MB12B_MOUSE
ID MB12B_MOUSE Reviewed; 317 AA.
AC Q6KAU4; A2ARF1; Q6PB42;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Multivesicular body subunit 12B;
DE AltName: Full=ESCRT-I complex subunit MVB12B;
DE AltName: Full=Protein FAM125B;
GN Name=Mvb12b; Synonyms=Fam125b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Fetal brain;
RX PubMed=15449545; DOI=10.1093/dnares/11.2.127;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of FLJ genes: the
RT complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified
RT by screening of terminal sequences of cDNA clones randomly sampled from
RT size-fractionated libraries.";
RL DNA Res. 11:127-135(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-222, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular
CC trafficking process. Required for the sorting of endocytic
CC ubiquitinated cargos into multivesicular bodies (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex
CC required for transport I) which consists of TSG101, VPS28, a VPS37
CC protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry.
CC Interacts with TSG101; the association appears to be mediated by the
CC TSG101-VPS37 binary subcomplex. Interacts with VPS28. Interacts with
CC VPS37B; the association appears to be mediated by the TSG101-VPS37
CC binary subcomplex. Interacts with VPS37C; the association appears to be
CC mediated by the TSG101-VPS37 binary subcomplex (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250}. Late endosome membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MVB12 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD21363.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK131113; BAD21363.1; ALT_INIT; mRNA.
DR EMBL; AL845360; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX649361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC049129; AAH49129.2; -; mRNA.
DR EMBL; BC059907; AAH59907.1; -; mRNA.
DR CCDS; CCDS15946.1; -.
DR RefSeq; NP_780393.2; NM_175184.4.
DR AlphaFoldDB; Q6KAU4; -.
DR SMR; Q6KAU4; -.
DR BioGRID; 215427; 3.
DR STRING; 10090.ENSMUSP00000048901; -.
DR iPTMnet; Q6KAU4; -.
DR PhosphoSitePlus; Q6KAU4; -.
DR SwissPalm; Q6KAU4; -.
DR jPOST; Q6KAU4; -.
DR MaxQB; Q6KAU4; -.
DR PaxDb; Q6KAU4; -.
DR PeptideAtlas; Q6KAU4; -.
DR PRIDE; Q6KAU4; -.
DR ProteomicsDB; 292273; -.
DR Antibodypedia; 56615; 23 antibodies from 11 providers.
DR DNASU; 72543; -.
DR Ensembl; ENSMUST00000041555; ENSMUSP00000048901; ENSMUSG00000038740.
DR GeneID; 72543; -.
DR KEGG; mmu:72543; -.
DR UCSC; uc008jib.1; mouse.
DR CTD; 89853; -.
DR MGI; MGI:1919793; Mvb12b.
DR VEuPathDB; HostDB:ENSMUSG00000038740; -.
DR eggNOG; KOG4000; Eukaryota.
DR GeneTree; ENSGT00940000155945; -.
DR HOGENOM; CLU_064823_1_0_1; -.
DR InParanoid; Q6KAU4; -.
DR OMA; ICFSKTE; -.
DR OrthoDB; 1021387at2759; -.
DR PhylomeDB; Q6KAU4; -.
DR TreeFam; TF314477; -.
DR Reactome; R-MMU-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR BioGRID-ORCS; 72543; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Mvb12b; mouse.
DR PRO; PR:Q6KAU4; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q6KAU4; protein.
DR Bgee; ENSMUSG00000038740; Expressed in rostral migratory stream and 243 other tissues.
DR ExpressionAtlas; Q6KAU4; baseline and differential.
DR Genevisible; Q6KAU4; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005769; C:early endosome; ISO:MGI.
DR GO; GO:0000813; C:ESCRT I complex; ISO:MGI.
DR GO; GO:0005770; C:late endosome; ISO:MGI.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0031982; C:vesicle; ISO:MGI.
DR GO; GO:0008289; F:lipid binding; ISO:MGI.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042058; P:regulation of epidermal growth factor receptor signaling pathway; ISO:MGI.
DR GO; GO:0046755; P:viral budding; ISO:MGI.
DR GO; GO:0019075; P:virus maturation; ISO:MGI.
DR InterPro; IPR023341; MABP.
DR InterPro; IPR018798; MVB12A/B.
DR InterPro; IPR040297; MVB12B.
DR InterPro; IPR023340; UMA.
DR PANTHER; PTHR31547; PTHR31547; 1.
DR Pfam; PF10240; DUF2464; 1.
DR PROSITE; PS51498; MABP; 1.
DR PROSITE; PS51497; UMA; 1.
PE 1: Evidence at protein level;
KW Endosome; Membrane; Phosphoprotein; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..317
FT /note="Multivesicular body subunit 12B"
FT /id="PRO_0000249075"
FT DOMAIN 45..191
FT /note="MABP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00831"
FT DOMAIN 252..301
FT /note="UMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00830"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 193..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..25
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 195..218
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..317
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 99
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P6"
FT MOD_RES 120
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P6"
FT MOD_RES 202
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P6"
FT MOD_RES 203
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P6"
FT MOD_RES 222
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 307
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P6"
SQ SEQUENCE 317 AA; 35411 MW; 0FF01346C82C7998 CRC64;
MRSCFCVRRS RDPPPPQPPP PQRGTDQATM PEVKELSEAL PETPMDPITG VGVVASRNRA
PTGYDVVAQT ADGVDADLWK DGLFKSKVTR YLCFTRSFSK ENSHLGNVLV DMKLIDVKDT
LPVGFIPIQE TVDTQEVVFR KKRLCIKFIP RDSTEAAICD IRIMGRTKQA PPQYTFIGEL
NSMGIWYRMG RVPRNHDSSQ PTTPSQSSAS STPAPNLPRH ISLTLPATFR GRNNTSTDYE
YQLSNLYAIS AMDGVPFMIS EKFSCIPESM QPFDLLGITI KSLAEIEKEY EYSFRTEQSA
AARLPPSPTR CQQIPQS
