ID   MAZF_STAAB              Reviewed;         117 AA.
AC   Q2YUI5;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Endoribonuclease MazF;
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin MazF;
DE   AltName: Full=mRNA interferase MazF;
GN   Name=mazF; OrderedLocusNames=SAB1953c;
OS   Staphylococcus aureus (strain bovine RF122 / ET3-1).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=273036;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=bovine RF122 / ET3-1;
RX   PubMed=17971880; DOI=10.1371/journal.pone.0001120;
RA   Herron-Olson L., Fitzgerald J.R., Musser J.M., Kapur V.;
RT   "Molecular correlates of host specialization in Staphylococcus aureus.";
RL   PLoS ONE 2:E1120-E1120(2007).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Ribosome-independent, sequence-specific endoribonuclease that cleaves
CC       mRNA, thus inhibiting protein synthesis and inducing bacterial stasis.
CC       It cuts between the first and nucleotides of 5'-UACAU-3' in single-
CC       stranded RNA. Neutralized by coexpression with cognate antitoxin MazE.
CC       {ECO:0000250|UniProtKB:A6QIR4}.
CC   -!- SUBUNIT: Forms a complex with MazE which is no longer active as an
CC       endoribonuclease. {ECO:0000250|UniProtKB:A6QIR4}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; AJ938182; CAI81642.1; -; Genomic_DNA.
DR   RefSeq; WP_000621174.1; NC_007622.1.
DR   AlphaFoldDB; Q2YUI5; -.
DR   BMRB; Q2YUI5; -.
DR   SMR; Q2YUI5; -.
DR   KEGG; sab:SAB1953c; -.
DR   HOGENOM; CLU_121823_1_0_9; -.
DR   OMA; NDIGNQY; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
DR   PIRSF; PIRSF033490; MazF; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; RNA-binding; Toxin-antitoxin system.
FT   CHAIN           1..117
FT                   /note="Endoribonuclease MazF"
FT                   /id="PRO_0000330694"
SQ   SEQUENCE   117 AA;  13071 MW;  F36BAE2B573EC397 CRC64;
     MIRRGDVYLA DLSPVQGSEQ GGVRPVVIIQ NDTGNKYSPT VIVAAITGRI NKAKIPTHVE
     IEKKKYKLDK DSVILLEQIR TLDKKRLKEK LTYLSDDKMK EVDNALMISL GLNAVAH