MB3R4_ARATH
ID MB3R4_ARATH Reviewed; 961 AA.
AC Q94FL9; Q56WW6; Q9LYE0;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Transcription factor MYB3R-4 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 3R-4 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Protein ENHANCED DEFECTIVE CYTOKINESIS {ECO:0000303|PubMed:25806785};
GN Name=MYB3R4 {ECO:0000303|PubMed:11597504};
GN Synonyms=EDC {ECO:0000303|PubMed:25806785};
GN OrderedLocusNames=At5g11510 {ECO:0000312|Araport:AT5G11510};
GN ORFNames=F15N18.100 {ECO:0000312|EMBL:CAB87711.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: A genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 497-961.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=15937229; DOI=10.1105/tpc.105.031724;
RA Nawy T., Lee J.Y., Colinas J., Wang J.Y., Thongrod S.C., Malamy J.E.,
RA Birnbaum K., Benfey P.N.;
RT "Transcriptional profile of the Arabidopsis root quiescent center.";
RL Plant Cell 17:1908-1925(2005).
RN [7]
RP INDUCTION BY SALICYLIC ACID, AND GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=17287251; DOI=10.1242/dev.02801;
RA Haga N., Kato K., Murase M., Araki S., Kubo M., Demura T., Suzuki K.,
RA Mueller I., Voss U., Juergens G., Ito M.;
RT "R1R2R3-Myb proteins positively regulate cytokinesis through activation of
RT KNOLLE transcription in Arabidopsis thaliana.";
RL Development 134:1101-1110(2007).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY POWDERY MILDEW.
RC STRAIN=cv. Columbia;
RX PubMed=20018666; DOI=10.1073/pnas.0912492107;
RA Chandran D., Inada N., Hather G., Kleindt C.K., Wildermuth M.C.;
RT "Laser microdissection of Arabidopsis cells at the powdery mildew infection
RT site reveals site-specific processes and regulators.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:460-465(2010).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=21862669; DOI=10.1104/pp.111.180836;
RA Haga N., Kobayashi K., Suzuki T., Maeo K., Kubo M., Ohtani M., Mitsuda N.,
RA Demura T., Nakamura K., Juergens G., Ito M.;
RT "Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and
RT preferential down-regulation of multiple G2/M-specific genes in
RT Arabidopsis.";
RL Plant Physiol. 157:706-717(2011).
RN [11]
RP INTERACTION WITH CDKA-1; RBR1 AND E2FB, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=26069325; DOI=10.15252/embj.201490899;
RA Kobayashi K., Suzuki T., Iwata E., Nakamichi N., Suzuki T., Chen P.,
RA Ohtani M., Ishida T., Hosoya H., Mueller S., Leviczky T.,
RA Pettko-Szandtner A., Darula Z., Iwamoto A., Nomoto M., Tada Y.,
RA Higashiyama T., Demura T., Doonan J.H., Hauser M.T., Sugimoto K., Umeda M.,
RA Magyar Z., Boegre L., Ito M.;
RT "Transcriptional repression by MYB3R proteins regulates plant organ
RT growth.";
RL EMBO J. 34:1992-2007(2015).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=25806785; DOI=10.4161/15592324.2014.990817;
RA Saito T., Fujikawa H., Haga N., Suzuki T., Machida Y., Ito M.;
RT "Genetic interaction between G2/M phase-specific transcription factor
RT MYB3R4 and MAPKKK ANP3 for execution of cytokinesis in Arabidopsis
RT thaliana.";
RL Plant Signal. Behav. 10:E990817-E990817(2015).
CC -!- FUNCTION: Transcription factor that binds 5'-AACGG-3' motifs in gene
CC promoters (PubMed:21862669). Involved in the regulation of cytokinesis,
CC probably via the activation of several G2/M phase-specific genes
CC transcription (e.g. KNOLLE) (PubMed:17287251, PubMed:21862669,
CC PubMed:25806785). Required for the maintenance of diploidy
CC (PubMed:21862669). {ECO:0000269|PubMed:17287251,
CC ECO:0000269|PubMed:21862669, ECO:0000269|PubMed:25806785}.
CC -!- FUNCTION: Involved in transcription regulation during induced
CC endoreduplication at the powdery mildew (e.g. G.orontii) infection
CC site, thus promoting G.orontii growth and reproduction.
CC {ECO:0000269|PubMed:20018666}.
CC -!- SUBUNIT: Component of a DREAM-like complex which modulates a variety of
CC developmentally regulated genes and of the mitotic genes in
CC proliferating and differentiated cells. Associates with CDKA-1, RBR1
CC and E2FB, but not with E2FC, in proliferating cells, at early stages of
CC leaves development. {ECO:0000269|PubMed:26069325}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=Additional isoforms seem to exist. {ECO:0000305};
CC Name=1;
CC IsoId=Q94FL9-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in roots, cotyledons and leaves,
CC especially in vascular tissues, and in flowers.
CC {ECO:0000269|PubMed:17287251}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the root quiescent center
CC (PubMed:15937229). Accumulates in division zone of primary root tips
CC and emerging lateral roots. Also present in floral organs in young
CC flower buds. Weakly and uniformly present in the developing embryo and
CC maternal tissues (PubMed:17287251). Expressed specifically in
CC proliferating stage of leaves (PubMed:26069325).
CC {ECO:0000269|PubMed:15937229, ECO:0000269|PubMed:17287251,
CC ECO:0000269|PubMed:26069325}.
CC -!- INDUCTION: Slightly induced by salicylic acid (SA) (PubMed:16463103).
CC Expressed in a cell cycle-dependent manner, with highest levels 2 hours
CC before the peak of mitotic index in cells synchronized by aphidicolin.
CC Activated by CYCB1 (PubMed:17287251). Accumulates at powdery mildew
CC (e.g. G.orontii) infected cells. {ECO:0000269|PubMed:16463103,
CC ECO:0000269|PubMed:17287251}.
CC -!- DISRUPTION PHENOTYPE: In EDC plants, defects of cytokinesis
CC (PubMed:25806785). The double mutant myb3r1 myb3r4 often fails to
CC complete cytokinesis, resulting in multinucleate cells with gapped
CC walls and cell wall stubs in diverse tissues (e.g. in embryo during the
CC first or second division after fertilization, in stomata guard mother
CC cell) and several pleiotropic developmental defects, and associated
CC with the selective reduction of several G2/M phase-specific genes
CC transcript levels (e.g. CYCB2, CDC20.1 and KNOLLE). Hypersensitivity to
CC caffeine, an inhibitor of cytokinesis (PubMed:17287251,
CC PubMed:21862669). Impaired powdery mildew (e.g. G.orontii)-induced
CC endoreduplication. Reduced G.orontii growth and reproduction leading to
CC an enhanced resistance to powdery mildew (PubMed:20018666).
CC {ECO:0000269|PubMed:17287251, ECO:0000269|PubMed:20018666,
CC ECO:0000269|PubMed:21862669, ECO:0000269|PubMed:25806785}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB87711.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF371975; AAK54739.2; -; mRNA.
DR EMBL; AY519650; AAS10120.1; -; mRNA.
DR EMBL; AL163815; CAB87711.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91689.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68601.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68602.1; -; Genomic_DNA.
DR EMBL; AK221914; BAD94312.1; -; mRNA.
DR PIR; T48510; T48510.
DR RefSeq; NP_001330337.1; NM_001343197.1. [Q94FL9-1]
DR RefSeq; NP_001330338.1; NM_001343198.1. [Q94FL9-1]
DR RefSeq; NP_568249.1; NM_121189.4. [Q94FL9-1]
DR AlphaFoldDB; Q94FL9; -.
DR SMR; Q94FL9; -.
DR STRING; 3702.AT5G11510.1; -.
DR iPTMnet; Q94FL9; -.
DR PaxDb; Q94FL9; -.
DR PRIDE; Q94FL9; -.
DR ProteomicsDB; 238822; -. [Q94FL9-1]
DR EnsemblPlants; AT5G11510.1; AT5G11510.1; AT5G11510. [Q94FL9-1]
DR EnsemblPlants; AT5G11510.3; AT5G11510.3; AT5G11510. [Q94FL9-1]
DR EnsemblPlants; AT5G11510.4; AT5G11510.4; AT5G11510. [Q94FL9-1]
DR GeneID; 831023; -.
DR Gramene; AT5G11510.1; AT5G11510.1; AT5G11510. [Q94FL9-1]
DR Gramene; AT5G11510.3; AT5G11510.3; AT5G11510. [Q94FL9-1]
DR Gramene; AT5G11510.4; AT5G11510.4; AT5G11510. [Q94FL9-1]
DR KEGG; ath:AT5G11510; -.
DR Araport; AT5G11510; -.
DR TAIR; locus:2144216; AT5G11510.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_016150_0_1_1; -.
DR InParanoid; Q94FL9; -.
DR OMA; NNGVENR; -.
DR OrthoDB; 137792at2759; -.
DR PhylomeDB; Q94FL9; -.
DR PRO; PR:Q94FL9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q94FL9; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009524; C:phragmoplast; IDA:TAIR.
DR GO; GO:0005819; C:spindle; IDA:TAIR.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:UniProtKB.
DR GO; GO:1901181; P:negative regulation of cellular response to caffeine; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0032465; P:regulation of cytokinesis; IMP:UniProtKB.
DR GO; GO:0032875; P:regulation of DNA endoreduplication; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0009620; P:response to fungus; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR CDD; cd00167; SANT; 3.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR SMART; SM00717; SANT; 3.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS51294; HTH_MYB; 3.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation.
FT CHAIN 1..961
FT /note="Transcription factor MYB3R-4"
FT /id="PRO_0000438894"
FT DOMAIN 24..75
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 76..131
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 132..182
FT /note="HTH myb-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 52..75
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 104..127
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 155..178
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 390..457
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 534..555
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 756..781
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 612..619
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 417..443
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 534..549
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 961 AA; 108236 MW; E48FA4C3F7AB33BF CRC64;
MEAESSTPQE RIPKLRHGRT SGPARRSTRG QWTAEEDEIL RKAVHSFKGK NWKKIAEYFK
DRTDVQCLHR WQKVLNPELV KGPWTKEEDE MIVQLIEKYG PKKWSTIARF LPGRIGKQCR
ERWHNHLNPA INKEAWTQEE ELLLIRAHQI YGNRWAELTK FLPGRSDNGI KNHWHSSVKK
KLDSYMSSGL LDQYQAMPLA PYERSSTLQS TFMQSNIDGN GCLNGQAENE IDSRQNSSMV
GCSLSARDFQ NGTINIGHDF HPCGNSQENE QTAYHSEQFY YPELEDISVS ISEVSYDMED
CSQFPDHNVS TSPSQDYQFD FQELSDISLE MRHNMSEIPM PYTKESKEST LGAPNSTLNI
DVATYTNSAN VLTPETECCR VLFPDQESEG HSVSRSLTQE PNEFNQVDRR DPILYSSASD
RQISEATKSP TQSSSSRFTA TAASGKGTLR PAPLIISPDK YSKKSSGLIC HPFEVEPKCT
TNGNGSFICI GDPSSSTCVD EGTNNSSEED QSYHVNDPKK LVPVNDFASL AEDRPHSLPK
HEPNMTNEQH HEDMGASSSL GFPSFDLPVF NCDLLQSKND PLHDYSPLGI RKLLMSTMTC
MSPLRLWESP TGKKTLVGAQ SILRKRTRDL LTPLSEKRSD KKLEIDIAAS LAKDFSRLDV
MFDETENRQS NFGNSTGVIH GDRENHFHIL NGDGEEWSGK PSSLFSHRMP EETMHIRKSL
EKVDQICMEA NVREKDDSEQ DVENVEFFSG ILSEHNTGKP VLSTPGQSVT KAEKAQVSTP
RNQLQRTLMA TSNKEHHSPS SVCLVINSPS RARNKEGHLV DNGTSNENFS IFCGTPFRRG
LESPSAWKSP FYINSLLPSP RFDTDLTIED MGYIFSPGER SYESIGVMTQ INEHTSAFAA
FADAMEVSIS PTNDDARQKK ELDKENNDPL LAERRVLDFN DCESPIKATE EVSSYLLKGC
R
