MB101_ARATH
ID MB101_ARATH Reviewed; 490 AA.
AC O80883; A8MSC3; C0Z3H7; Q8W1W5;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 178.
DE RecName: Full=Transcription factor MYB101 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 101 {ECO:0000303|PubMed:11597504};
DE Short=AtM1 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB101 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Protein ABNORMAL SHOOT 7 {ECO:0000303|PubMed:25268707};
GN Name=MYB101 {ECO:0000303|PubMed:11597504};
GN Synonyms=ABS7 {ECO:0000303|PubMed:25268707};
GN OrderedLocusNames=At2g32460 {ECO:0000312|Araport:AT2G32460};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=11743113; DOI=10.1104/pp.010442;
RA Gocal G.F.W., Sheldon C.C., Gubler F., Moritz T., Bagnall D.J.,
RA MacMillan C.P., Li S.F., Parish R.W., Dennis E.S., Weigel D., King R.W.;
RT "GAMYB-like genes, flowering, and gibberellin signaling in Arabidopsis.";
RL Plant Physiol. 127:1682-1693(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: A genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [10]
RP REPRESSION BY MICRORNA159.
RX PubMed=15226253; DOI=10.1242/dev.01206;
RA Achard P., Herr A., Baulcombe D.C., Harberd N.P.;
RT "Modulation of floral development by a gibberellin-regulated microRNA.";
RL Development 131:3357-3365(2004).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, AND REPRESSION BY MICRORNA159.
RC STRAIN=cv. Columbia;
RX PubMed=17217461; DOI=10.1111/j.1365-313x.2006.02980.x;
RA Reyes J.L., Chua N.-H.;
RT "ABA induction of miR159 controls transcript levels of two MYB factors
RT during Arabidopsis seed germination.";
RL Plant J. 49:592-606(2007).
RN [12]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY GIBBERELLIC ACID.
RC STRAIN=cv. Columbia;
RX PubMed=20699403; DOI=10.1104/pp.110.160630;
RA Alonso-Peral M.M., Li J., Li Y., Allen R.S., Schnippenkoetter W., Ohms S.,
RA White R.G., Millar A.A.;
RT "The microRNA159-regulated GAMYB-like genes inhibit growth and promote
RT programmed cell death in Arabidopsis.";
RL Plant Physiol. 154:757-771(2010).
RN [13]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22101548; DOI=10.1007/s00497-011-0178-8;
RA Renak D., Dupl'akova N., Honys D.;
RT "Wide-scale screening of T-DNA lines for transcription factor genes
RT affecting male gametophyte development in Arabidopsis.";
RL Sex. Plant Reprod. 25:39-60(2012).
RN [14]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=23791732; DOI=10.1016/j.cub.2013.05.021;
RA Leydon A.R., Beale K.M., Woroniecka K., Castner E., Chen J., Horgan C.,
RA Palanivelu R., Johnson M.A.;
RT "Three MYB transcription factors control pollen tube differentiation
RT required for sperm release.";
RL Curr. Biol. 23:1209-1214(2013).
RN [15]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=24278028; DOI=10.1371/journal.pgen.1003933;
RA Liang Y., Tan Z.-M., Zhu L., Niu Q.-K., Zhou J.-J., Li M., Chen L.-Q.,
RA Zhang X.-Q., Ye D.;
RT "MYB97, MYB101 and MYB120 function as male factors that control pollen
RT tube-synergid interaction in Arabidopsis thaliana fertilization.";
RL PLoS Genet. 9:E1003933-E1003933(2013).
RN [16]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=25268707; DOI=10.1371/journal.pone.0107637;
RA An R., Liu X., Wang R., Wu H., Liang S., Shao J., Qi Y., An L., Yu F.;
RT "The over-expression of two transcription factors, ABS5/bHLH30 and
RT ABS7/MYB101, leads to upwardly curly leaves.";
RL PLoS ONE 9:E107637-E107637(2014).
CC -!- FUNCTION: Transcription activator (PubMed:24278028, PubMed:25268707).
CC Binds to 5'-CAACTGTC-3' and/or 5'-TAACAAA-3' motif in target gene
CC promoter (e.g. alpha-amylase) to promote their expression
CC (PubMed:11743113). Positive regulator of abscisic acid (ABA) responses
CC leading to growth arrest during seed germination (PubMed:17217461).
CC Promotes the expression of aleurone-related genes (e.g. CP1, CP, GASA1,
CC BXL1 and BXL2) in seeds. Together with MYB33 and MYB65, promotes the
CC programmed cell death (PCD) leading to vacuolation of protein storage
CC vacuoles (PSVs) in the aleurone layers during seed germination
CC (PubMed:20699403). Maybe involved in the regulation of leaves lamina
CC morphogenesis (PubMed:25268707). Involved in pollen grain development
CC (PubMed:22101548). Together with MYB97 and MYB120, functions as a male
CC factor that controls pollen tube-synergid interaction in fertilization.
CC Required for pollen tube growth arrest and sperm cell release in the
CC female gametophyte, probably via the regulation of pollen tube-specific
CC gene expression (PubMed:24278028, PubMed:23791732).
CC {ECO:0000269|PubMed:11743113, ECO:0000269|PubMed:17217461,
CC ECO:0000269|PubMed:20699403, ECO:0000269|PubMed:22101548,
CC ECO:0000269|PubMed:23791732, ECO:0000269|PubMed:24278028,
CC ECO:0000269|PubMed:25268707}.
CC -!- INTERACTION:
CC O80883; Q9FYF9: At1g67340; NbExp=3; IntAct=EBI-15215062, EBI-591197;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:23791732, ECO:0000269|PubMed:24278028,
CC ECO:0000269|PubMed:25268707}. Note=Detected in the vegetative nucleus
CC of mature pollen grains and pollen tubes.
CC {ECO:0000269|PubMed:23791732}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=O80883-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O80883-2; Sequence=VSP_058816;
CC Name=3;
CC IsoId=O80883-3; Sequence=VSP_058815;
CC -!- TISSUE SPECIFICITY: Present mostly in flowers, siliques and floral
CC shoot tips (PubMed:11743113, PubMed:25268707). Expression is restricted
CC to the subapical pith cells of both vegetative and flowering plants and
CC to the hypocotyl hook (PubMed:11743113). Expressed in pollen grains and
CC pollen tube (PubMed:23791732, PubMed:24278028). Mostly expressed in
CC mature pollen grains, and, to a lower extent, in inflorescences and
CC siliques (PubMed:24278028). {ECO:0000269|PubMed:11743113,
CC ECO:0000269|PubMed:23791732, ECO:0000269|PubMed:24278028,
CC ECO:0000269|PubMed:25268707}.
CC -!- DEVELOPMENTAL STAGE: Present in a small patch of cells on the innermost
CC side of the hypocotyl hook of the germinating seedling, in the
CC subapical pith cells of plants growing vegetatively, in a similar zone
CC of expanding cells both in developing inflorescence stems, and below
CC mature flowers and elongating siliques (PubMed:11743113). Accumulates
CC in the pollen tube nucleus during pollen tube growth through the pistil
CC (PubMed:23791732). {ECO:0000269|PubMed:11743113,
CC ECO:0000269|PubMed:23791732}.
CC -!- INDUCTION: Repressed in germinating seeds by microRNA159 (miR159)-
CC mediated cleavage in an abscisic acid (ABA) and ABI3-dependent manner,
CC probably to desensitize hormone signaling during seedling stress
CC responses (PubMed:15226253, PubMed:17217461). Induced by increased upon
CC gibberellic acid (GA) treatment (PubMed:20699403).
CC {ECO:0000269|PubMed:15226253, ECO:0000269|PubMed:17217461,
CC ECO:0000269|PubMed:20699403}.
CC -!- DISRUPTION PHENOTYPE: Hyposensitivity to abscisic acid (ABA)
CC (PubMed:17217461). Reduced expression levels of aleurone-related genes
CC (e.g. CP1, CP, GASA1, BXL1 and BXL2) in seeds. The triple mutant myb33
CC myb65 myb101 has a male sterility and exhibits slower protein storage
CC vacuoles (PSVs) vacuolation rate in aleurone layers upon seed
CC germination (PubMed:20699403). Reduced production of abnormal pollen
CC grains with misarranged male germ unit (MGU) (PubMed:22101548). The
CC triple mutant myb97 myb101 myb120 is impaired in pollen tube growth
CC arrest and subsequent sperm cell release in the female gametophyte thus
CC leading to a drastically reduced fertility. Altered pollen tube-
CC specific gene expression (PubMed:23791732, PubMed:24278028).
CC {ECO:0000269|PubMed:17217461, ECO:0000269|PubMed:20699403,
CC ECO:0000269|PubMed:22101548, ECO:0000269|PubMed:23791732,
CC ECO:0000269|PubMed:24278028}.
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DR EMBL; AF411970; AAL58845.1; -; mRNA.
DR EMBL; AY519576; AAS10046.1; -; mRNA.
DR EMBL; AC004681; AAC25928.1; -; Genomic_DNA.
DR EMBL; AC005700; AAM15072.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08688.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08689.1; -; Genomic_DNA.
DR EMBL; AK319141; BAH57256.1; -; mRNA.
DR EMBL; AK229342; BAF01205.1; -; mRNA.
DR EMBL; BT026381; ABH04488.1; -; mRNA.
DR PIR; T02545; T02545.
DR RefSeq; NP_001077993.1; NM_001084524.1. [O80883-2]
DR RefSeq; NP_180805.1; NM_128805.4. [O80883-1]
DR AlphaFoldDB; O80883; -.
DR SMR; O80883; -.
DR IntAct; O80883; 4.
DR STRING; 3702.AT2G32460.1; -.
DR PaxDb; O80883; -.
DR PRIDE; O80883; -.
DR EnsemblPlants; AT2G32460.1; AT2G32460.1; AT2G32460. [O80883-1]
DR EnsemblPlants; AT2G32460.2; AT2G32460.2; AT2G32460. [O80883-2]
DR GeneID; 817807; -.
DR Gramene; AT2G32460.1; AT2G32460.1; AT2G32460. [O80883-1]
DR Gramene; AT2G32460.2; AT2G32460.2; AT2G32460. [O80883-2]
DR KEGG; ath:AT2G32460; -.
DR Araport; AT2G32460; -.
DR TAIR; locus:2060241; AT2G32460.
DR eggNOG; KOG0048; Eukaryota.
DR InParanoid; O80883; -.
DR OMA; HQGIGND; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; O80883; -.
DR PRO; PR:O80883; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80883; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; TAS:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IMP:UniProtKB.
DR GO; GO:0009789; P:positive regulation of abscisic acid-activated signaling pathway; IMP:TAIR.
DR GO; GO:0043068; P:positive regulation of programmed cell death; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:1990019; P:protein storage vacuole organization; IMP:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:1901371; P:regulation of leaf morphogenesis; IMP:UniProtKB.
DR GO; GO:0080092; P:regulation of pollen tube growth; IMP:UniProtKB.
DR GO; GO:0009739; P:response to gibberellin; IEP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation.
FT CHAIN 1..490
FT /note="Transcription factor MYB101"
FT /id="PRO_0000439245"
FT DOMAIN 15..67
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 68..122
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 43..67
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 95..118
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 168..207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 168..194
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..97
FT /note="Missing (in isoform 3)"
FT /id="VSP_058815"
FT VAR_SEQ 1..14
FT /note="MDGGGETTATATME -> MV (in isoform 2)"
FT /id="VSP_058816"
FT CONFLICT 314
FT /note="S -> N (in Ref. 1; AAL58845)"
FT /evidence="ECO:0000305"
FT CONFLICT 383
FT /note="N -> H (in Ref. 1; AAL58845)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 490 AA; 54437 MW; D91A8A86FA3D5FC9 CRC64;
MDGGGETTAT ATMEGRGLKK GPWTTTEDAI LTEYVRKHGE GNWNAVQKNS GLLRCGKSCR
LRWANHLRPN LKKGSFTPDE EKIIIDLHAK LGNKWARMAS QLPGRTDNEI KNYWNTRMKR
RQRAGLPLYP HEIQHQGIDI DDEFEFDLTS FQFQNQDLDH NHQNMIQYTN SSNTSSSSSS
FSSSSSQPSK RLRPDPLVST NPGLNPIPDS SMDFQMFSLY NNSLENDNNQ FGFSVPLSSS
SSSNEVCNPN HILEYISENS DTRNTNKKDI DAMSYSSLLM GDLEIRSSSF PLGLDNSVLE
LPSNQRPTHS FSSSPIIDNG VHLEPPSGNS GLLDALLEES QALSRGGLFK DVRVSSSDLC
EVQDKRVKMD FENLLIDHLN SSNHSSLGAN PNIHNKYNEP TMVKVTVDDD DELLTSLLNN
FPSTTTPLPD WYRVTEMQNE ASYLAPPSGI LMGNHQGNGR VEPPTVPPSS SVDPMASLGS
CYWSNMPSIC
