ID   MBCT_MYCTO              Reviewed;         186 AA.
AC   P9WLP8; L0TB64; P64907; Q10869;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=NAD(+) phosphorylase MbcT {ECO:0000250|UniProtKB:P9WLP9};
DE            EC=2.4.2.- {ECO:0000250|UniProtKB:P9WLP9};
DE   AltName: Full=Mycobacterial cidal toxin MbcT {ECO:0000250|UniProtKB:P9WLP9};
GN   Name=mbcT; OrderedLocusNames=MT2043;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [2]
RP   INDUCTION IN MOUSE INFECTION MODEL.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=20628579; DOI=10.1371/journal.ppat.1000988;
RA   Homolka S., Niemann S., Russell D.G., Rohde K.H.;
RT   "Functional genetic diversity among Mycobacterium tuberculosis complex
RT   clinical isolates: delineation of conserved core and lineage-specific
RT   transcriptomes during intracellular survival.";
RL   PLoS Pathog. 6:E1000988-E1000988(2010).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Degrades NAD(+) by phosphorolysis. Neutralized by its cognate antitoxin
CC       MbcA. {ECO:0000250|UniProtKB:P9WLP9}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + phosphate = ADP-beta-D-ribose 1''-phosphate + H(+) +
CC         nicotinamide; Xref=Rhea:RHEA:20788, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17154, ChEBI:CHEBI:43474, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:58753; Evidence={ECO:0000250|UniProtKB:P9WLP9};
CC   -!- SUBUNIT: Forms a heterotetramer with cognate antitoxin MbcA.
CC       {ECO:0000250|UniProtKB:P9WLP9}.
CC   -!- INDUCTION: Expression induced in both active and resting C57BL/6 mouse
CC       macrophages. {ECO:0000269|PubMed:20628579}.
CC   -!- SIMILARITY: Belongs to the MbcT/ParT/Res family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000516; AAK46319.1; -; Genomic_DNA.
DR   PIR; C70757; C70757.
DR   RefSeq; WP_003410001.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WLP8; -.
DR   SMR; P9WLP8; -.
DR   EnsemblBacteria; AAK46319; AAK46319; MT2043.
DR   KEGG; mtc:MT2043; -.
DR   PATRIC; fig|83331.31.peg.2200; -.
DR   HOGENOM; CLU_124933_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR014914; RES_dom.
DR   Pfam; PF08808; RES; 1.
DR   SMART; SM00953; RES; 1.
PE   2: Evidence at transcript level;
KW   NAD; Nucleotidyltransferase; Toxin-antitoxin system; Transferase.
FT   CHAIN           1..186
FT                   /note="NAD(+) phosphorylase MbcT"
FT                   /id="PRO_0000427441"
SQ   SEQUENCE   186 AA;  20281 MW;  8414F65A43BD909D CRC64;
     MSDALDEGLV QRIDARGTIE WSETCYRYTG AHRDALSGEG ARRFGGRWNP PLLFPAIYLA
     DSAQACMVEV ERAAQAASTT AEKMLEAAYR LHTIDVTDLA VLDLTTPQAR EAVGLENDDI
     YGDDWSGCQA VGHAAWFLHM QGVLVPAAGG VGLVVTAYEQ RTRPGQLQLR QSVDLTPALY
     QELRAT