MBD5_MOUSE
ID MBD5_MOUSE Reviewed; 1498 AA.
AC B1AYB6;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Methyl-CpG-binding domain protein 5;
DE AltName: Full=Methyl-CpG-binding protein MBD5;
GN Name=Mbd5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=20700456; DOI=10.1371/journal.pone.0011982;
RA Laget S., Joulie M., Le Masson F., Sasai N., Christians E., Pradhan S.,
RA Roberts R.J., Defossez P.A.;
RT "The human proteins MBD5 and MBD6 associate with heterochromatin but they
RT do not bind methylated DNA.";
RL PLoS ONE 5:E11982-E11982(2010).
CC -!- FUNCTION: Binds to heterochromatin. Does not interact with either
CC methylated or unmethylated DNA (in vitro) (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome {ECO:0000250}.
CC Note=Associated with pericentric heterochromatin. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:20700456}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryo at 7, 11, 15 and 17 dpc.
CC {ECO:0000269|PubMed:20700456}.
CC -!- DOMAIN: Both MBD and PWWP domains are necessary for chromocentric
CC localization. {ECO:0000250}.
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DR EMBL; AL845298; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX546465; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC138375; AAI38376.1; -; mRNA.
DR EMBL; BC138378; AAI38379.1; -; mRNA.
DR CCDS; CCDS71051.1; -.
DR RefSeq; NP_001277585.1; NM_001290656.1.
DR RefSeq; XP_017170404.1; XM_017314915.1.
DR AlphaFoldDB; B1AYB6; -.
DR SMR; B1AYB6; -.
DR BioGRID; 224612; 1.
DR IntAct; B1AYB6; 1.
DR iPTMnet; B1AYB6; -.
DR PhosphoSitePlus; B1AYB6; -.
DR PaxDb; B1AYB6; -.
DR PRIDE; B1AYB6; -.
DR ProteomicsDB; 287319; -.
DR Antibodypedia; 33627; 115 antibodies from 19 providers.
DR Ensembl; ENSMUST00000112754; ENSMUSP00000108374; ENSMUSG00000036792.
DR GeneID; 109241; -.
DR KEGG; mmu:109241; -.
DR UCSC; uc012bve.1; mouse.
DR CTD; 55777; -.
DR MGI; MGI:2138934; Mbd5.
DR VEuPathDB; HostDB:ENSMUSG00000036792; -.
DR eggNOG; ENOG502QTC7; Eukaryota.
DR GeneTree; ENSGT00530000064137; -.
DR HOGENOM; CLU_003105_0_0_1; -.
DR InParanoid; B1AYB6; -.
DR Reactome; R-MMU-5689603; UCH proteinases.
DR BioGRID-ORCS; 109241; 2 hits in 45 CRISPR screens.
DR ChiTaRS; Mbd5; mouse.
DR PRO; PR:B1AYB6; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; B1AYB6; protein.
DR Bgee; ENSMUSG00000036792; Expressed in animal zygote and 220 other tissues.
DR ExpressionAtlas; B1AYB6; baseline and differential.
DR Genevisible; B1AYB6; MM.
DR GO; GO:0010369; C:chromocenter; ISO:MGI.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0030496; C:midbody; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0042593; P:glucose homeostasis; IMP:MGI.
DR GO; GO:0007399; P:nervous system development; ISO:MGI.
DR GO; GO:0060399; P:positive regulation of growth hormone receptor signaling pathway; IMP:MGI.
DR GO; GO:0050795; P:regulation of behavior; ISO:MGI.
DR GO; GO:0040014; P:regulation of multicellular organism growth; IMP:MGI.
DR InterPro; IPR037385; MBD5/MBD6.
DR InterPro; IPR001739; Methyl_CpG_DNA-bd.
DR PANTHER; PTHR16112; PTHR16112; 1.
DR SMART; SM00391; MBD; 1.
DR PROSITE; PS50982; MBD; 1.
PE 2: Evidence at transcript level;
KW Chromosome; Nucleus; Reference proteome.
FT CHAIN 1..1498
FT /note="Methyl-CpG-binding domain protein 5"
FT /id="PRO_0000406940"
FT DOMAIN 11..81
FT /note="MBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00338"
FT DOMAIN 1389..1451
FT /note="PWWP"
FT REGION 199..274
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 329..350
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 452..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 594..642
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1350..1377
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1473..1498
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..236
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 256..271
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..346
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 455..471
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 497..512
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 594..634
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1362..1377
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1498 AA; 160292 MW; A7B2A2D483A84DCE CRC64;
MNGGKECDGG DKEGGLAAIQ VPVGWQRRVD HNGVLYISPS GSLLSCLDQV KTYLLTDGTC
KCGLECPLIL PKVFNFDPGA AVKQRTAEDV KADDDVTKLC IHKRKIIAVA TLHQSMEAPH
PSLVLTSPGG GTNATPVVPS RAATPRSVRN KSHEGITNSV MPECKNPFKL MTGSSNAMGR
LYMQDLPGSQ QQELHPVYPR QRLGSSEHGQ KSPFRGSHGG LPSPASSGSQ IYGDGSISPR
TDPLGSPDVF TRNNPGFHGA PNSSPIHLNR TPLSPPSVML HGSPVQSSCA MAGRTNIPLS
PTLTTKSPVM KKPMCNFSTN MEIPRAMFHH KPPQGPPPPP PPSCALQKKP LTSEKDPLGI
LDPIPSKPVN QNPIIINPTS FHSNVHSQVP VMNVSMPPAV VPLPSNLPLP TVKPGHMNHG
SHVQRIQHSA STSLSPSPVT SPVHMMGTGI GRIEASPQRS RSSSTSSDHG NFMMPPVGPQ
ATCSGIKVPP RSPRSTIGSP RPSMPSSPST KSDGHHQYKD IPNPLIAGMS NVLNTPSSAA
FPTAPAGNGS VKSQPGLLGM PLNQILNQHN AASFPASSLL SAAAKAQLAN QNKLAGNNSS
SSNNSGAVAS SGNTEGHSTL NTMFPPTANM LLPTGEGQSG RAALRDKLMS QQKDSLRKRK
QPPTTVLSLL RQSQMDSSAA PKPGPDLLRK HGQGSFPISS MSQLLQSMSC QSSHLSSNST
PGCGGSNTAL PCSANQLHFP DPNMNSTVLQ NSLTQSIPLR GEAVHCHNAN TNFVHSNSPV
PNHHLAGLIN QIQASGNCGM LSQSGMALGN SLHPNPPQSR ISTSSTPVIP NSIVSSYNQT
SSEAGGSSLP SSIAIAGSNH PAITKTTSVL QDGVIVTTAA GNPLQSQLPI GSDFPFVGQE
HALHFPSNST ANNHLPHPLN PSLLSSLPIS LPVNQQHLLN QNLLNILQPS AGEGDISSIN
NSLNNHQLTH LQSLLNSNQM FPPNQQPQQH LLQGHQNLQA FQGQPTVPCP ANNNPMACLF
QNFQVRMQGD AALLNKRIST QPGLTTLPEN PNLALPHFQD TPCELQPRID LGQPMKDGLV
MGGQGDASVD AIYKAVVDAA SKGMQVVITT AVNSTTQISP IPALSAMSAF TASIGDPLNL
SSAVSAVIHG RNMGGVDHDG RLRNARGARL PKNIDHGKNS SEGDGFECFK SASCHTSRKQ
WDGEQSPRGE RNRWKYEEFL DHPGHIHSSP CHERPNNVST LPFLAGEQHP ILLPPRNCQG
DKILEENFRY NNYKRTMMSF KERLESTVER CTHINGNRPR QSRGFGELLG TAKQDLVLEG
QSPGSSNSLE SSLVKDYIHY NGDFNAKTIN GCVPSPSDAK SISSEDDLRN PDSPSSHELI
HYRPRTFNVG DLVWGQIKGL TSWPGKFIRE DDVHNSCQQS PEEGKVEPEK LKTLTEGLEA
YSRVRKRSRK SGKLNNHLEA AIHEAMSELD KMSGTVHQIP QGDRQMRPPK PKRRKISR
