ID   MB12A_XENLA             Reviewed;         275 AA.
AC   Q7ZYJ7;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Multivesicular body subunit 12A;
DE   AltName: Full=ESCRT-I complex subunit MVB12A;
DE   AltName: Full=Protein FAM125A;
GN   Name=mvb12a; Synonyms=fam125a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo, and Oocyte;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular
CC       trafficking process. Required for the sorting of endocytic
CC       ubiquitinated cargos into multivesicular bodies (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex
CC       required for transport I). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC       Late endosome membrane {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}. Note=Colocalizes with F-actin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MVB12 family. {ECO:0000305}.
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DR   EMBL; BC043757; AAH43757.1; -; mRNA.
DR   EMBL; BC110944; AAI10945.1; -; mRNA.
DR   RefSeq; NP_001079473.1; NM_001086004.1.
DR   AlphaFoldDB; Q7ZYJ7; -.
DR   SMR; Q7ZYJ7; -.
DR   BioGRID; 97403; 1.
DR   IntAct; Q7ZYJ7; 1.
DR   MaxQB; Q7ZYJ7; -.
DR   DNASU; 379160; -.
DR   GeneID; 379160; -.
DR   KEGG; xla:379160; -.
DR   CTD; 379160; -.
DR   Xenbase; XB-GENE-6251626; mvb12a.L.
DR   OrthoDB; 1021387at2759; -.
DR   Proteomes; UP000186698; Chromosome 8L.
DR   Bgee; 379160; Expressed in zone of skin and 19 other tissues.
DR   GO; GO:0000813; C:ESCRT I complex; IEA:InterPro.
DR   GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0017124; F:SH3 domain binding; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR023341; MABP.
DR   InterPro; IPR040335; MVB12A.
DR   InterPro; IPR018798; MVB12A/B.
DR   InterPro; IPR023340; UMA.
DR   PANTHER; PTHR31612; PTHR31612; 1.
DR   Pfam; PF10240; DUF2464; 1.
DR   PROSITE; PS51498; MABP; 1.
DR   PROSITE; PS51497; UMA; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Endosome; Membrane; Protein transport; Reference proteome;
KW   SH3-binding; Transport.
FT   CHAIN           1..275
FT                   /note="Multivesicular body subunit 12A"
FT                   /id="PRO_0000249073"
FT   DOMAIN          5..145
FT                   /note="MABP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00831"
FT   DOMAIN          216..267
FT                   /note="UMA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00830"
FT   MOTIF           151..156
FT                   /note="SH3-binding"
SQ   SEQUENCE   275 AA;  29897 MW;  68399FD902DE4D74 CRC64;
     MENSSTPITG LAWISSVTLC PKTYNMIVAT VEGASANFVK GFNQKSAVYL GYSTVPEGLE
     QVITDIQMLN EKTVLPVGYA FIGEHLDPKI SVPKKKRLCV KQMPMHTAET AVCEIKLSMK
     SKQFGAPYER IGEISGLVFW CRKGSVSAPK PTPKPRNITS GIKGLSLDSN TAVQPVRRAS
     APNPDAGQAV PLSNGKWGLS ENAIYDSSNI YGISAIDGIP FTIHPMFENT INNSSVAASD
     FRDLHIKTLS EIESEYNYGF VVEKTAAARI PPRLR