0_9; -.
DR   OMA; NDIGNQY; -.
DR   PRO; PR:Q2FWI8; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006402; P:mRNA catabolic process; IBA:GO_Central.
DR   GO; GO:0016075; P:rRNA catabolic process; IBA:GO_Central.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
DR   PIRSF; PIRSF033490; MazF; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   RNA-binding; Toxin-antitoxin system.
FT   CHAIN           1..120
FT                   /note="Endoribonuclease MazF"
FT                   /id="PRO_0000330702"
FT   STRAND          6..11
FT                   /evidence="ECO:0007829|PDB:5DLO"
FT   STRAND          22..28
FT                   /evidence="ECO:0007829|PDB:5DLO"
FT   HELIX           32..37
FT                   /evidence="ECO:0007829|PDB:5DLO"
FT   STRAND          39..47
FT                   /evidence="ECO:0007829|PDB:5DLO"
FT   STRAND          58..61
FT                   /evidence="ECO:0007829|PDB:5DLO"
FT   TURN            63..67
FT                   /evidence="ECO:0007829|PDB:5DLO"
FT   STRAND          72..83
FT                   /evidence="ECO:0007829|PDB:5DLO"
FT   HELIX           84..86
FT                   /evidence="ECO:0007829|PDB:5DLO"
FT   STRAND          87..93
FT                   /evidence="ECO:0007829|PDB:5DLO"
FT   HELIX           96..109
FT                   /evidence="ECO:0007829|PDB:5DLO"
SQ   SEQUENCE   120 AA;  13442 MW;  41D531536BAE2B57 CRC64;
     MIRRGDVYLA DLSPVQGSEQ GGVRPVVIIQ NDTGNKYSPT VIVAAITGRI NKAKIPTHVE
     IEKKKYKLDK DSVILLEQIR TLDKKRLKEK LTYLSDDKMK EVDNALMISL GLNAVAHQKN