MBB1A_DROME
ID MBB1A_DROME Reviewed; 1133 AA.
AC Q9W5E4; Q5BIF7; Q8MRJ3;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Myb-binding protein 1A {ECO:0000303|PubMed:29065309, ECO:0000312|FlyBase:FBgn0001341};
GN Name=Mybbp1A {ECO:0000303|PubMed:29065309,
GN ECO:0000312|FlyBase:FBgn0001341};
GN Synonyms=l(1)Bi {ECO:0000312|FlyBase:FBgn0001341};
GN ORFNames=CG6189 {ECO:0000312|FlyBase:FBgn0001341};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAM50234.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM50234.1}; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:AAX33415.1, ECO:0000312|EMBL:AGV77189.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAX33415.1, ECO:0000312|EMBL:AGV77189.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAX33415.1};
RA Carlson J., Booth B., Chavez C., Frise E., George R., Pacleb J., Park S.,
RA Wan K., Yu C., Rubin G.M., Stapleton M., Celniker S.E.;
RL Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, INTERACTION WITH NCLB, AND DISRUPTION PHENOTYPE.
RX PubMed=29065309; DOI=10.1016/j.devcel.2017.09.022;
RA Liu Y., Mattila J., Ventelae S., Yadav L., Zhang W., Lamichane N.,
RA Sundstroem J., Kauko O., Grenman R., Varjosalo M., Westermarck J.,
RA Hietakangas V.;
RT "PWP1 Mediates Nutrient-Dependent Growth Control through Nucleolar
RT Regulation of Ribosomal Gene Expression.";
RL Dev. Cell 43:240-252(2017).
CC -!- FUNCTION: Has a role in rRNA biogenesis, cell proliferation and tissue
CC growth by contributing to the localization of nclb to the nucleolus.
CC {ECO:0000269|PubMed:29065309}.
CC -!- SUBUNIT: Interacts with nclb. {ECO:0000269|PubMed:29065309}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BQG0}. Nucleus
CC {ECO:0000305|PubMed:29065309}. Nucleus, nucleolus
CC {ECO:0000305|PubMed:29065309}.
CC -!- DISRUPTION PHENOTYPE: Reduced rRNA transcription which results in
CC larval reduced growth (PubMed:29065309). RNAi-mediated knockdown in the
CC fat body results in the mislocalization of nclb from the nucleolus to
CC the nucleoplasm (PubMed:29065309). {ECO:0000269|PubMed:29065309}.
CC -!- SIMILARITY: Belongs to the MYBBP1A family. {ECO:0000305}.
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DR EMBL; AE014298; AAF45532.1; -; Genomic_DNA.
DR EMBL; AY119580; AAM50234.1; -; mRNA.
DR EMBL; BT021267; AAX33415.1; -; mRNA.
DR EMBL; BT150288; AGV77189.1; -; mRNA.
DR PIR; T13384; T13384.
DR RefSeq; NP_476593.2; NM_057245.3.
DR AlphaFoldDB; Q9W5E4; -.
DR IntAct; Q9W5E4; 1.
DR STRING; 7227.FBpp0070114; -.
DR PaxDb; Q9W5E4; -.
DR PRIDE; Q9W5E4; -.
DR EnsemblMetazoa; FBtr0070119; FBpp0070114; FBgn0001341.
DR GeneID; 31010; -.
DR KEGG; dme:Dmel_CG6189; -.
DR UCSC; CG6189-RA; d. melanogaster.
DR CTD; 10514; -.
DR FlyBase; FBgn0001341; Mybbp1A.
DR VEuPathDB; VectorBase:FBgn0001341; -.
DR eggNOG; KOG1926; Eukaryota.
DR HOGENOM; CLU_275239_0_0_1; -.
DR InParanoid; Q9W5E4; -.
DR OMA; LQTGHFW; -.
DR OrthoDB; 86051at2759; -.
DR PhylomeDB; Q9W5E4; -.
DR BioGRID-ORCS; 31010; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 31010; -.
DR PRO; PR:Q9W5E4; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0001341; Expressed in eye disc (Drosophila) and 22 other tissues.
DR ExpressionAtlas; Q9W5E4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR GO; GO:0090070; P:positive regulation of ribosome biogenesis; IDA:FlyBase.
DR GO; GO:1901838; P:positive regulation of transcription of nucleolar large rRNA by RNA polymerase I; IDA:FlyBase.
DR GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR007015; DNA_pol_V/MYBBP1A.
DR PANTHER; PTHR13213; PTHR13213; 1.
DR Pfam; PF04931; DNA_pol_phi; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Ribosome biogenesis.
FT CHAIN 1..1133
FT /note="Myb-binding protein 1A"
FT /evidence="ECO:0000305"
FT /id="PRO_0000445266"
FT REGION 1..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 718..764
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 924..943
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1111..1133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..58
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 729..762
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 263
FT /note="D -> N (in Ref. 4; AAX33415)"
FT /evidence="ECO:0000305"
FT CONFLICT 817
FT /note="P -> S (in Ref. 4; AAX33415)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1133 AA; 127867 MW; 642AB8F07629073A CRC64;
MKSKVDKPVT NGAPNATKTK AKEDRKRAKT QKSEADSQIP AKILKKSKKE KPAETEEKEN
TASNANLKVS QINKAVFVVF KKMQGSQLTK KMINSLITLL RDDTNAEQRT ATTGYVLKRL
IRSTGADDMK TVSLAASYIH CILNAVPAID AFEVLETLKR DLAVGSQQRG KEDSLAAVGQ
LVTAFCILQT PQFAKAEPKL VTAVFQILAA QLKGREYLVS LCGDILAVSF KQLPAAIFEE
YVWPLLQPEL NKPLSGLKVN TCDVLLAVHL TFSSVLGREN ILASLWPKKP VYTQLFDLYF
SGSTIHSDGV YARLASFLVN GGKDMLAAWQ QYIDSKQPLK LNAAKACAIQ VLSHVLLNFK
PREEQLILDI FTPTCVQFLL HECSSVKWDK GEGKKPSLKK LKEICFKFEG SLVLCYEKQF
QNDDNKLHLL LKLLEHTLQL DSVISLPRFC QKLINQLSVE SLHKLYDYYN NKLYSLEDED
RVSRVHCLNQ MQLILNHSKL SQTAKWRQKQ LNYLLLAGLF HLDASKKPCE AAQASAFSRQ
CAARCEEIFL GSLLHKCSGL PGLCQLLQKT LSYLNKELGQ PDAESKLRSP RDESLQKAWK
QVEKLLERPS KESDVVGQSF EALILFVSLA LCTKIPPSIT VLEDLIICRK NALQKSKEQV
NEELKWQDVL TDALLQLLLQ TGHFWREFVQ LVATALIPHL EHGNLEQVLE VLNMNKNPLS
KKSDGEEESD DELDKEESLK DSSDDSEDED EDEEEDEGED DAESHLAQIR ESVRQALVND
GDADDDGASS VDWNDVDEEQ GERLNAALER SFQMFRPKSR KAQEKERPTK SERIDSTNLL
HFRIRALDLL ELFITKKPTQ SVILDVLHCV FQVYSHCAAD SKLQSLREAS LKLLKKILAR
NIELKENQST APILEAIEQL MSSGEEHSEE DQENGKQPAS RQAKRDIIIW RDRCFAYLVS
QASADGEPKK SAVWPLLVEF LELWVAKRRS RLSLASFEAL FQSGQWQGVA PLAVVLASHL
DVQKTRSFRR AQILKLLSEQ GRRLEVAFKD NNSSSKKFEK QIARYVNQLE TKASSSKELN
LLLKILAQGG EKWQKLREQI QLVAKDLQPN KKVAKQKKQA AAKPMVVEDE EST
