MB3R5_ARATH
ID MB3R5_ARATH Reviewed; 548 AA.
AC Q6R032; Q94FL8; Q9LZ93;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Transcription factor MYB3R-5 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 3R-5 {ECO:0000303|PubMed:11597504};
GN Name=MYB3R5 {ECO:0000303|PubMed:11597504};
GN OrderedLocusNames=At5g02320 {ECO:0000312|Araport:AT5G02320};
GN ORFNames=T1E22.80 {ECO:0000312|EMBL:CAB85537.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: A genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP INDUCTION BY ETHYLENE AND SALICYLIC ACID, AND GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [6]
RP GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=17287251; DOI=10.1242/dev.02801;
RA Haga N., Kato K., Murase M., Araki S., Kubo M., Demura T., Suzuki K.,
RA Mueller I., Voss U., Juergens G., Ito M.;
RT "R1R2R3-Myb proteins positively regulate cytokinesis through activation of
RT KNOLLE transcription in Arabidopsis thaliana.";
RL Development 134:1101-1110(2007).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=26069325; DOI=10.15252/embj.201490899;
RA Kobayashi K., Suzuki T., Iwata E., Nakamichi N., Suzuki T., Chen P.,
RA Ohtani M., Ishida T., Hosoya H., Mueller S., Leviczky T.,
RA Pettko-Szandtner A., Darula Z., Iwamoto A., Nomoto M., Tada Y.,
RA Higashiyama T., Demura T., Doonan J.H., Hauser M.T., Sugimoto K., Umeda M.,
RA Magyar Z., Boegre L., Ito M.;
RT "Transcriptional repression by MYB3R proteins regulates plant organ
RT growth.";
RL EMBO J. 34:1992-2007(2015).
CC -!- FUNCTION: Transcription factor that binds 5'-AACGG-3' motifs in gene
CC promoters (By similarity). Transcription repressor that regulates organ
CC growth. Binds to the promoters of G2/M-specific genes and to E2F target
CC genes to prevent their expression in post-mitotic cells and to restrict
CC the time window of their expression in proliferating cells
CC (PubMed:26069325). {ECO:0000250|UniProtKB:Q94FL9,
CC ECO:0000269|PubMed:26069325}.
CC -!- SUBUNIT: Component of a DREAM-like complex which modulates a variety of
CC developmentally regulated genes and of the mitotic genes in
CC proliferating and differentiated cells. {ECO:0000250|UniProtKB:Q8H1P9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- DEVELOPMENTAL STAGE: Expressed both in proliferating and maturing
CC stages of leaves. {ECO:0000269|PubMed:26069325}.
CC -!- INDUCTION: Slightly induced by ethylene and salicylic acid (SA).
CC {ECO:0000269|PubMed:16463103}.
CC -!- DISRUPTION PHENOTYPE: In double mutant myb3r3 myb3r5 and triple mutant
CC myb3r1 myb3r3 myb3r5, up-regulation of many G2/M-specific genes leading
CC to larger seeds, organs and embryos due to overproliferation and
CC ectopic cell divisions. {ECO:0000269|PubMed:26069325}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB85537.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY519649; AAS10119.1; -; mRNA.
DR EMBL; AF371976; AAK54740.2; -; mRNA.
DR EMBL; AL162874; CAB85537.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90458.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90459.1; -; Genomic_DNA.
DR PIR; T48253; T48253.
DR RefSeq; NP_001190206.1; NM_001203277.2.
DR RefSeq; NP_568099.1; NM_120310.2.
DR AlphaFoldDB; Q6R032; -.
DR SMR; Q6R032; -.
DR IntAct; Q6R032; 5.
DR STRING; 3702.AT5G02320.1; -.
DR PaxDb; Q6R032; -.
DR PRIDE; Q6R032; -.
DR EnsemblPlants; AT5G02320.1; AT5G02320.1; AT5G02320.
DR EnsemblPlants; AT5G02320.2; AT5G02320.2; AT5G02320.
DR GeneID; 831802; -.
DR Gramene; AT5G02320.1; AT5G02320.1; AT5G02320.
DR Gramene; AT5G02320.2; AT5G02320.2; AT5G02320.
DR KEGG; ath:AT5G02320; -.
DR Araport; AT5G02320; -.
DR TAIR; locus:2180157; AT5G02320.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_016150_3_1_1; -.
DR InParanoid; Q6R032; -.
DR OMA; STHSPEY; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q6R032; -.
DR PRO; PR:Q6R032; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6R032; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009723; P:response to ethylene; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR CDD; cd00167; SANT; 3.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 3.
DR SMART; SM00717; SANT; 3.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS51294; HTH_MYB; 3.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..548
FT /note="Transcription factor MYB3R-5"
FT /id="PRO_0000438895"
FT DOMAIN 70..121
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 122..177
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 178..228
FT /note="HTH myb-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 98..121
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 150..173
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 201..224
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 60..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 255..288
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 512..548
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 86..93
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 264..283
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 3
FT /note="S -> L (in Ref. 2; AAK54740)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 548 AA; 61732 MW; D7E8EF3C98F46D03 CRC64;
MSSSSNPPVC SPEKEERSEM KIEIQCMENK QPLAASCSSA SEGSGCFFLK SPEIATPATV
SSFPRRTSGP MRRAKGGWTP EEDETLRRAV EKYKGKRWKK IAEFFPERTE VQCLHRWQKV
LNPELVKGPW TQEEDDKIVE LVKKYGPAKW SVIAKSLPGR IGKQCRERWH NHLNPGIRKD
AWTVEEESAL MNSHRMYGNK WAEIAKVLPG RTDNAIKNHW NSSLKKKLEF YLATGNLPPP
ASKFIVLKDI ADGDRDSKQS SATKPFKDSD SLTQTSSGNT DSNEVGRDHF DSSSALLEEV
AASRRIGVNE YACSPVEYKP QLPNLEPISE EVRINSKAYF ERSIQRKVEN GFGTPKHGNL
YYKSPLDYYF PSEADLQHMY GYECGCSPGA ASPVSLMTTP CNKDSGLTAT RSPESFLREA
ARTFPNTPSI FRKRRKVVLA AKTDAVVVVN GVVKEVDRKE ESKDMRKSLL LETTDNCSDD
EELGLNGNAF NLSPPYRLRA KRTAVIKSRQ LEFTSEKEKQ PDNEIEFTSA KEKQPDNEIK
TSEEDKPV
