MB12A_MOUSE
ID MB12A_MOUSE Reviewed; 271 AA.
AC Q78HU3;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Multivesicular body subunit 12A;
DE AltName: Full=ESCRT-I complex subunit MVB12A;
DE AltName: Full=Protein FAM125A;
GN Name=Mvb12a; Synonyms=Fam125a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II, and FVB/N; TISSUE=Liver, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200 AND TYR-202, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular
CC trafficking process. Required for the sorting of endocytic
CC ubiquitinated cargos into multivesicular bodies. May be involved in the
CC ligand-mediated internalization and down-regulation of EGF receptor (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex
CC required for transport I) which consists of TSG101, VPS28, a VPS37
CC protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry.
CC Interacts with CD2AP and CIN85/SH3KBP1. Interacts with CD2AP (via one
CC of the SH3 domains). Interacts with TSG101; the association appears to
CC be mediated by the TSG101-VPS37 binary subcomplex. Interacts with
CC VPS28. Interacts with VPS37B; the association appears to be mediated by
CC the TSG101-VPS37 binary subcomplex. Interacts with VPS37C; the
CC association appears to be mediated by the TSG101-VPS37 binary
CC subcomplex. Interacts with VPS37D; the association appears to be
CC mediated by the TSG101-VPS37 binary subcomplex. Interacts with CEP55
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Nucleus
CC {ECO:0000250}. Endosome. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome. Late endosome membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}. Note=Colocalizes with F-
CC actin. Some fraction may be nuclear (By similarity). {ECO:0000250}.
CC -!- PTM: Phosphorylated on Tyr-202 upon EGF stimulation. Phosphorylation is
CC required for interaction with CD2AP and CIN85/SH3KBP1 (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MVB12 family. {ECO:0000305}.
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DR EMBL; BC020119; AAH20119.1; -; mRNA.
DR EMBL; BC027419; AAH27419.1; -; mRNA.
DR EMBL; BC087734; AAH87734.1; -; mRNA.
DR CCDS; CCDS22398.1; -.
DR RefSeq; NP_082893.1; NM_028617.2.
DR AlphaFoldDB; Q78HU3; -.
DR SMR; Q78HU3; -.
DR BioGRID; 216204; 1.
DR STRING; 10090.ENSMUSP00000034272; -.
DR iPTMnet; Q78HU3; -.
DR PhosphoSitePlus; Q78HU3; -.
DR EPD; Q78HU3; -.
DR jPOST; Q78HU3; -.
DR MaxQB; Q78HU3; -.
DR PaxDb; Q78HU3; -.
DR PeptideAtlas; Q78HU3; -.
DR PRIDE; Q78HU3; -.
DR ProteomicsDB; 252738; -.
DR Antibodypedia; 51512; 35 antibodies from 11 providers.
DR Ensembl; ENSMUST00000034272; ENSMUSP00000034272; ENSMUSG00000031813.
DR GeneID; 73711; -.
DR KEGG; mmu:73711; -.
DR UCSC; uc009mdt.1; mouse.
DR CTD; 93343; -.
DR MGI; MGI:1920961; Mvb12a.
DR VEuPathDB; HostDB:ENSMUSG00000031813; -.
DR eggNOG; KOG4000; Eukaryota.
DR GeneTree; ENSGT00940000160542; -.
DR HOGENOM; CLU_064823_2_0_1; -.
DR InParanoid; Q78HU3; -.
DR OMA; EYNYTFA; -.
DR OrthoDB; 1021387at2759; -.
DR PhylomeDB; Q78HU3; -.
DR TreeFam; TF314477; -.
DR Reactome; R-MMU-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR BioGRID-ORCS; 73711; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Mvb12a; mouse.
DR PRO; PR:Q78HU3; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q78HU3; protein.
DR Bgee; ENSMUSG00000031813; Expressed in right kidney and 243 other tissues.
DR ExpressionAtlas; Q78HU3; baseline and differential.
DR Genevisible; Q78HU3; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0000813; C:ESCRT I complex; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0031982; C:vesicle; ISO:MGI.
DR GO; GO:0008289; F:lipid binding; ISO:MGI.
DR GO; GO:0017124; F:SH3 domain binding; IEA:UniProtKB-KW.
DR GO; GO:0043130; F:ubiquitin binding; ISO:MGI.
DR GO; GO:0032510; P:endosome to lysosome transport via multivesicular body sorting pathway; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0032801; P:receptor catabolic process; IBA:GO_Central.
DR GO; GO:0042058; P:regulation of epidermal growth factor receptor signaling pathway; ISO:MGI.
DR GO; GO:0046755; P:viral budding; ISO:MGI.
DR GO; GO:0019075; P:virus maturation; ISO:MGI.
DR InterPro; IPR023341; MABP.
DR InterPro; IPR040335; MVB12A.
DR InterPro; IPR018798; MVB12A/B.
DR InterPro; IPR023340; UMA.
DR PANTHER; PTHR31612; PTHR31612; 1.
DR Pfam; PF10240; DUF2464; 1.
DR PROSITE; PS51498; MABP; 1.
DR PROSITE; PS51497; UMA; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Endosome; Membrane; Nucleus; Phosphoprotein;
KW Protein transport; Reference proteome; SH3-binding; Transport.
FT CHAIN 1..271
FT /note="Multivesicular body subunit 12A"
FT /id="PRO_0000249070"
FT DOMAIN 7..149
FT /note="MABP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00831"
FT DOMAIN 213..263
FT /note="UMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00830"
FT REGION 149..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..271
FT /note="Interaction with TSG101, VPS37B and VPS28"
FT /evidence="ECO:0000250"
FT MOTIF 153..158
FT /note="SH3-binding"
FT MOD_RES 128
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT MOD_RES 161
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT MOD_RES 168
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT MOD_RES 193
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96EY5"
FT MOD_RES 200
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 202
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 205
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96EY5"
SQ SEQUENCE 271 AA; 28705 MW; 92749C457B4BB95D CRC64;
MDPGTDSAPL AGLVWSSASA PPPPGFSAIT CTVEGGTASF GRGFAQKAGY FLCLSTLGIP
ENPQDNVVVD MQIVMDKGPL PSGFSAVNDP QDIKASVSKK KRMCVKLMPL GTADVVVSDV
KLSGKTKTVP GYLRVGDIGG FAIWCKKSKA PRPVPKPRTL SQDMRGLSLD PPKEPSKGSH
PERTLSRLGS RASTLRRTDS IYEASSLYGI SAMDGVPFTL HPRFEGKSCG PLNLSAFGDL
TIKSLADIEK EYNYGFVVEK TAAARLPPSV S
