ID   MAZF_STAEP              Reviewed;         120 AA.
AC   Q9F7V5;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Endoribonuclease MazF;
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin MazF;
DE   AltName: Full=mRNA interferase MazF;
GN   Name=mazF;
OS   Staphylococcus epidermidis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1282;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Clinical isolate 1457;
RX   PubMed=11274123; DOI=10.1128/jb.183.8.2624-2633.2001;
RA   Knobloch J.K.-M., Bartscht K., Sabottke A., Rohde H., Feucht H.-H.,
RA   Mack D.;
RT   "Biofilm formation by Staphylococcus epidermidis depends on functional
RT   rsbU, an activator of the sigB operon: differential activation mechanisms
RT   due to ethanol and salt stress.";
RL   J. Bacteriol. 183:2624-2633(2001).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Ribosome-independent, sequence-specific endoribonuclease that cleaves
CC       mRNA, thus inhibiting protein synthesis and inducing bacterial stasis.
CC       It cuts between the first and nucleotides of 5'-UACAU-3' in single-
CC       stranded RNA. Neutralized by coexpression with cognate antitoxin MazE.
CC       {ECO:0000250|UniProtKB:A6QIR4}.
CC   -!- SUBUNIT: Forms a complex with MazE which is no longer active as an
CC       endoribonuclease. {ECO:0000250|UniProtKB:A6QIR4}.
CC   -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR   EMBL; AF274004; AAG23809.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9F7V5; -.
DR   SMR; Q9F7V5; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.30.110; -; 1.
DR   InterPro; IPR003477; PemK-like.
DR   InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR   PANTHER; PTHR33988; PTHR33988; 1.
DR   Pfam; PF02452; PemK_toxin; 1.
DR   PIRSF; PIRSF033490; MazF; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; RNA-binding; Toxin-antitoxin system.
FT   CHAIN           1..120
FT                   /note="Endoribonuclease MazF"
FT                   /id="PRO_0000330705"
SQ   SEQUENCE   120 AA;  13457 MW;  F29FE86F5358AFC3 CRC64;
     MIRRGDVYLA DLSPVQGSEQ GGVRPVVIIQ NDTGNKYSPT VIVAAITDGI NKAKIPTHVE
     IEKKKYKLDK DSVILLEQIR TLDKKRLKEK LTFLSESKMI EVDNALDISL GLNNFDHHKS