MAZF_STAAN
ID MAZF_STAAN Reviewed; 120 AA.
AC Q7A4G9;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Endoribonuclease MazF;
DE EC=3.1.-.-;
DE AltName: Full=SaMazF {ECO:0000303|PubMed:21213075};
DE AltName: Full=Toxin MazF;
DE AltName: Full=mRNA interferase MazF;
GN Name=mazF; OrderedLocusNames=SA1873;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N315;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=N315;
RA Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT aureus strain N315.";
RL Submitted (OCT-2007) to UniProtKB.
RN [3]
RP STRUCTURE BY NMR OF 2-120, AND SUBUNIT.
RX PubMed=21213075; DOI=10.1007/s12104-010-9290-1;
RA Zorzini V., Haesaerts S., Cheung A., Loris R., van Nuland N.A.;
RT "1H, 13C, and 15N backbone and side-chain chemical shift assignment of the
RT staphylococcal MazF mRNA interferase.";
RL Biomol. NMR. Assign. 5:157-160(2011).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 2-120, FUNCTION,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RX PubMed=24748664; DOI=10.1093/nar/gku266;
RA Zorzini V., Buts L., Sleutel M., Garcia-Pino A., Talavera A., Haesaerts S.,
RA De Greve H., Cheung A., van Nuland N.A., Loris R.;
RT "Structural and biophysical characterization of Staphylococcus aureus
RT SaMazF shows conservation of functional dynamics.";
RL Nucleic Acids Res. 42:6709-6725(2014).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system,
CC cannot be expressed in E.coli in the absence of cognate antitoxin MazE
CC (PubMed:24748664). Ribosome-independent, sequence-specific
CC endoribonuclease that cleaves mRNA, thus inhibiting protein synthesis
CC and inducing bacterial stasis; it cuts between the first and
CC nucleotides of 5'-UACAU-3' in single-stranded RNA (By similarity). Its
CC endoribnuclease activity is neutralized by coexpression with cognate
CC antitoxin MazE (PubMed:24748664). {ECO:0000250|UniProtKB:A6QIR4,
CC ECO:0000269|PubMed:24748664}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Temperature dependence:
CC Thermostable at 90 degrees Celsius. {ECO:0000269|PubMed:24748664};
CC -!- SUBUNIT: Homodimer. Forms a complex with MazE which inhibits the
CC endoribonuclease activity of MazF. {ECO:0000269|PubMed:24748664,
CC ECO:0000305|PubMed:21213075}.
CC -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR EMBL; BA000018; BAB43155.1; -; Genomic_DNA.
DR PIR; B89999; B89999.
DR RefSeq; WP_000621175.1; NC_002745.2.
DR PDB; 2MF2; NMR; -; A/B=2-120.
DR PDB; 4MZM; X-ray; 2.10 A; A/B/C/D=2-120.
DR PDB; 4MZP; X-ray; 2.70 A; A/B/C/D/E/F/G/H=2-120.
DR PDB; 4MZT; X-ray; 2.30 A; A/B=2-120.
DR PDBsum; 2MF2; -.
DR PDBsum; 4MZM; -.
DR PDBsum; 4MZP; -.
DR PDBsum; 4MZT; -.
DR AlphaFoldDB; Q7A4G9; -.
DR SMR; Q7A4G9; -.
DR EnsemblBacteria; BAB43155; BAB43155; BAB43155.
DR KEGG; sau:SA1873; -.
DR HOGENOM; CLU_121823_1_0_9; -.
DR OMA; NDIGNQY; -.
DR Proteomes; UP000000751; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR Gene3D; 2.30.30.110; -; 1.
DR InterPro; IPR003477; PemK-like.
DR InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR PANTHER; PTHR33988; PTHR33988; 1.
DR Pfam; PF02452; PemK_toxin; 1.
DR PIRSF; PIRSF033490; MazF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Endonuclease; Hydrolase; Nuclease; RNA-binding;
KW Toxin-antitoxin system.
FT CHAIN 1..120
FT /note="Endoribonuclease MazF"
FT /id="PRO_0000330701"
FT STRAND 6..11
FT /evidence="ECO:0007829|PDB:4MZM"
FT STRAND 22..28
FT /evidence="ECO:0007829|PDB:4MZM"
FT HELIX 32..37
FT /evidence="ECO:0007829|PDB:4MZM"
FT STRAND 39..48
FT /evidence="ECO:0007829|PDB:4MZM"
FT STRAND 58..61
FT /evidence="ECO:0007829|PDB:4MZM"
FT TURN 63..67
FT /evidence="ECO:0007829|PDB:4MZM"
FT STRAND 72..83
FT /evidence="ECO:0007829|PDB:4MZM"
FT HELIX 84..86
FT /evidence="ECO:0007829|PDB:4MZM"
FT STRAND 87..93
FT /evidence="ECO:0007829|PDB:4MZM"
FT HELIX 96..109
FT /evidence="ECO:0007829|PDB:4MZM"
SQ SEQUENCE 120 AA; 13442 MW; 41D531536BAE2B57 CRC64;
MIRRGDVYLA DLSPVQGSEQ GGVRPVVIIQ NDTGNKYSPT VIVAAITGRI NKAKIPTHVE
IEKKKYKLDK DSVILLEQIR TLDKKRLKEK LTYLSDDKMK EVDNALMISL GLNAVAHQKN
