MB3R2_ORYSJ
ID MB3R2_ORYSJ Reviewed; 587 AA.
AC Q0JHU7; D6BV30; Q5N9U1; Q5QT31;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Transcription factor MYB3R-2 {ECO:0000305};
DE AltName: Full=Myb-related protein MYB3R-2 {ECO:0000305};
DE Short=OsMYB3R-2 {ECO:0000303|PubMed:17293435};
GN Name=MYB3R-2 {ECO:0000303|PubMed:17293435};
GN Synonyms=MYB15 {ECO:0000312|EMBL:CAD22534.1};
GN OrderedLocusNames=Os01g0841500 {ECO:0000312|EMBL:BAF06681.1},
GN LOC_Os01g62410 {ECO:0000305};
GN ORFNames=OsJ_04034 {ECO:0000312|EMBL:EEE55651.1},
GN P0408C03.41 {ECO:0000312|EMBL:BAD81765.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Xu S., Huang W., Xu Z., Luo D., Xue H.;
RT "Isolation of Osmyb15, a rice MYB protein with novel structure, playing
RT possible significant roles during evolution from plant MYB3R to R2R3 MYB.";
RL Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Ilpoombyeo; TISSUE=Seed;
RA Yoon U.H., Lee G.S., Lee J.S., Hahn J.H., Kim C.K., Yun D.W., Kim Y.H.;
RT "Oryza sativa japonica group clone KCS635B12 Myb-like protein mRNA,
RT complete cds.";
RL Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=17293435; DOI=10.1104/pp.106.094532;
RA Dai X., Xu Y., Ma Q., Xu W., Wang T., Xue Y., Chong K.;
RT "Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to
RT freezing, drought, and salt stress in transgenic Arabidopsis.";
RL Plant Physiol. 143:1739-1751(2007).
RN [9]
RP FUNCTION.
RX PubMed=19279197; DOI=10.1104/pp.108.133454;
RA Ma Q., Dai X., Xu Y., Guo J., Liu Y., Chen N., Xiao J., Zhang D., Xu Z.,
RA Zhang X., Chong K.;
RT "Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is
RT mediated by alteration in cell cycle and ectopic expression of stress
RT genes.";
RL Plant Physiol. 150:244-256(2009).
CC -!- FUNCTION: Transcription factor involved in abiotic stress responses
CC (PubMed:17293435, PubMed:19279197). May play a regulatory role in
CC tolerance to salt, cold, and drought stresses (PubMed:17293435).
CC Transcriptional activator that binds specifically to a mitosis-specific
CC activator cis-element 5'-(T/C)C(T/C)AACGG(T/C)(T/C)A-3', found in
CC promoters of cyclin genes such as CYCB1-1 and KNOLLE (AC Q84R43).
CC Positively regulates a subset of G2/M phase-specific genes, including
CC CYCB1-1, CYCB2-1, CYCB2-2, and CDC20.1 in response to cold treatment
CC (PubMed:19279197). {ECO:0000269|PubMed:17293435,
CC ECO:0000269|PubMed:19279197}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:17293435}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0JHU7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0JHU7-2; Sequence=VSP_058917;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems and spikes.
CC {ECO:0000269|PubMed:17293435}.
CC -!- INDUCTION: Induced by cold, drought and salt stresses.
CC {ECO:0000269|PubMed:17293435}.
CC -!- MISCELLANEOUS: Plants over-expressing MYB3R-2 show increased tolerance
CC to freezing (PubMed:19279197). Plants over-expressing MYB3R-2 show
CC retarded growth, increased tolerance to cold, drought and salt
CC stresses, and have decreased sensitivity to seed germination inhibition
CC by abscisic acid (ABA) or salt (PubMed:17293435).
CC {ECO:0000269|PubMed:17293435, ECO:0000269|PubMed:19279197}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD81765.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=EEE55651.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ430052; CAD22534.1; -; mRNA.
DR EMBL; FJ940212; ADB84634.1; -; mRNA.
DR EMBL; FJ940219; ADB84641.1; -; mRNA.
DR EMBL; AP003241; BAD81765.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP008207; BAF06681.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS75160.1; -; Genomic_DNA.
DR EMBL; CM000138; EEE55651.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_015616493.1; XM_015761007.1. [Q0JHU7-1]
DR RefSeq; XP_015616500.1; XM_015761014.1. [Q0JHU7-1]
DR AlphaFoldDB; Q0JHU7; -.
DR SMR; Q0JHU7; -.
DR STRING; 4530.OS01T0841500-01; -.
DR PaxDb; Q0JHU7; -.
DR PRIDE; Q0JHU7; -.
DR EnsemblPlants; Os01t0841500-01; Os01t0841500-01; Os01g0841500. [Q0JHU7-1]
DR GeneID; 4327399; -.
DR Gramene; Os01t0841500-01; Os01t0841500-01; Os01g0841500. [Q0JHU7-1]
DR KEGG; osa:4327399; -.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_016150_3_1_1; -.
DR InParanoid; Q0JHU7; -.
DR OMA; IAECFPY; -.
DR OrthoDB; 1499244at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:1901002; P:positive regulation of response to salt stress; IMP:UniProtKB.
DR GO; GO:1902584; P:positive regulation of response to water deprivation; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009409; P:response to cold; IMP:UniProtKB.
DR CDD; cd00167; SANT; 3.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR Pfam; PF00249; Myb_DNA-binding; 3.
DR SMART; SM00717; SANT; 3.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS51294; HTH_MYB; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome; Repeat;
KW Stress response; Transcription; Transcription regulation.
FT CHAIN 1..587
FT /note="Transcription factor MYB3R-2"
FT /id="PRO_0000439824"
FT DOMAIN 62..113
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 114..169
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 170..220
FT /note="HTH myb-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 90..113
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 142..165
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 193..216
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 14..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 441..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..58
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..93
FT /note="MGAMAMVEQEGCVENRQPLAASSSSVSDGSSYGGGGGGLAQMSPPVSSSANS
FT ISGLRRTSGPIRRAKGGWTPEEDETLRKAVEAYKGRNWKKI -> MYLYA (in
FT isoform 2)"
FT /id="VSP_058917"
FT CONFLICT 199
FT /note="V -> A (in Ref. 2; ADB84641 and 1; CAD22534)"
FT /evidence="ECO:0000305"
FT CONFLICT 460
FT /note="E -> G (in Ref. 2; ADB84641 and 1; CAD22534)"
FT /evidence="ECO:0000305"
FT CONFLICT 472
FT /note="Y -> H (in Ref. 2; ADB84641 and 1; CAD22534)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 587 AA; 64100 MW; 77C569D47EB8A3F2 CRC64;
MGAMAMVEQE GCVENRQPLA ASSSSVSDGS SYGGGGGGLA QMSPPVSSSA NSISGLRRTS
GPIRRAKGGW TPEEDETLRK AVEAYKGRNW KKIAECFPYR TEVQCLHRWQ KVLNPELIKG
PWTQEEDDQI IDLVKKYGPT KWSVIAKALP GRIGKQCRER WHNHLNPEIR KDAWTTEEEQ
ALINAHRIYG NKWAEIAKVL PGRTDNSIKN HWNSSLRKKQ DMYNTSNNMV VPKLLVHDKF
KDKPKLMAME GHLDLNKAPI INSKDQPGTA HRSNCSGFLS RSSLPTAQPL TSREASVVDG
SAVTLVAQAL ESDSVRGKGL EIDSVHEKGL EVNSAPDHTG NSWTIQLEAA PSKGEAELSL
KNEARSLGPL CYQIPNMEDV VPVSSSLFSD HLTGNHTSEH CGDDILSPAG CTTPPPTKGK
LTSQLSVDSI LKSAANSFPG TPSILKRRKR DKSTPVSASE MKISGSNTDR FYTPMGMEPA
TATPESFKTT SFLSLGSLDG SVKSFDVSPQ YRARSKRMAL TKTVEKQLDF SSDGLDTCGS
EILNSSCNNS QSTLSITEAP KLKEKEHAVQ LENLTKNFAH TTNLDVT
