MBD6_MOUSE
ID MBD6_MOUSE Reviewed; 1003 AA.
AC Q3TY92; E9PWH8;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Methyl-CpG-binding domain protein 6;
DE AltName: Full=Methyl-CpG-binding protein MBD6;
GN Name=Mbd6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Visual cortex;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=20700456; DOI=10.1371/journal.pone.0011982;
RA Laget S., Joulie M., Le Masson F., Sasai N., Christians E., Pradhan S.,
RA Roberts R.J., Defossez P.A.;
RT "The human proteins MBD5 and MBD6 associate with heterochromatin but they
RT do not bind methylated DNA.";
RL PLoS ONE 5:E11982-E11982(2010).
CC -!- FUNCTION: Binds to heterochromatin. Does not interact with either
CC methylated or unmethylated DNA (in vitro) (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome {ECO:0000250}.
CC Note=Associated with pericentric heterochromatin in about 25% of the
CC cells. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Expressed at highest levels in adult
CC testis and brain. {ECO:0000269|PubMed:20700456}.
CC -!- DEVELOPMENTAL STAGE: Detected in embryo from 7 to 17 dpc.
CC {ECO:0000269|PubMed:20700456}.
CC -!- DOMAIN: The MBD domain is necessary for chromocentric localization.
CC {ECO:0000250}.
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DR EMBL; AK158797; BAE34671.1; -; mRNA.
DR EMBL; AC144852; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS83762.1; -.
DR RefSeq; NP_001334100.1; NM_001347171.1.
DR RefSeq; NP_149063.2; NM_033072.3.
DR AlphaFoldDB; Q3TY92; -.
DR SMR; Q3TY92; -.
DR STRING; 10090.ENSMUSP00000026476; -.
DR iPTMnet; Q3TY92; -.
DR PhosphoSitePlus; Q3TY92; -.
DR PaxDb; Q3TY92; -.
DR PRIDE; Q3TY92; -.
DR ProteomicsDB; 252740; -.
DR Antibodypedia; 50110; 53 antibodies from 14 providers.
DR DNASU; 110962; -.
DR Ensembl; ENSMUST00000119078; ENSMUSP00000112805; ENSMUSG00000025409.
DR GeneID; 110962; -.
DR KEGG; mmu:110962; -.
DR UCSC; uc007hix.1; mouse.
DR CTD; 114785; -.
DR MGI; MGI:106378; Mbd6.
DR VEuPathDB; HostDB:ENSMUSG00000025409; -.
DR eggNOG; ENOG502QR6I; Eukaryota.
DR GeneTree; ENSGT00530000064137; -.
DR InParanoid; Q3TY92; -.
DR OrthoDB; 75997at2759; -.
DR Reactome; R-MMU-5689603; UCH proteinases.
DR BioGRID-ORCS; 110962; 8 hits in 76 CRISPR screens.
DR ChiTaRS; Mbd6; mouse.
DR PRO; PR:Q3TY92; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q3TY92; protein.
DR Bgee; ENSMUSG00000025409; Expressed in floor plate of midbrain and 247 other tissues.
DR ExpressionAtlas; Q3TY92; baseline and differential.
DR Genevisible; Q3TY92; MM.
DR GO; GO:0010369; C:chromocenter; ISO:MGI.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0001650; C:fibrillar center; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR InterPro; IPR037385; MBD5/MBD6.
DR InterPro; IPR001739; Methyl_CpG_DNA-bd.
DR PANTHER; PTHR16112; PTHR16112; 1.
DR SMART; SM00391; MBD; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS50982; MBD; 1.
PE 2: Evidence at transcript level;
KW Chromosome; Nucleus; Reference proteome.
FT CHAIN 1..1003
FT /note="Methyl-CpG-binding domain protein 6"
FT /id="PRO_0000406941"
FT DOMAIN 13..83
FT /note="MBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00338"
FT REGION 123..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 241..302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 334..619
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 631..659
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 684..736
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 753..1003
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..197
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..294
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 389..409
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 445..460
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 572..593
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 689..703
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 704..736
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 807..844
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 872..890
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 905..922
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 946..960
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 961..975
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 122
FT /note="T -> S (in Ref. 1; BAE34671)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1003 AA; 100990 MW; 7F0605DD73D836E8 CRC64;
MNGDNASSAA DRAGGPAATP VPIPIGWQRC VREGAVYYIS PSGTELSSLE QTRSYLLSDG
TCKCGLECPL NVPKVFNFDP LAPVTPGGAG VGPASEEDMT KLCNHRRKAV AMATLYRSME
TTCSHSSPGE GASPQMFHTV SPGPPSVRPP CRAPPTTPLN GGPGSIPQDP PSVPQAFPPL
TGPAGLFPPP RLPDPVPSAG SSSPCFLPRG NAPSPAPPPP PAISLNAPSY NWGASLRSNL
VPSDLGSPPA PHASSSPPSD SPLFHCSDAL TSPPLPPSNN PPGPPGPPGP ATQPPVSSAT
MHLPLVLGSL GGAPAVEGPG APPFLASSLL SAAAKAQLPP PSTLQGRRPR AQAPSAAHAS
PRPSQRRPRR PPTVLRLLEG GGPQTPRRTR PRAPAPVPQP FPLPEPSQPI LPSVLSLLGL
PTPGPSHSDG SFNLLGSDAH LPPPPALSSG SPPQPRHPIQ PSLPGTTSGS LSSVPGAPAP
PAASKAPVVP SPVLQSPSDG LGMGAGPACP LPPLAGGEAF PFPSPEQGLA LSGAGFPGML
GALPLPLSLG QPPPSPFLSH SLFGVLAGGG QPPPEPLLPP PGGPGPPSAP GEPEGPSLLV
ASLLSPPPSD LLPPPSAPPS NLLASFLPLL ALGPTAGDGE GSAEGAGGPN GEPFSGLGDL
PPLLFPPLSA PPTLIALNSA LLAASLDPPS GTPPQPCVLS APQPGPPTSS VTTATTDPGA
SSLGKAPSNS GRPQLLSPLL SASLLGDLSS LASSPGALPS LLQPPGPLLS SQLGLQLLPG
GGAPPALSEA SSPLACLLQS LQIPPEQPDA PCLPPESPAS ALEPEPARPP LSALAPPHAS
PDPPVPELLT GRGSGKRGRR GGGGLRGING ETRPGRGRKP GSRREPGRLA LKWGTRGGFN
GQMERSPRRT HHWQHNGELA EGGAEPKDPP LPGTHSEDLK VPPGIVRKSR RGRRRKYNPA
RNSSSSRQDV TLEPSPTTRA AVPLPPRARP GRPAKNKRRK LAP
