MAZF_STAS1
ID MAZF_STAS1 Reviewed; 120 AA.
AC Q49Z22;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Endoribonuclease MazF;
DE EC=3.1.-.-;
DE AltName: Full=Toxin MazF;
DE AltName: Full=mRNA interferase MazF;
GN Name=mazF; OrderedLocusNames=SSP0809;
OS Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS 20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=342451;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT pathogenesis of uncomplicated urinary tract infection.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC Ribosome-independent, sequence-specific endoribonuclease that cleaves
CC mRNA, thus inhibiting protein synthesis and inducing bacterial stasis.
CC It cuts between the first and nucleotides of 5'-UACAU-3' in single-
CC stranded RNA. Neutralized by coexpression with cognate antitoxin MazE.
CC {ECO:0000250|UniProtKB:A6QIR4}.
CC -!- SUBUNIT: Forms a complex with MazE which is no longer active as an
CC endoribonuclease. {ECO:0000250|UniProtKB:A6QIR4}.
CC -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR EMBL; AP008934; BAE17954.1; -; Genomic_DNA.
DR RefSeq; WP_011302703.1; NZ_MTGA01000032.1.
DR AlphaFoldDB; Q49Z22; -.
DR SMR; Q49Z22; -.
DR STRING; 342451.SSP0809; -.
DR EnsemblBacteria; BAE17954; BAE17954; SSP0809.
DR KEGG; ssp:SSP0809; -.
DR PATRIC; fig|342451.11.peg.811; -.
DR eggNOG; COG2337; Bacteria.
DR HOGENOM; CLU_121823_1_0_9; -.
DR OMA; NDIGNQY; -.
DR OrthoDB; 1754531at2; -.
DR Proteomes; UP000006371; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR Gene3D; 2.30.30.110; -; 1.
DR InterPro; IPR003477; PemK-like.
DR InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR PANTHER; PTHR33988; PTHR33988; 1.
DR Pfam; PF02452; PemK_toxin; 1.
DR PIRSF; PIRSF033490; MazF; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Nuclease; Reference proteome; RNA-binding;
KW Toxin-antitoxin system.
FT CHAIN 1..120
FT /note="Endoribonuclease MazF"
FT /id="PRO_0000330709"
SQ SEQUENCE 120 AA; 13483 MW; 99BCE08B812C3ECF CRC64;
MMRRGDVYLA DLSPVQGSEQ GGIRPVVIIQ NDTGNKYSPT VIVAAITGRI NKAKIPTHVE
IEKSKYKLDK DSVILLEQIR TLDKNRLKEK LTYLSDKKMK EVNNAIDISL GLHTIRSHKA