ID   MB12A_BOVIN             Reviewed;         273 AA.
AC   Q3T0N1;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Multivesicular body subunit 12A;
DE   AltName: Full=ESCRT-I complex subunit MVB12A;
DE   AltName: Full=Protein FAM125A;
GN   Name=MVB12A; Synonyms=FAM125A;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular
CC       trafficking process. Required for the sorting of endocytic
CC       ubiquitinated cargos into multivesicular bodies. May be involved in the
CC       ligand-mediated internalization and down-regulation of EGF receptor (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex
CC       required for transport I) which consists of TSG101, VPS28, a VPS37
CC       protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry.
CC       Interacts with CD2AP and CIN85/SH3KBP1. Interacts with CD2AP (via one
CC       of the SH3 domains). Interacts with TSG101; the association appears to
CC       be mediated by the TSG101-VPS37 binary subcomplex. Interacts with
CC       VPS28. Interacts with VPS37B; the association appears to be mediated by
CC       the TSG101-VPS37 binary subcomplex. Interacts with VPS37C; the
CC       association appears to be mediated by the TSG101-VPS37 binary
CC       subcomplex. Interacts with VPS37D; the association appears to be
CC       mediated by the TSG101-VPS37 binary subcomplex. Interacts with CEP55
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Nucleus
CC       {ECO:0000250}. Endosome. Cytoplasm, cytoskeleton, microtubule
CC       organizing center, centrosome. Late endosome membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}. Note=Colocalizes with F-
CC       actin. Some fraction may be nuclear (By similarity). {ECO:0000250}.
CC   -!- PTM: Phosphorylated on Tyr-204 upon EGF stimulation. Phosphorylation is
CC       required for interaction with CD2AP and CIN85/SH3KBP1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MVB12 family. {ECO:0000305}.
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DR   EMBL; BC102323; AAI02324.1; -; mRNA.
DR   RefSeq; NP_001029455.1; NM_001034283.2.
DR   AlphaFoldDB; Q3T0N1; -.
DR   SMR; Q3T0N1; -.
DR   STRING; 9913.ENSBTAP00000005111; -.
DR   iPTMnet; Q3T0N1; -.
DR   PaxDb; Q3T0N1; -.
DR   PRIDE; Q3T0N1; -.
DR   Ensembl; ENSBTAT00000005111; ENSBTAP00000005111; ENSBTAG00000003914.
DR   GeneID; 507199; -.
DR   KEGG; bta:507199; -.
DR   CTD; 93343; -.
DR   VEuPathDB; HostDB:ENSBTAG00000003914; -.
DR   VGNC; VGNC:31766; MVB12A.
DR   eggNOG; KOG4000; Eukaryota.
DR   GeneTree; ENSGT00940000160542; -.
DR   HOGENOM; CLU_064823_2_0_1; -.
DR   InParanoid; Q3T0N1; -.
DR   OMA; EYNYTFA; -.
DR   OrthoDB; 1021387at2759; -.
DR   TreeFam; TF314477; -.
DR   Proteomes; UP000009136; Chromosome 7.
DR   Bgee; ENSBTAG00000003914; Expressed in laryngeal cartilage and 105 other tissues.
DR   GO; GO:0005813; C:centrosome; IEA:Ensembl.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0000813; C:ESCRT I complex; IBA:GO_Central.
DR   GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0008289; F:lipid binding; IEA:Ensembl.
DR   GO; GO:0017124; F:SH3 domain binding; IEA:UniProtKB-KW.
DR   GO; GO:0043130; F:ubiquitin binding; IEA:Ensembl.
DR   GO; GO:0032510; P:endosome to lysosome transport via multivesicular body sorting pathway; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0032801; P:receptor catabolic process; IBA:GO_Central.
DR   GO; GO:0042058; P:regulation of epidermal growth factor receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0046755; P:viral budding; IBA:GO_Central.
DR   GO; GO:0019075; P:virus maturation; IBA:GO_Central.
DR   InterPro; IPR023341; MABP.
DR   InterPro; IPR040335; MVB12A.
DR   InterPro; IPR018798; MVB12A/B.
DR   InterPro; IPR023340; UMA.
DR   PANTHER; PTHR31612; PTHR31612; 1.
DR   Pfam; PF10240; DUF2464; 1.
DR   PROSITE; PS51498; MABP; 1.
DR   PROSITE; PS51497; UMA; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoskeleton; Endosome; Membrane; Nucleus; Phosphoprotein;
KW   Protein transport; Reference proteome; SH3-binding; Transport.
FT   CHAIN           1..273
FT                   /note="Multivesicular body subunit 12A"
FT                   /id="PRO_0000249068"
FT   DOMAIN          9..151
FT                   /note="MABP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00831"
FT   DOMAIN          215..265
FT                   /note="UMA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00830"
FT   REGION          154..186
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          192..273
FT                   /note="Interaction with TSG101, VPS37B and VPS28"
FT                   /evidence="ECO:0000250"
FT   MOTIF           155..160
FT                   /note="SH3-binding"
FT   MOD_RES         130
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT   MOD_RES         163
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT   MOD_RES         170
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT   MOD_RES         195
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT   MOD_RES         202
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT   MOD_RES         204
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT   MOD_RES         207
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96EY5"
SQ   SEQUENCE   273 AA;  28906 MW;  5E91A8CD03BA36E9 CRC64;
     MDPGPGANGM PLAGLAWSSA SAPPPRGFSA ISCTVEGTPA SFGKTFAQKS GYFLCLNPLG
     SLENPQENVV VDIQILVDKS PLPPGFSPVC DPLDSKASVS KKKRMCVKLV PLGAADTAVF
     DIRLSGKTKT VPGYLRVGDM GGFAIWCRKA KAPRPVPKPR ALSRDVRDLS LDSPGQPSKG
     GFPERTLSRL GSRASTLRRN DSIYEASNLY GISAMDGVPF TLHPRFEGKS CGPLAFSAFA
     DLTIKSLADI EAEYNYGFVV EKTAAARLPP SVS